We narrowed to 469 results for: LARGE
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TypeBlog Post[email protected]. These deposits are considered large deposits and are exceptions to our standard workflow...
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Plasmids 101: 5 factors to help you choose the right cloning method
TypeBlog Post...could be good choices if you have a particularly large number of inserts to assemble. You could also consider... -
3D Printing Meets CRISPR Cas9
TypeBlog Post...Steitz was presented with a physical model of his large ribosomal subunit, he kept sliding his palm across...the models we create are around 30 million times larger than the real thing. Nevertheless, the models are... -
Fluorescent Proteins 101: Introduction to FRET
TypeBlog Post...the possibilities can seem overwhelming given the large assortment of fluorescent proteins available. Fortunately... -
Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells
TypeBlog Post...sequence ranging from a single nucleotide change to large insertions like the addition of a fluorophore or... -
Hot Plasmids and Viral Preps - January 2021
TypeBlog Post...cells with unique spatiotemporal phenotypes from large heterogeneous cultures. HeLa cells expressed...without any reduction in performance compared to larger genome-wide libraries. Find the library here ... -
Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015
TypeBlog Post...CRISPR variety (see slides from my talk below). Largely organized by Dmitry Samarsky, and a very welcoming... -
Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)
TypeBlog Post...in my lab notebook from time to time. I wrote in large letters “Going for it!” We kept our day jobs and... -
Technical Design of a Western Blot
TypeBlog Post...Conversely, if your protein of interest is large, look for a larger pore size (0.45 µm). Types of transfers...to overnight to run, and is recommended for very large proteins. A semi-dry transfer is faster — 3–30 minutes... -
Pooled Library Amplifications
TypeBlog Post... prone to recombination, while a library with a large backbone might be more difficult to transform. ...generally needs to be determined experimentally. For larger libraries that require hundreds of nanograms of...full-size plasmids (or for smaller plasmids to overtake larger plasmids, if the library varies significantly in... -
Viral Vectors 101: Voltage Indicators
TypeBlog Post... This specific example is attractive due to the large number of sheer molecules and signal you can generate... -
Viral Vectors 101: Chemogenetics
TypeBlog Post.... Picking receptors The receptor you choose is largely dependent on what cellular response you aim to ... -
Deep Dive: Fixing and Permeabilizing for Immunofluorescence
TypeBlog Post...Permeabilization What is the point? Antibodies are large proteins that need a little help crossing cell membranes... -
Summer SciComm Series: Modes of Communication
TypeBlog Post...microscopy) Visually compelling. Easy to convey a large number of details without overwhelming your audience... -
PCR Applications for Copy Number Variation Assays
TypeBlog Post...amplicons. Sybr has high background noise, due to the large amount of dsDNA in most starting materials, and ... -
A Guide to Starting Your Own Journal Club
TypeBlog Post...interest from relevant parties, and the company at large, by polling and informal communications we made ...winding down. Perhaps other projects have taken on a larger priority for the participants. Perhaps people feel... -
xCas9: Engineering a CRISPR Variant with PAM Flexibility
TypeBlog Post...system, the mutagenesis plasmid (MP6) permits quick, large-scale mutation of the gene of interest. They created... -
Truncated gRNAs for Regulating Gene Expression
TypeBlog Post...gRNAs Importantly, the use of truncated guides has largely been demonstrated to result in decreased mismatch... -
CRISPR 101: Ribonucleoprotein (RNP) Delivery
TypeBlog Post... homology directed repair (HDR), and generating large genomic deletions. What are the advantages of Cas9... -
Adenoviral Vector Production and Troubleshooting
TypeBlog Post...can be produced at high titers, can accommodate large transgenes, transduce quiescent and dividing cells...