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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 588 results for: ret

Showing: 261 - 280 of 588 results

  1. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...expression, bacterial expression, lentiviral, retroviral, AAV, RNAi, luciferase, cre/lox, yeast expression...recombination in bacteria) such as lentiviral, retroviral, and AAV plasmids, choose the NEB Stable strain...

  2. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ...particularly effective as it can result in the retention of introns or the removal of exons. Disrupting...microRNA lead to its depletion and Drosha processing retardance. RNA Biol. 2014;11(10):1243-1249. doi:10.1080...

  3. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ...resistance genes to help cells survive their own secretions. Pro tip! Historically, colicin-producing plasmids...Resources References Demanèche, S., Bertolla, F., Buret, F., Nalin, R., Sailland, A., Auriol, P., Vogel,...

  4. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    ...than pre-designed sets, so identical searches can return multiple answers. You might want to take advantage...fragments, or defined split regions. SplitSet will then return the highest-fidelity overhang set it can find for...

  6. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ...access it later. Pro tip Checking the number of retweets, likes, and comments on a tweet about a paper ...papers are connected via citations, in order to return results that are more relevant to your search terms...

  7. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ... body and thereby correct the mutations using retroviral vectors. However, many diseases require techniques...untreatable until 2008. Following a single dose of subretinal administration of rAAV2 carrying the human gene...

  8. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ..., and Martin L. Yarmush. "Polybrene increases retrovirus gene transfer efficiency by enhancing receptor-independent...., et al. "Expansion and fibronectin-enhanced retroviral transduction of primary human T lymphocytes for...

  9. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...making their surrounding media acidic through the secretion of lactic acid (a by-product of cellular metabolism...depending on the cell line so old cells should be retired! Conversely, cells younger than the 3rd – 4th passage...

  10. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...scientists have taken advantage of the natural secretion of biochemicals and proteins through plant roots... Tagging recombinant proteins with a naturally secreted plant peptide allows for easier access and purification...

  11. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...fluorescence falls within the linear window for the theoretical timer shown in Figure 1B, you can use the equation...differentiates human IPSCs into glucose-sensitive insulin-secreting beta-like cells." Nature communications 7 (2016...

  12. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...activators or repressors. These fusion proteins retain the ability to recognize and bind to DNA; when ...occasions truncated gRNAs (≤15nt) have been found to retain some ability to induce Cas9 to make edits (AC, ...

  13. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...standard curve, and most results can be confidently interpreted as integers. Since each sample is partitioned...method you choose, resist the temptation to over interpret your results: Sybr cannot be used to determine...

  14. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...control of biological systems (Wheeler et al., 2016; Duret et al., 2019). But the field has sometimes seen ...plasmid here!   References and resources References Duret, G., Polali, S., Anderson, E. D., Bell, A. M., Tzouanas...

  15. Of Myc and Men

    Type
    Blog Post
    ...studies investigating mechanisms of endoplasmic reticulum localization, Munro and Pelham casually introduced...Pelham HRB (1987) A C-terminal signal prevents secretion of luminal ER proteins. Cell 48:899–907. https...

  20. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ...within the target window, possibly due to longer stretches of duplexed RNA. Using an ADAR2DD catalytic mutant...perform C-to-U edits, the authors note that it retains its adenine deaminase ability. The authors then...
Showing: 261 - 280 of 588 results