We narrowed to 482 results for: vent

1. London Calling - Addgene’s New Europe Office in London

   Type

   Blog Post

   ...than discussing a new scientific discovery or invention, and sharing those ideas with others. This love...

2. In Living Color: The Skinny on In Vivo Imaging Tools

   Type

   Blog Post

   ...spatiotemporal manner, which is not possible with conventional FPs. In addition, PA FPs can improve the achievable...

3. CASTing Off for New Shores in Human Genome Editing

   Type

   Blog Post

   ... that can deliver a healthy gene as a single intervention for a variety of loss-of-function mutants could...

4. Build Your CRISPR Vocabulary

   Type

   Blog Post

   ...require a tracrRNA. sgRNA: “Single-guide RNAs” were invented by synthetic biologists. An sgRNA combines a crRNA...

5. Antibodies 101: Affinity Tags

   Type

   Blog Post

   ...chromatography requires proper folding of the tag, which prevents insoluble fusion proteins from being purified ...

6. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

   Type

   Blog Post

   ...taking for protocol video production, and keeping inventory of our merchandise collection. While most days...

7. Sensing Neuronal Dopamine

   Type

   Blog Post

   ...dopamine and other biosensors in our AAV vector inventory. References Darvesh, Altaf S., et al. "In vivo...

8. What's the Best Way to Elute and Store Your Plasmid DNA?

   Type

   Blog Post

   ...EDTA chelates any divalent cations like Mg2+, preventing the activity of DNase. While this is very important...

9. Fostering communication in remote collaborations

   Type

   Blog Post

   ...This also accelerates the pace of research by circumventing the slower, sequential communication of emailing...

10. Management for Scientists: Giving Feedback

    Type

    Blog Post

    ...let things fester, give feedback as close to an event as possible. If you wait too long, a small correction...

11. The Stingy Scientist: How the Baby Gel Box Was Born

    Type

    Blog Post

    ...biologists used this procedure daily before the advent of techniques like PCR and DNA synthesis. Most ...

12. Plasmids 101: Antibiotic Resistance Genes

    Type

    Blog Post

    ...directly kill bacteria (bactericidal) or slow growth/prevent cell division (bacteriostatic); however, the distinction...

13. Viral Vectors 101: Transductions

    Type

    Blog Post

    ...depending on the virus, cell type, and/or desired event.   Figure 1: Transduction to expression ...

14. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type

    Blog Post

    ...that is directly conjugated to your reporter circumvents the issue. However, directly conjugated primary...

15. What the HEK?

    Type

    Blog Post

    ...short culture periods (less than 20 passages) to prevent these issues from popping up, and if your research...

16. Science Careers: Unruly Interests Feed Many Paths

    Type

    Blog Post

    ...galaxy. TL;DR: Many roads need scientists open for adventure. Many thanks to our guest writer, Pamela J. Hines...

17. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

    Type

    Blog Post

    ...development of monoclonal antibody (mAb) technology circumvented many of these issues by creating immortal antibody-producing...

18. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

    Type

    Blog Post

    ... camelids. They possess many advantages over conventional antibodies: they are small (12-15 kDa), stable...

19. The Golden Stopwatch Award: Recognizing Facilitators of Research

    Type

    Blog Post

    ...Transfer: the exchange of intellectual property (as inventions, ideas, plasmids) among universities and other...

20. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

    Type

    Blog Post

    ...inconsistencies in the work, missing information that would prevent the study to be replicated or omission from the...