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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 497 results for: RAP

Showing: 261 - 280 of 497 results

  2. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ... genes with potentially harmful mutations in therapeutic settings. Silencing of a target gene is achieved...end_module_attribute %}{% module_attribute "wrap_field_tag" is_json="true" %}{% raw %}"div"{% endraw %...

  3. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ... (SpyTag-GFP nanobody) assembled on beads. C) Micrograph of H1.8-GFP nucleosomes collected with MagIC-...of glutaraldehyde crosslinking: representative micrograph (C), particle orientation distribution and Fourier-shell...

  4. Evolution of Lab Techniques

    Type
    Blog Post
    ...labeling it. The label can then be visualized by autoradiography and X-ray film for radioactively labeled probes...commonplace in laboratories. As scientific technologies rapidly progress, some common lab techniques we use now...

  5. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    ...universities are trying to compile more comprehensive demographic information about the postdoc work force, problems...sorted out there can be gaps in knowledge of demographics and the information is often confidential (see...

  6. General Transfection

    Type
    Protocol
    ...-Tip The pH of this solution will drift pretty rapidly upon addition of acid or base. Add only a few drops...

  7. Immunocytochemistry

    Type
    Protocol
    ...min at RT in the dark. Pro-Tip The plate can be wrapped in foil to block light. Remove the secondary antibody...

  8. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...mutations, the risk is high. You are generating a gene therapy product with CRISPR to (hopefully) be used in human...for mismatches within the gRNA sequence. Graphic courtesy of Maya Kostman. The most commonly...

  9. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...numerous applications, including transgenesis, gene-trap screens, and gene editing. How does the piggyBac...and M. H. Wilson, “piggyBac-ing models and new therapeutic strategies.,” Trends Biotechnol., vol. 33, no...

  10. Finding Your Perfect Job After University

    Type
    Blog Post
    ...and luckily genetics is a sector which has been rapidly growing. These factors left me optimistic that ...would advise you to be wary of companies which are rapidly expanding, as this might not be sustainable. After...

  12. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    .... I've found cooking, gardening, hiking, and photography to be very satisfying. Finally, many PFs are...distance runner, gardener, cook, and outdoor photographer.  Additional Resources on the Addgene Blog ...

  14. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...block its catalytic activity and instead become trapped in a covalent bond with its substrate. Then, all...proteins in vitro reveals complex events. a) Micrographs of a fluorescently-labeled actin filament (488...

  15. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    ...directly manipulating affect an outcome (age, demographic, diet, etc., in a cancer drug clinical trial,...know type of distribution you are working with, graphing data with a histogram is sometimes sufficient ...

  16. Lentivirus Production

    Type
    Protocol
    ...first. Pro-Tip The pH of this solution will drift rapidly upon addition of acid or base. Add only a few drops...

  19. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ...from making off-target cuts.   Pro tip! X-ray crystallography cannot capture flexible or disordered regions...to manipulate it. Happy CRISPRing!   Molecular graphics and analyses performed with UCSF ChimeraX, developed...
Showing: 261 - 280 of 497 results