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We narrowed to 503 results for: RAP

Showing: 261 - 280 of 503 results
  1. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...known functions has the potential to be used in therapeutics, as it can increase gene expression at certain..., Garnett MJ (2019) Prioritization of cancer therapeutic targets using CRISPR–Cas9 screens. Nature 568...
  2. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...Weaker. Commonly found in pBAD vectors. Good for rapid regulation and low basal expression; however, not...to scientists. Plasmids are oftentimes put to therapeutic uses, and in those cases it's important to identify...
  3. Lentivirus Production

    Type
    Protocol
    ...first. Pro-Tip The pH of this solution will drift rapidly upon addition of acid or base. Add only a few drops...
  4. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ... genes with potentially harmful mutations in therapeutic settings. Silencing of a target gene is achieved...end_module_attribute %}{% module_attribute "wrap_field_tag" is_json="true" %}{% raw %}"div"{% endraw %...
  5. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ... (SpyTag-GFP nanobody) assembled on beads. C) Micrograph of H1.8-GFP nucleosomes collected with MagIC-...of glutaraldehyde crosslinking: representative micrograph (C), particle orientation distribution and Fourier-shell...
  6. Evolution of Lab Techniques

    Type
    Blog Post
    ...labeling it. The label can then be visualized by autoradiography and X-ray film for radioactively labeled probes...commonplace in laboratories. As scientific technologies rapidly progress, some common lab techniques we use now...
  7. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    ...universities are trying to compile more comprehensive demographic information about the postdoc work force, problems...sorted out there can be gaps in knowledge of demographics and the information is often confidential (see...
  8. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...mutations, the risk is high. You are generating a gene therapy product with CRISPR to (hopefully) be used in human...for mismatches within the gRNA sequence. Graphic courtesy of Maya Kostman. The most commonly...
  9. Exploring Applications of the Bioluminescent HiBiT Tag

    Type
    Blog Post
    ...endogenous protein activity and measure responses to therapeutics in real time. Let's take a closer look at several... on the cell surface. EGF treatment resulted in rapid internalization and diminished luminescence signal...
  10. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...numerous applications, including transgenesis, gene-trap screens, and gene editing. How does the piggyBac...and M. H. Wilson, “piggyBac-ing models and new therapeutic strategies.,” Trends Biotechnol., vol. 33, no...
  11. Finding Your Perfect Job After University

    Type
    Blog Post
    ...and luckily genetics is a sector which has been rapidly growing. These factors left me optimistic that ...would advise you to be wary of companies which are rapidly expanding, as this might not be sustainable. After...
  12. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    .... I've found cooking, gardening, hiking, and photography to be very satisfying. Finally, many PFs are...distance runner, gardener, cook, and outdoor photographer.  Additional Resources on the Addgene Blog ...
Showing: 261 - 280 of 503 results