We narrowed to 464 results for: LARGE
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TypeBlog Post...indicating that Met protein functions have been largely conserved since the species diverged. Tips on using...
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Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools
TypeBlog Post... commonly observed with direct stimulation are largely absent in the soCoChR-expressing system. Taken ... -
Fluorescent Proteins 101: Introduction to FRET
TypeBlog Post...the possibilities can seem overwhelming given the large assortment of fluorescent proteins available. Fortunately... -
Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015
TypeBlog Post...CRISPR variety (see slides from my talk below). Largely organized by Dmitry Samarsky, and a very welcoming... -
Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)
TypeBlog Post...in my lab notebook from time to time. I wrote in large letters “Going for it!” We kept our day jobs and... -
Technical Design of a Western Blot
TypeBlog Post...Conversely, if your protein of interest is large, look for a larger pore size (0.45 µm). Types of transfers...to overnight to run, and is recommended for very large proteins. A semi-dry transfer is faster — 3–30 minutes... -
Pooled Library Amplifications
TypeBlog Post... prone to recombination, while a library with a large backbone might be more difficult to transform. ...generally needs to be determined experimentally. For larger libraries that require hundreds of nanograms of...full-size plasmids (or for smaller plasmids to overtake larger plasmids, if the library varies significantly in... -
Plasmids 101: Degron Tags
TypeBlog Post...proteasome for degradation. The HaloTag is quite large (33 kDa) and does not self-cleave off of the protein... -
Viral Vectors 101: Voltage Indicators
TypeBlog Post... This specific example is attractive due to the large number of sheer molecules and signal you can generate... -
Viral Vectors 101: Chemogenetics
TypeBlog Post.... Picking receptors The receptor you choose is largely dependent on what cellular response you aim to ... -
Uncovering Ancestral Bias in CRISPR Research
TypeBlog Post...groups or, by extension, race. Many researchers do large-scale CRISPR screens in cancer cell lines, looking...address this issue, but it still points to a far larger and more complex problem endemic to biomedical ... -
Truncated gRNAs for Regulating Gene Expression
TypeBlog Post...gRNAs Importantly, the use of truncated guides has largely been demonstrated to result in decreased mismatch... -
A Guide to Starting Your Own Journal Club
TypeBlog Post...interest from relevant parties, and the company at large, by polling and informal communications we made ...winding down. Perhaps other projects have taken on a larger priority for the participants. Perhaps people feel... -
xCas9: Engineering a CRISPR Variant with PAM Flexibility
TypeBlog Post...system, the mutagenesis plasmid (MP6) permits quick, large-scale mutation of the gene of interest. They created... -
The Scientific Conference Poster Session: Tips for Success
TypeBlog Post...you are interested in viewing which has drawn a large crowd, making it difficult to see the actual poster...posters you are interested in viewing. For most larger conferences, poster titles and abstracts are available... -
CRISPR 101: Ribonucleoprotein (RNP) Delivery
TypeBlog Post... homology directed repair (HDR), and generating large genomic deletions. What are the advantages of Cas9... -
Adenoviral Vector Production and Troubleshooting
TypeBlog Post...can be produced at high titers, can accommodate large transgenes, transduce quiescent and dividing cells... -
CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!
TypeBlog Post...relies on antibody chains, which are relatively large and are not expressed consistently throughout cells... -
AAV Vector Quality Control: Going the Extra Mile with NGS
TypeBlog Post...believe this isn’t cause for concern. However, a large number of hits to one sequence/gene - we set our... -
Cloning Mammalian Cells with the Agarose Method
TypeBlog Post... advantage of this technique is the potentially large number of single clones obtained (on occasion we...