We narrowed to 396 results for: promoter
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TypeBlog Post...nuclear localization, binding to the appropriate promoter sequence and activation of gene expression. In...
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Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages
TypeBlog Post...help them navigate the scientific enterprise and promote their voices in driving changes in science. Follow... -
Tips for Titering Your Lentiviral Preps
TypeBlog Post... had multiple integration events. In addition, promoter choice can also impact transgene expression. The... -
The Future of Research Symposium Boston 2015
TypeBlog Post...Program at Harvard Law School. He has continually promoted the cause of increasing data collection about ... -
Plasmids 101: Golden Gate Cloning
TypeBlog Post...orientation. This allows for multiple DNA components (promoters, genes, terminators, etc) to be assembled in the... -
CRISPR Plasmids - C. elegans
TypeCollection...lower efficiency than NHEJ. ID Plasmid Gene/Insert Promoter PI Publication Activate Catalytically dead dCas9...gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your gene of interest...to your specific locus. ID Plasmid Gene/Insert Promoter PI Publication Empty gRNA Expression Vectors Select...variety of Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed... -
CRISPR Plasmids - dCas9-FokI
TypeCollection...Plasmid Gene/Insert Promoter PI Publication Drosophila ID Plasmid Gene/Insert Promoter PI Publication Yeast...Yeast ID Plasmid Gene/Insert Promoter PI Publication Do you have suggestions for other plasmids that should... -
CRISPR Plasmids - Epigenetics
TypeCollection...Gene/Insert Promoter Selectable Marker PI Publication Plant ID Plasmid Gene/Insert Promoter Selectable ...modifiers. Design your gRNA to target a specific promoter or enhancer for your gene of interest. Available... -
CRISPR Plasmids - Purify Genomic Loci
TypeCollection...Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter Selectable...Marker PI Publication Yeast ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Do you have suggestions... -
Validated gRNA Sequences
TypeCollection...CYC1m promoter S. cerevisiae CTAGATATTAAAATGTCTAA 64379 activate S. pyogenes 23977949 Lu CYC1m promoter S.... or repression experiments use targets within promoters. When possible, the categories described on Addgene's... 46917 interfere S. pyogenes 23849981 Qi CYC1m promoter S. cerevisiae ACAGAGCACATGCATGCCAT 64385 activate...activate S. pyogenes 23977949 Lu CYC1m promoter S. cerevisiae ACTAATACTTTCAACATTTT 64387 activate S. pyogenes...pyogenes 23977949 Lu CYC1m promoter S. cerevisiae ATATCGAATTCCTGCAGCCC 64382 activate S. pyogenes 23977949...23977949 Lu CYC1m promoter S. cerevisiae ATATTCTTTCCTTATACATT 64380 activate S. pyogenes 23977949 Lu CYC1m...GTTGAAAGTATTAGTTAAAG 64388 activate S. pyogenes 23977949 Lu CYC1m promoter S. cerevisiae TACATACAGTAGGATCCTA 64381 activate... -
Caltech Systemic Capsids
TypeCollection... window) . Browse Available PHP.eB AAV ID Name Promoter Description Category PI Controls 28306 pAAV-FLEX-tdTomato... #103006) . Browse Available PHP.S AAV ID Name Promoter Description Category PI 28306 pAAV-FLEX-tdTomato...#127847) . Browse Available PHP.V1 AAV ID Name Promoter Description Category PI 104052 pAAV-CAG-DIO-EYFP...#185136) . Browse Available MaCPNS1 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP CAG...#185137) . Browse Available MaCPNS2 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP CAG...#175004) . Browse Available CAP-B10 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP CAG...#175005) . Browse Available CAP-B22 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP CAG... -
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation
TypeBlog Post...functional role of DNA demethylation at specific promoters by targeted steric blockage of DNA methyltransferase... -
A Look at Addgene's QC Process
TypeBlog Post...they ordered contains the correct inserts, tags, promoters, and other critical functional components. Fun... -
Progress Towards a PAM-Free CRISPR
TypeBlog Post...surprise since the PAM flexibility could in theory promote off-target binding. The activity of SpRY was validated... -
Early Career Researcher Toolbox: Finding Relevant Papers
TypeBlog Post...papers. Twitter Scientists often use Twitter to promote their work, so it’s also a good place to look for... -
Adeno Associated Virus (AAV) for Cell and Gene Therapy
TypeBlog Post...gene expression and the selection of specific promoters may also help AAV vectors evade host immunity.... -
Hot Plasmids - October 2020
TypeBlog Post...express hybrid Cas9-Cas12a gRNAs under a single U6 promoter. The paralog and dual-targeting hybrid gRNA library... -
Open Resources and Plasmid Tools For Studying C. elegans
TypeBlog Post...gene expression in C.elegans. In this system a promoter controls the expression of a GAL-4 driver that... -
Overcoming the Challenges of Lentiviral Production
TypeBlog Post...incorporating it into a fourth plasmid. In addition, the promoter of the 5’ long terminal repeat (LTR) is mutated... -
dTAG - You're it!
TypeBlog Post... al. YY1 Is a Structural Regulator of Enhancer-Promoter Loops. Cell. December 14 2017. 171(7):1573-1588...