We narrowed to 396 results for: promoter
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TypeBlog Post...Program at Harvard Law School. He has continually promoted the cause of increasing data collection about ...
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Plasmids 101: Golden Gate Cloning
TypeBlog Post...orientation. This allows for multiple DNA components (promoters, genes, terminators, etc) to be assembled in the... -
CRISPR Plasmids - Prime Edit
TypeCollection...Insert Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter Selectable...CRISPR Guide . Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top...top Bacteria ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Drosophila ...find a gRNA vector based on expression system, promoter, the type of gRNA (e.g., pegRNA, epegRNA, nicking...plasmid contains Cas9. ID Plasmid Expression System Promoter Guide RNA Type Cloning Enzyme Co-expressed Cas9... -
Recombinases AAV Preps
TypeCollection...information about these molecular tools. Cre AAV ID Name Promoter Fluorophore/Tag Serotype(s) PI 105551 pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40...AAV.TBG.PI.Cre.rBGe TBG none 8 James M. Wilson Dre AAV ID Name Promoter Fluorophore Serotype(s) PI 50363 AAV phSyn1(S)... Syn none 5, rg* Hongkui Zeng Flpo AAV ID Name Promoter Fluorophore Serotype(s) PI 51669 AAV phSyn1(S)...Research Program Light-Inducible Recombinases ID Name Promoter Fluorophore Serotype(s) PI 140135 pAAV-EF1a-iCreV...EF1a none 1, PHPeB Hongkui Zeng VCre AAV ID Name Promoter Fluorophore/Tag Serotype(s) PI 55638 pAAV-EF1a-vCre... -
Cre-Lox and Other Site-Specific Recombinases
TypeCollection...specific times or locations using cell-specific promoters or inducible systems, you can precisely control...fragments and placed under the control of different promoters. Expression of both fragments in the same cell... lox-STOP-lox shRNA constructs, Cre expression promotes shRNA expression. Gene Switch: These constructs...plasmids based on your expression system of choice, promoter, or search by inducible system (e.g., search for...Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional Addgene resources Explore our full...Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional Addgene resources Search our full... -
CRISPR Plasmids - C. elegans
TypeCollection...lower efficiency than NHEJ. ID Plasmid Gene/Insert Promoter PI Publication Activate Catalytically dead dCas9...gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your gene of interest...to your specific locus. ID Plasmid Gene/Insert Promoter PI Publication Empty gRNA Expression Vectors Select...variety of Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed... -
CRISPR Plasmids - dCas9-FokI
TypeCollection...Plasmid Gene/Insert Promoter PI Publication Drosophila ID Plasmid Gene/Insert Promoter PI Publication Yeast...Yeast ID Plasmid Gene/Insert Promoter PI Publication Do you have suggestions for other plasmids that should... -
Overcoming the Challenges of Lentiviral Production
TypeBlog Post...incorporating it into a fourth plasmid. In addition, the promoter of the 5’ long terminal repeat (LTR) is mutated... -
dTAG - You're it!
TypeBlog Post... al. YY1 Is a Structural Regulator of Enhancer-Promoter Loops. Cell. December 14 2017. 171(7):1573-1588... -
Educational Planarian Tools for Teaching Developmental Biology Techniques
TypeBlog Post...plasmid harbors a multi-cloning site flanked by T7 promoters to allow for bidirectional transcription to create... -
Adeno Associated Virus (AAV) for Cell and Gene Therapy
TypeBlog Post...gene expression and the selection of specific promoters may also help AAV vectors evade host immunity.... -
Early Career Researcher Toolbox: Finding Relevant Papers
TypeBlog Post...papers. Twitter Scientists often use Twitter to promote their work, so it’s also a good place to look for... -
Open Resources and Plasmid Tools For Studying C. elegans
TypeBlog Post...gene expression in C.elegans. In this system a promoter controls the expression of a GAL-4 driver that... -
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation
TypeBlog Post...functional role of DNA demethylation at specific promoters by targeted steric blockage of DNA methyltransferase... -
A Look at Addgene's QC Process
TypeBlog Post...they ordered contains the correct inserts, tags, promoters, and other critical functional components. Fun... -
Hot Plasmids - October 2020
TypeBlog Post...express hybrid Cas9-Cas12a gRNAs under a single U6 promoter. The paralog and dual-targeting hybrid gRNA library... -
Progress Towards a PAM-Free CRISPR
TypeBlog Post...surprise since the PAM flexibility could in theory promote off-target binding. The activity of SpRY was validated... -
Science communication: 9 Strategies to Get Your Foot in the Door
TypeBlog Post...discussions. Get your voice out there. Use Twitter to promote your other science communication activities, e.... -
Illuminating Epigenetics with A FRET Based Biosensor
TypeBlog Post...decreases. Hypermethylation of CpG islands in the promoter region of tumor suppressor genes can repress their... -
CRISPR Plasmids - Epigenetics
TypeCollection...Insert Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter Selectable...modification is typically targeted to a specific promoter or enhancer for your gene of interest. These modifications...