We narrowed to 322 results for: phy

1. Better Dyeing Through Chemistry & Small Molecule Fluorophores

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   ...give you the best of both worlds: the excellent photophysics of chemical dyes combined with the ease and ...

2. CRISPR Antimicrobials

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   ...Bacterial Genome Engineering Treating Muscular Dystrophy with CRISPR Gene Editing Using CRISPR to Edit ...

3. Tips for Screening with Yeast Two Hybrid Systems

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   ...that the configuration of your fusion proteins physically blocks the binding sites for the protein partner...

4. Plasmids 101: Protein tags

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   ...proteins can be purified using metal-affinity chromatography, usually using a Ni2+ column. Like other affinity...

5. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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   ...enChIP) using CRISPR. Fujita T, Fujii H. Biochem Biophys Res Commun. 2013 Aug 11. pii: S0006-291X(13)01329...

6. Your Lentiviral Plasmid FAQs Answered

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   ...differences may seem subtle they result in a key physiological difference between lenti- and retroviruses. ...

7. How to Write a Scientific Review Article

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   ...histone deacetylases in B-and T-cell development, physiology and malignancy." Genes & cancer 6.5-6 (2015):...

8. Plasmids 101: Plasmid Incompatibility

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   ...molecules, forming a “handcuff” structure that physically blocks replication machinery when plasmid copy...

9. New CRISPR Tools: Cas7-11 and PASTE

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   ...originally suspected that this new Cas7-11 might be phylogenetically classified as the more complex Class I systems...

10. Twenty Years of Sharing: Addgene's Viral Vector Service

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    ...Janelia Research Campus on the GENIE website.   Bibliography  Ersing, I., Rego, M., Wang, C., Zhang, Y., ...

11. Viral Vectors 101: Preparing Pooled Libraries

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    ...can transduce your cells.  While Addgene uses a physical titer (via ddPCR) to quality control our viral...

12. Viral Vectors 101: Pseudotyping

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    ...Malone KD, Wolf CR, Chu HY, Tortorici MA, Veesler D, Murphy M, Pettie D, King NP, Balazs AB, Bloom JD (2020...

13. CRISPR-based Genome Editing Systems For Editing in E. coli

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    ...editing in Escherichia coli. Acta Biochimica et Biophysica Sinica. https://doi.org/10.1093/abbs/gmab036 ...

14. Antibiotic Resistance: An Old Solution but a New Problem

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    ...the risk to others. Your friends, family, and physicians will thank you! Many thanks to our Guest Blogger...

15. Using Video to Share Your Science: We Share Science

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    ...research abstracts. With journals in medicine, physics, math, and a few other disciplines leading the ...

16. Changing Labor Laws Bring Increased Postdoc Wages

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    ...changes in science. Follow him on Twitter @BiophysicalFrog and Future of Research @FORsymp.   Additional...

17. Getting the Most from Your Lentiviral Transduction

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    ...translated to other lines. Differences in the physiology of different cell types, however, can make this...

18. Tips for Using FRET in Your Experiments

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    ...Periasamy defined FRET as "a distance-dependent physical process by which energy is transferred non-radiatively...

19. Plasmids 101: The Promoter Region – Let's Go!

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    ...consistent expression regardless of cell type or physiology. SV40 General expression mRNA Mammalian expression...

20. Fluorescent Proteins 101: When GFP lets you down

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    ...biosensors, and optogenetic tools engineered from phytochromes." Chemical reviews 117.9 (2017): 6423-6446. ...