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  2. Antibodies 101: Conjugation

    Type
    Blog Post
    ...multiplexing and can be used in a variety of assay types. Be sure to check the spectral properties of the... et al., 2007; Stoeckius, et al., 2017). Metal isotopes are useful for mass cytometry (Bendall, et al....

  3. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

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    Blog Post
    ...connected to these 3 stages and contains multiple cell types: fibroblasts, myofibroblasts, neuroendocrine, adipose...drug resistance? Although we’ve seen how multiple types of cells affect tumor progression, enzymes and metabolism...

  6. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...amount of working memory resources. There are three types of cognitive load: intrinsic cognitive load is the...organizing and connecting information and a logic to the types and timing of questions that are asked. Many things...

  7. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...antibodies are antibodies generated against antibody isotypes from a specific species, such as the rabbit IgG...primary antibodies are different species and/or isotypes. For highly multiplexed assays, using secondary...

  8. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    ...member. But, at Addgene, we use seven different types of “plates” (Fig. 1). When you have to tell an engineer... barcoded (this is pretty Addgene specific) The types or purposes of each given container, when applicable...

  9. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...embryonic stem cells, and/or tissue-specific cell types: Cre-lox recombination. What is Cre-lox? The Cre-lox... that are active only in certain cell or tissue types, during certain stages of development, or by making...

  10. Plasmids 101: The Wide World of Natural Plasmids

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    Blog Post
    ...resistance to toxic substances like heavy metals or pesticides, enabling their hosts to survive where others...addition, scientists have engineered entirely new types of plasmids without clear analogues in nature. For...

  11. Mouse Modeling, Part 1: Genetically Engineered Mice

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    Blog Post
    ...then injected into a blastocyst. There are several types of genetically engineered mice: transgenic mice,...in part 2 of this blog series) controls the cell types in which the conditional recombination can occur...

  12. Hot Plasmids and Viral Preps - March 2021

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    Blog Post
    ...turns green. This system can be used in many cell types including endothelial cell-pericyte, neuron-astrocyte...well as additional useful tags, including 3xFLAG epitopes, TEV and BioTag, which can be used in protein ...

  13. Early Career Researcher Toolbox: Finding Relevant Papers

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    Blog Post
    ...a paper-finding-machine. There are four general types of accounts you can follow: Scientists: Following...fields of research they support. Monitoring these types of accounts can help you find papers related to ...

  15. Viral Vectors 101: Chemogenetics

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    Blog Post
    ...studies in neuroscience where specific tissues, cell types, or even subcellular regions of a neuron need to...expression involve tissue specific promoters, AAV serotypes, and the ever-growing variety of recombinase systems...

  16. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...and mechanism of polyadenylation vary across cell types, but polyadenylation generally serves to promote...III), each responsible for transcribing different types of RNA: Polymerase I is responsible for ribosomal...

  17. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    ...Prader–Willi syndrome, which have very different disease phenotypes, are two fascinating examples of epigenetics ... chromosome 15 are inactivated, but different phenotypes result depending upon whether the improperly ...

  19. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ... Viruses can be produced in many different cell types, spanning both prokaryotic and eukaryotic organisms...viruses can be produced in multiple different cell types, including plants, mammals, and insects. In this...

  20. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...depends on helper viruses like adenovirus (Ad) or herpes simplex virus to facilitate efficient replication...collect will depend on the serotype, as some rAAV serotypes are abundantly released into the culture media...
Showing: 261 - 280 of 513 results