Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 569 results for: CIT

Showing: 281 - 300 of 569 results

  1. Hot Plasmids February 2024

    Type
    Blog Post
    ...cells remain difficult to genetically edit due to toxicity from electroporation or poor cellular entry with...frequency (nearly 100% in some cases) and minimal toxicity, including in clinically-relevant lines such as...

  2. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ...typically requires transducing with a limiting multiplicity of infection (MOI) to ensure only one copy integrates...lentiviral vector titration and in the use of multiplicity of infection (MOI) for predicting gene transfer...

  3. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ...wavelength to stimulate your opsin. Each opsin has an excitation spectrum (use the resources discussed in post...variant you wish to use to determine the ideal excitation). Lasers can be purchased through Laserglow, ...

  4. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    ...these people and bios were likely part of the publicity. Good panels do not waste time on long introductions...on 1-2 panelist for each question in a way that elicits those interesting stories and opinions. Don't let...

  5. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    ...done the research, gathered up your data into an exciting story, and are ready to present your findings ...person seemed confused, bored, or particularly excited about? If you tried tweaking your pitch throughout...

  7. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...aggregation-prone tetrameric fluorescent proteins can be. Cytotoxicity, improper localization, and decreased functionality...Associated Plasmids from Subach et al. Protein Excitation (nm) Emission (nm) Brightness pKa Maturation ...

  8. Transferable Skills: Negotiation

    Type
    Blog Post
    ...Ethiopian?) to where you are going on vacation (New York City or Hawaii?). So how can you approach improving this...starting date with the company so they know I’m excited while still being able to take my vacation? I want...

  9. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...depends on the ability to monitor their signals – electricity! The research tools for studying voltage within...as neuron subtypes, thanks to viral serotype specificity during delivery (more on this later). Chemigenetic...

  11. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ... viral transfer vectors with limited packaging capacity. Additionally, it may not be feasible to express... Addgene's Plasmids 101 eBook And now for some exciting news... You can now download Addgene's new eBook...

  13. MXS Chaining

    Type
    Blog Post
    ...Cellular Structures Construct Fluorophore Excitation/emission maxima Tethering partner Subcellular... mCherry 587nm/ 610 nm human β-Actin Actin 4 Citrine 516nm/ 529nm human α-Tubulin Tubulin Repeatable...

  14. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...where limited expression of Cas9 is required and specificity is a concern, such as knockout generation or ... in vivo studies, little is known about the immunogenicity and stability of the Cas9 protein in the host...

  15. An Introduction to Adenovirus

    Type
    Blog Post
    ... E1 region). The space in the viral particle’s capacity that is made available by this deletion from the...genome, and adenoviral vectors all have a genomic capacity that is much higher than other viral vectors (...

  16. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ...of concentrations but suffers from decreased specificity, especially as it approaches the minimum and ...are more disheartening than seeing a potentially exciting result quashed by a bad standard curve. Take the...

  18. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...Advantages of lentivirus include a large genetic capacity and the ability to transduce both dividing and...for applications that require the large genetic capacity of a lentiviral vector but for which transient...

  19. Hot Plasmids - August 2020

    Type
    Blog Post
    ... the nucleus and modulate transcription of the mCitrine gene. mCherry is constitutively expressed. Cells...calcium indicator jGCaMP7, that are optimized for excitation wavelengths above 1,000 nm and enable two-color...

  20. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    ...culture. Our developers are a diverse group of ethnicities, cultures, genders, educational, and professional...to for each sprint, to ensure we will have the capacity to finish all of the work. Since we work in iterative...
Showing: 281 - 300 of 569 results