We narrowed to 862 results for: LIC

1. 9 Tips to Achieve Success in Academia

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   Blog Post

   ...analytical and critical thinking skills that are applicable to a variety of careers outside of academia. ...

2. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

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   ... lentiviral libraries and their demonstrated application offer proof-of-principle that CRISPR-Cas9 can...

3. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

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   ...lobe. Image from Isaacman-Beck et al., 2019. Applications for SPARC SPARC technology is versatile and ...

4. Antibodies 101: Fc Effector Functions

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   ...determination, don’t generally affect antibody applications like ELISAs or Western blots, they are key in...

5. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

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   ... experiment to demonstrate the most exciting application for ARG’s: in vivo imaging. Specifically, arg1...

6. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

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   ...c)(3). That means we were formed to benefit the public, not private interests. On this nonprofit awareness...

7. Fluorescent Proteins 101: Fluorescent Biosensors

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   ..., and you should always check the associated publication for the specifics of your chosen plasmid. Types...

8. Management for Scientists: Giving Feedback

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   ...outcome? What help did you need? What help did you solicit? When did you first see you needed help? What...

9. 10 Ways to Share Your Science!

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   ...science and society by connecting scientists to the public. A graduate student I know who participated was...

10. The Stingy Scientist: How the Baby Gel Box Was Born

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    ...glass milk for purifying cut plasmid DNA from agar slices.  Molecular biologists used this procedure daily...

11. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

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    ...antibodies for extracellular and intracellular applications: CDR grafting to stable frameworks and structure-based...

12. Single-cell tracking of lineage and identity with CellTag

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    ...not silenced.    Find CellTag libraries here!  Applications of CellTag The Morris Lab developed CellTag ...

13. Viral Vectors 101: Transductions

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    ...viral preps available for a variety of broadly applicable and popular viral vectors. Check out our viral...

14. Viral Vectors 101: Parts of the AAV Transfer Plasmid

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    ...contains the proteins needed for the virus to replicate,  and the transfer plasmid which contains the ...

15. Antibodies 101: Chimeric Antibodies

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    ...published, knockout validated, and work in your application. But…they are the same isotype and therefore ...

16. Hot Plasmids and Viral Preps - September 2021

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    ...disrupt gene function by targeting start codons, splice acceptor and donor sites, and introducing premature...

17. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

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    ...vaccines are effective or not. This protein both elicits an immune response as the target of vaccines and...

18. RUBY-Red Siliques

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    ...culture), which is great for a wide variety of applications. But some techniques require you to select against...

19. Meet the New Addgene Board Members!

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    ... interference. In the first two months since publication, the lab got over 100 requests for the plasmids...

20. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

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    ...that could be used as a template for downstream applications (Berns, 1972). Using qPCR and restriction enzyme...

21. Multifunctional Neural Fibers: Modulation from Gut to Brain

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    ...interfaces with the entire body, so the obvious next applications are to expand the tool to different brain-organ...

22. The Strength of Story Telling

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    ...: Interviewer: “Tell me about a time you had a conflict at work.” Interviewee: “I did the most work for...

23. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

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    ...AAV yields. Given the wide range of downstream applications scientists rely on our AAV preparations for,...

24. Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation

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    ...have generated robust tools that have practical applications in monitoring the accidental or intentional ...

25. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

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    ...makes them more adaptable to a broad range of applications. Recently, the Spiess lab developed a set of...

26. Plasmids 101: TOPO Cloning

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    ...rejoining supercoiled DNA ends to facilitate replication. The TOPO technique specifically uses Vaccinia...

27. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

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    ... as bacterial conjugation and recombination, replication and topology, and cloning and gene expression...

28. Advice for Moving Into Sales After Your Science Postdoc

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    ...on the marketing or business background of the applicant. However, although this position did look for ...

29. Mentoring for Scientists: I Have a Mentor, What Now?

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    ...If you you want more great advice on mentoring, click below to download the "Mentoring for Scientists"...

30. New and Upcoming Viral Vectors - September 2019

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    ...Christopher Harvey’s lab. According to their publication (Chettih & Harvey, 2019), “this localization ...

31. Visualizing Genomic Loci with CRISPR-Sirius

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    ...has been widely adapted for a wide variety of applications: base editing, CRISPR activation and inhibition...

32. Improving Transduction Efficiency with Exosome AAVs

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    ... This is particularly useful for therapeutic applications in vivo where endogenous anti-AAV antibodies...

33. The MTA at Addgene

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    ...makes it a lot easier for organizations to make policy decisions on research use agreements. The UBMTA...

34. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

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    ...you choose could be based on your downstream application, time, and instrument availability. UV absorbance...

35. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

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    ... format meant that the posters could be viewed publicly and stored in one place if the participants used...

36. Early Career Researcher Toolbox: Free Tools for Science Writing

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    ...saved from Firefox or Chrome browsers with just one click. Papers can be cited in Word, LibreOffice, or Google...

37. Networking Follow-up: I Met a Person. Now What?

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    ...FiercePharma), or sign up to be alerted about their new publications via Pubmed, so you can congratulate them when...

38. Plasmids 101: Gram Negative and Positive Bacteria

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    ...staining. Image credit: Mark Perkins https://www.flickr.com/photos/occbio/7092385559 For more specific...

39. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

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    ...transmembrane domain, followed by a modified form of the cytosolic C-terminal domain of Stargazin including a terminal...

40. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

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    ...screen, it is necessary to identify the gRNAs that elicit the phenotype, and this depends on the method used...

41. What Good Citizenship Can Do for Reproducibility in Science

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    ...can feel like yet another hurdle in front of publication when all they really want to do is get the paper...

42. Trading Spaces - Visiting Scientist Edition

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    ...expect to generate data that will contribute to a publication? A grant? A patent? It is never too early to ...

43. Plasmids 101: NGS Quality Control for Pooled Libraries

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    ...transformed with the library DNA, they grow and replicate, and then their DNA is harvested, much like one...

44. Learning Technical Skills at a Lab Central Workshop

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    ...mostly used to make new libraries as opposed to replicating them like we do at Addgene), going to the workshop...

45. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

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    ...FAP-TAP system works. Find the Plasmids from the Publication Here Light-induced cell ablation with MG-2I-dL5...

46. With Our New Viral Service, We're Taking CRISPR Further!

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    ...achieving the gRNA representation recommended in the publications associated with these libraries. Please note...

47. Four Factors that Differentiate the Stem Cell Field

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    ... have profound impacts on the development of applications with the potential to save lives. While most...

48. 5 Great Apps for Lab Life

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    ...lab notebook. Lab Archives is a multipurpose application that allows you to share your data with colleagues...

49. Management for Scientists: Delegating is Key

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    ...of the overall project gets left undone or is duplicated. With each component of the project completed...

50. Choosing Your Fluorescent Proteins for Multi-Color Imaging

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    ...month, deciding on the best protein for a given application is a challenge. To help keep you abreast of the...