We narrowed to 310 results for: Gnas

...particularly interested in the intersection of cell signaling, metabolism, and the mighty mitochondrion. You...

...result can be quantified by the strength of the signal in comparison to a control sample. Southern blotting...

... working with Drosophila Terminators and PolyA Signals FLEx Vectors Resources at Addgene.org Tetracycline...

... the primary antibody and amplify the detection signal.   Analyzing the western blot Once visible, it’...

...lead to high background that obscures the desired signal. We have successfully corrected this by incubating...

...information regarding which regulatory proteins, signaling molecules, mutations, and environmental conditions...

...expressed has threshold effects, such as with signaling molecules or transcription factors, or when the...

... that off-target effects did not overwhelm true signal in these assays (Dempster et al., 2019). Again,...

...referred to as two-sample or independent, but these designations all refer to the same test. On some occasions...

... volume. The consequence is that you can detect signal in thick specimens. See the video below for an ...

...absence of photoactivation resulting in an increased signal to noise ratio. PA-FPs with higher contrast ratios...

...in energy transfer and thus, your specific FRET signal is unaffected  Due to these fundamental differences...

...of thumb, if you can detect a strong fluorescent signal (without antibody manipulation), then expression...

...cells via temporal modulation of canonical Wnt signaling. Proc Natl Acad Sci U S A, 2012. 109(27): p. E1848...

... promoter for heavy chain expression, and an ER signal/leader sequence (L) for translocation of the heavy... promoter for light chain expression, and an ER signal/leader sequence (L) for translocation of the light...

... The Liu lab modified the nuclear localization signals and codon usage BE4 to create BE4max and AncBE4max...

... IT injections are administered in the cisterna magna or lumbar spine. Image and caption courtesy of Challis...

...160437 pUCIDT-attL1-Worm ABeta-attR5 APP Cleavage signal Alzheimer's Nicholas Tolwinski 160541 pGS_101 APOE...

...antibody and conjugate chosen. Signal Amplification The amount of signal needed from a specific antibody...abundant target, it obscures fainter signals. Fluorophores can emit signals that overlap with each other. Choose... Other Affinity Reagents Handling Visualization Signal Amplification Application Overview Quantification...Fc region. This allows for the amplification of signals by having multiple secondary antibodies binding...sdAbs is usually achieved by conjugation of a signaling molecule directly to the sdAb, or by using a secondary... incorporated in the sdAb. sdAbs can have weak signals due to their monovalent nature. Darpins - Darpins...single arm of the Y). They can be conjugated to signaling molecules and are often used in clinical applications...

...classified by the signaling protein that the receptors couple to: Gq-DREADDs Gq-DREADDs signal through the ...Gi-DREADDs Gi-DREADDs signal through the Gαi/o G-protein and inhibit neuronal signaling by inhibiting adenylate...Gs-DREADDs Gs-DREADDs signal through the Gαs G-protein and activate neuronal signaling by increasing intracellular...small molecules that do not affect endogenous signaling, therefore allowing precise control over the cell...then activates G-proteins to modulate downstream signaling. GPCRs have been shown to be involved in a wide...of biological processes, including initiating signaling pathways in inflammation and neurotransmission...couples to β-arrestin to activate noncanonical GPCR signaling independent of G proteins. This DREADD, termed...