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We narrowed to 1,053 results for: FER

Showing: 421 - 450 of 1053 results

  1. Addgene’s Newest Way to Speed Your Research: The Cloning Grade DNA Service

    Type
    Blog Post
    ...preparative experiments.  We’re excited to begin offering this service to further contribute to our mission...and discovery.” Here are the details. Plasmids offered as cloning grade DNA As we begin testing out this...that this service will be helpful to the many different scientific communities using plasmid tools. Details... We will ship you  2 µg of DNA in 20 µl of Tris buffer suitable for molecular biology experiments such... our Plasmids 101 blog series Learn about the different grades of DNA  Read about the various ways to ...other beta projects Read our Molecular Biology Reference ...

  2. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    ...best with diverse teams, to leverage the preferences of different personality types, and to devise strategies...biotech clients. In this realm, there are a few different kinds of challenges that consultants get called...consulting experiences, educational training, and conferences, and synthesizing this knowledge into a coherent...excessively demanding. For instance, a client may offer a lot of pointed feedback or abruptly change needs...consulting firms coach and counsel employees on the different personality and work styles (e.g., Myers-Briggs...prepare you for a successful career in several different fields. While some people continue to enjoy the...

  3. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...example, CRISPR activation has been used to differentiate induced pluripotent stem cells (Chavez et al...neuronal cells by activating genes that cause differentiation and to reverse HIV viral latency (Bialek et...directed towards that gene as there can be a large difference in guide potency. Thus, in cases where users ...cannot be used to study in cis regulation and also suffer from an inability to easily deliver the appropriate...Chavez et al., 2016). To learn more about the different types of Cas9 activators, check out our blog post...DNA sequence or regulating gene expression.     References Behan FM, Iorio F, Picco G, Gonçalves E, Beaver...s41586-019-1103-9 Bialek JK, Dunay GA, Voges M, Schäfer C, Spohn M, Stucka R, Hauber J, Lange UC (2016)...

  4. The Challenges of Cell Culture

    Type
    Blog Post
    ...today. It is now known that nearly 500 cell lines suffer from contamination with HeLa being the most frequent...Down-Regulation of HIF-1α by NDRG2 Expression Attenuates Proliferation and Invasion in Renal Cancer Cells, Marker-independent...the cell lines deposited with them and used by different researchers are free of contamination and match...from this bank. I am often asked, “What is the difference between materials available from commercial repositories...the same cell line from a colleague?”. The main difference is the QC and validation carried out by the commercial...which can be stored in liquid nitrogen long term, preferably some of these vials will be stored in a separate... drug testing. You can find Nick on LinkedIn. References 1. Skloot, Rebecca, and Bahni Turpin. The immortal...

  5. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    ...Cary Valley, and Holly McQueary References and Resources References Niedringhaus, T. P., Milanova, D....identify the bases (and, if necessary, separate different samples), the resulting data, or reads, are essentially...plasmid sequence. However, if there are major differences from the expected sequence, the QC team can manually...theoretical sequences submitted to Addgene have minor differences due to in silico variations and lack of verification...script for manually inputting known innocuous differences. This script compares the sequence for a 100%...Additional Resources on addgene.org Molecular Biology Reference Sequence Analyzer Protocol: Sequence Analysis ...

  6. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...proteins also contribute to this regulation in different ways: methylating newly synthesized DNA, blocking...numbers can allow RepA to link iterons from two different plasmids, forming a pair of “handcuffs” that blocks...RepA proteins form complexes between iterons on different plasmids in a process called "handcuffing," which...Plasmids under stringent control of replication may differ in the specialized proteins or sequences necessary...was updated by Emily P. Bentley in June 2024.  References and resources Further Reading Quiñones-Valles...Replication. IntechOpen. https://doi.org/10.5772/19313 References Asano, K., & Mizobuchi, K. (1998). Copy number...molecular biology help with our Molecular Biology Reference Pages   ...

  7. Guide to Using Pooled Libraries

    Type
    Guide
    ...pooled libraries are single preparations of many different plasmids. Plasmids within a given library have...the same backbone, but they express or target different genes. Some libraries cover the majority of the... library from the Moffat lab has over 175,000 different gRNA-containing plasmids). Amplifying and Using...screen. If you need to amplify the library, please refer to the depositor’s protocol for the best results...conducting pooled library screens. Addgene does not offer next-generation sequencing services. For an NGS ...Genome-Scale, Knockout Screening Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens...

  8. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

    Type
    Blog Post
    ...fragments that are very different.” Because the fragments produced will have different overhangs, they can...enzymes. Now, a new toolkit - aptly named GreenGate – offers all of those same advantages to researchers working...systems. “The cloning is all done in vitro; it’s no different in mouse, human or plants,” explained Jan Lohmann...Lohmann of Heidelberg University. “What is different is the plasmids you use to bring this into your target...available, making it easier for us to do our work.” References 1. Athanasios Lampropoulos et al. "GreenGate ...

  9. March for Science

    Type
    Blog Post
    ... science) they come from many different places supporting many different positions. It would be detrimental... and everyone is welcome. Why? There are many different things scientists and non-scientists alike are...not necessarily marching for every cause. The references provided also only provide a snapshot into the...microbial interactions, and science education.       References March for Science Website Locke, John, and Peter...York Times: With Trump in Charge, Climate Change References Purged from Website The Washington Post: Trump...The scientific community: Diversity makes the difference  Association for Psychological Science: Diversity...

  10. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...purified protein at different acid concentrations. Overviews of the pKa values of different fluorescent protein... GFP variants. Here I use the name ‘GFP’, which refers to mEGFP. This variant was made by introducing ...reduces its size. The substantial size of GFP may interfere with the activity or the localization of the fusion... Another solution to avoid autofluorescence is offered by bioluminescent proteins. These probes generate...C-terminus of another protein. This may, however, interfere with the function of the protein. This is clearly...can follow him on twitter: @joachimgoedhart.   References 1. Zacharias, David A., et al. "Partitioning ...

  11. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    .... The plasmids within it contain a variety of different promoters followed by leader sequences (encoding... at the BIOFAB showed that expression of many different genes was predictable based solely on the promoters...or protease sites to remove the epitope tags. Different plasmids in the kit can be used to purify proteins...prokaryotic expression in recent years, with many different systems now available. However, it can be difficult...ORF clones to allow testing and comparison of different cell-free expression systems; the Cell-free expression...bind specific RNA bases. By swapping out these different modular repeat segments, you can create a PUF ...proteins with a wide variety of functions via different fusions. Some applications of PUF protein fusions...

  12. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ...however different proteins will likely achieve different levels of incorporation due to differences in stability... further technical reference and protocols for use of this system, please reference ‘Rinehart Lab reagents... length of induction, which will vary between different proteins. Beyond these standard considerations...mitochondrion. You can follow her on Twitter @nieminm.   References 1. Cohen, Philip. "The origins of protein phosphorylation...

  13. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...similarities with humans. However, rats have some key differences in physiology, intelligence, and human correspondence... generate transgenic axolotls for an array of different studies. Elly Tanaka’s lab has constructed several...number of offspring (produces many seeds), self fertilizing, a small yet highly characterized genome, and...include understanding of allelic variation between different plant species and metabolic properties affecting... collections to help start your experiments! References: Blount, Z. D. (2015). The unexhausted potential...Zhang, C., & Xing, X.-H. (2018). Pooled CRISPR interference screening enables genome-scale functional genomics...Plasmids Collection Page Read our molecular biology reference Find plasmids for your research ...

  14. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...research coming out of the lab.  References and resources References Hao, Y., Yan, J., Fraser, C., Jiang...integrins at non-ligand-binding sites and do not interfere with integrin function. Overall, the synthetic...then used these antibodies to show the binding preferences of the αV subunit and its β partners. Of interest...contrast, LVDP-binding integrin α4β1 required and conferred less force, leading cells to spread into symmetrical...antibodies and affinity reagents to study the difference in cell morphology changes induced by integrins...discovered by yeast display reveal αV subunit pairing preferences with β subunits (p. 2024.01.26.577394). bioRxiv...

  15. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...this results in the formation of a DNA structure referred to as the displacement loop (D loop). The recombination...when selecting a type of donor. ssDNA templates (referred to as ssODNs (oligodeoxynucleotides)) are commonly... difficult, thus dsDNA plasmids are generally preferred for large insertions such as fluorescent proteins... blog post on Easi-CRISPR for more details. References and resources Want to know more? Check out the...Genome Editing General Homologous Recombination References: Repair of Strand Breaks by Homologous Recombination...their potential to induce chromosomal aberrations. Pfeiffer P, et al. Mutagenesis (2000). PubMed. Multiple...Cas-mediated Transgenesis (2018). Pubmed. CRISPR References: Genome engineering using the CRISPR-Cas9 system...

  16. X-CHIME: Context Dependent Germline Knockout in Immune Cells

    Type
    Blog Post
    ...However, many genes have different functions in different cell types, at different times, and in combination...T cell responses to cancer.     References and resources References LaFleur, M. W., Lemmen, A. M., Streeter...combination with different genes. Having CRISPR systems for assessing genes in defined contexts would be quite...

  17. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

    Type
    Blog Post
    ...shared ownership. Go check it out! References and Resources References Radtke, A. J., Chu, C. J., Yaniv,...mean highly multiplexed: you can image over 65 different parameters using this method (see Movie S8 from...high-quality antibodies and reagents that you know work…preferably before you start your experiment. (And you’re...fluorescent probes, representing imaging from 47 different tissues. Researchers upload and share both their...resources .csv file for easy filtering and sorting. Reference images can be viewed by clicking on the links ...

  18. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    ...tumor-inducing” or Ti plasmid, which allows it to transfer genetic material into the host plant genome. Scientists...Scientists have worked to optimize this system for gene transfer, studying the stability of modified Ti plasmids...borders specify the region of DNA that will be transferred to the plant, similar to viral vectors’ terminal...proportional to size, and many plant scientists want to transfer large genes, smaller binary vectors with a larger..., respectively, making it ideal for large gene transfer. The M24 promoter provides constitutive, high-...foundational studies of promoter and gene expression. References Sahoo, Dipak Kumar, Nrisingha Dey, and Indu Bhushan...

  19. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...from a licensed distributor) allow you to quickly transfer a DNA sequence from the transposon vector to one...sequences or mutations behind. Furthermore, piggyBac™ offers a large cargo-carrying capacity (over 200 kb has...line with the piggyBac™ system is that it has a preference for integration at regions of open chromatin,...rearrangement (such as when a stem cell initiates differentiation), transgene silencing may occur (as it would...cassette can be incorporated, and used during the differentiation process. Moreover, a splinkerette-PCR assay...technologies and strategies for their projects. References 1. L. E. Woodard and M. H. Wilson, “piggyBac-...between inverted loxP sites is cytotoxic for proliferating cells and provides a simple tool for conditional...

  20. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    ...subclass, and are part of the reason why different isotypes drive different types of immune responses. Handily... do so much more than that!    References and resources References Clynes RA, Towers TL, Presta LG, ...immune cells. (psst! Need a quick review of the different immune responses? We got you! You can also review...receptors; and so on and so forth. There are a lot of different receptors, but their downstream effects can be...

  21. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    ...Argonaute proteins play an important role in RNA interference, binding RNA guides to cleave foreign RNA. Argonaute...editing efficiency. Gao et al. tested if NgAgo preferred certain guide sequences - using 5 guides/gene ...gene for 10 genes, they did not observe differences in cleavage efficiency. If this finding is replicated...) or staggered (Cpf1) cleavage. NgAgo takes a different approach, randomly removing 1-20 nucleotides from...Read About NgAgo User's First Impressions Here References 1. Gao, Feng, Xiao Z. Shen, Feng Jiang, Yongqiang... 2. Swarts, Daan C., et al.  “DNA-guided DNA interference by a prokaryotic Argonaute.” Nature. 507(7491...

  22. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    ...redesigning viral packaging plasmids, investigating different viral production strategies or developing novel... already a common excipient in AAV formulation buffers, this sugar may be a more attractive choice for...silver stain approaches we show that there is no difference in purity or infectivity between the untreated...GFP-expressing AAV particles we did not observe any difference between untreated and sucrose treated cells. ...it will be exciting to test this approach with different capsids. Most importantly, because our AAV yields...providing new materials to the research community. References 1. Coroadinha, A. S., et al. "Effect of osmotic...

  23. RUBY-Red Siliques

    Type
    Blog Post
    ...microscopy and beautiful imaging.   References and Resources References  He, Y., Zhang, T., Sun, H. et al...killing the untransformed seedlings or callus (undifferentiated plant cell culture), which is great for a ...such as cacti and beets. Betanin belongs to a different chemical family than the red/purple plant pigments...left) Mature silique (Arabidopsis fruit) with fertilized developing seeds from a homozygous RUBY plant...(Bottom panels) RUBY expression can vary from different transformation events likely due to insertional...

  24. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    ... use of several Cre transgenic mice targeting different brain areas enabled the drawing of a detailed ...the genOway press release for more information. References Abdallah, Khaled, et al. "Adeno-associated virus... delivery of Cre recombinase in mouse primary afferents." Scientific reports 8.1 (2018): 7321. PubMed .... PubMed Central PMCID: PMC3979449. Fenno, Lief, Ofer Yizhar, and Karl Deisseroth. "The development and...and Luk H. Vandenberghe. "Therapeutic AAV gene transfer to the nervous system: a clinical reality." Neuron...comparative study of strains of Halobacterium halobium differing in pigmentation." Biochemical and biophysical ... Addgene website Visit our molecular biology reference Find Cre-lox plasmids at Addgene Browse plasmids...

  25. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    ...saw that these AAV particles come in a slightly different shape. These AAV particles are often found with...able to infect more cells (as demonstrated in different cellular environments such as retina or glioblastoma...purification protocols for isolating exo-AAVs rely on differential centrifugation steps to separate cell culture...Exo-AAVs have improved transduction profiles in different AAV serotypes both in vitro and in vivo when compared...to inactivation through anti-AAV antibodies. References György, Bence, et al. "Naturally enveloped AAV...PMC3345986. Meliani, Amine, et al. "Enhanced liver gene transfer and evasion of preexisting humoral immunity with...

  26. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    ...efficacy of different materials so that we could have discussions about the pros and cons of different fabrics...about the difference between airborne and aerosolised droplet transmission and how different fabrics and...sexual and reproductive health.    References and Resources References Drummond, C., & Fischhoff, B. (2017...

  27. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    ... is it different from a CV? Get familiar with the general format of a resume and the different presentation...Wondering how you can develop more transferable skills? See Developing Transferable Skills During Science Training...advantage over other candidates. Write about your transferable skills Some of the skills you developed might...have presented in some public lectures? These transferable skills could be meaningful for your potential...

  28. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

    Type
    Blog Post
    ...cancer due to its involved in cell proliferation and differentiation.  Since Ras is a membrane localized...gene expression changes lead to cell proliferation and differentiation. The lab confirmed this OptoNB-SOS...OptoNBs that bind cell signaling receptors would offer more levers to pull to control signaling pathways...for more uses both inside and outside cells. References Gil, Agnieszka A., et al. "Optogenetic control...

  29. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    ...work to. Before submitting your paper to your preferred  journal, it is important to check the journal...reviewer’s name and affiliations, and the entire referee report are permanently attached to the published...authors and readers are able to comment on the referee reports. If the reviewers suggest that the manuscript...of the article, while the first version and the referee reports remain available online. Open-peer reviewing...reviewing is beneficial for both the referees (as they get credit for their time and effort) and the scientific...prerequisites of the journal. Ask colleagues (preferably native English speakers) to proofread your manuscript...

  30. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    ...transition from a clump of cells to a fully differentiated body. In contrast, these dynamic processes ...distinguishing cell populations that have been tagged with different fluorescent proteins or dyes. 3. Two-photon microscopy...cells. For example, by harvesting lymph nodes at different times after antigen challenge, scientists were... see movement and interaction between several different immune cells. These methods allowed immunologists... like this is a mosaic of cells labelled with different fluorescent proteins. Using one iteration of this...understanding of the immune system and its dynamics. References 1. Peters, Nathan C., et al. "In vivo imaging...17145958. PubMed Central PMCID: PMC2118178. 6. Schaeffer, Marie, et al. "Dynamic imaging of T cell-parasite...
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