We narrowed to 363 results for: gne

1. Degrading DNA with Cascade-Cas3

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   Blog Post

   ...maximum efficiency of the process. Various labs have designed all-in-one Cascade plasmids — such as the Tomoji...

2. Hot Plasmids and Viral Preps - May 2021

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   Blog Post

   ...the mutated spike protein and a barcode, and is designed so that the SARS-CoV-2 spike RBD is displayed ...

3. 3D Printing Meets CRISPR Cas9

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   Blog Post

   ...check out our hands-on teaching tools that are designed to make the molecular world real for students....

4. Fluorescent Tagging of Endogenous Genes with SapTrap

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   Blog Post

   ... not work with C. elegans, several groups have designed modular toolkits to assist with tagging genomic...

5. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

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   Blog Post

   ...part. For example, you can analyze experiments designed outside CrispyCrunch, or you can input pre-existing...

6. Plasmids 101: 5 factors to help you choose the right cloning method

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   Blog Post

   ...amazing project. You’ve written your protocols, designed your experiments, and prepared your reagents. ...

7. Plasmids 101: Protein Expression

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   Blog Post

   ...for proper protein function. Most DNA constructs designed for mammalian expression utilize viral promoters...

8. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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   Blog Post

   ...bacterial polymerase subunit ω. This construct is designed to activate transcription from the accessory plasmid...

9. Truncated gRNAs for Regulating Gene Expression

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   Blog Post

   ...genetic locus when complexed with an appropriately designed small RNA, or guide RNA (gRNA). The gRNA conventionally...

10. Plasmids 101: Optimizing Plasmid Yields

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    Blog Post

    .... Spiking your media with supplements such as magnesium salts, buffering agents, and/or supplying additional...

11. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

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    Blog Post

    ...of environmental changes that the biosensor is designed to detect resulting in false positives [2, 4]....

12. Anatomy of a Plasmid Page at Addgene

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    Blog Post

    ...is a brief description of what the plasmid was designed and used for in the depositing lab, although you...

13. Genome-wide Screening Using CRISPR

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    Blog Post

    ... the genome (see Fig. 2). Individual gRNAs are designed in silico using publicly available gRNA design...

14. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

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    Blog Post

    ...one mistake can be enough. Reversal drives are designed to overwrite a previous gene drive and thereby...

15. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

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    Blog Post

    ...resistant to disease. They also devise biosensors designed to elucidate the molecular interactions that go...

16. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

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    Blog Post

    ...systems with quantitative precision. There are few designers of biological systems today who are building 10...

17. Lentivirus Production

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    Protocol

    ... Hazardous waste container pH meter Stir plate Magnetic Stir Bar Reagents DMEM high glucose, Corning 10...Heat-inactivated FBS 1x PBS pH 7.4 without calcium or magnesium, Corning 21-040-CV (cations can affect the attachment...

18. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

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    Blog Post

    ...are no exception to this effect -- meticulously designed experiments in labs around the world were put ...

19. Great Results Start with Great Standard Curves

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    Blog Post

    ...often recommend using advanced graphing software designed to plot complex trendlines. If you don’t have ...

20. DNA Purification Without a Kit

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    Blog Post

    ...RNA can be amplified. Compared to a commercial magnetic bead-based kit, this cellulose-based method was...