Tips for arabidopsis transformation
Type
Blog Post
...and proteins in living plants, to complementing mutant phenotypes. Arabidopsis is amenable to the floral...
We narrowed to 402 results for: Mut
-
-
AAVs CREATed for Gene Delivery to the CNS and PNS
TypeBlog Post...Targeted Evolution. In this technique, a pool of mutant AAV capsid (cap) expressing plasmids is generated... -
Lentiviral Vector Uses and Overview
TypeBlog Post...transfer plasmids. The packaging plasmid carried a mutated HIV-1 provirus that couldn’t package itself due... -
CRISPR Antimicrobials
TypeBlog Post...defective phagemid; Bikard et al. did not observe any mutations at the target site. In a mouse skin colonization... -
Plasmids 101: Protein tags
TypeBlog Post...column runs. The TEV protease itself, with various mutations used to increase its stability activity, can be... -
Plasmids 101: Shuttle Vectors
TypeBlog Post...the researcher, Zeocin has been shown to cause mutations even in cells with the resistance gene, so it ... -
Plasmids 101: Codon usage bias
TypeBlog Post...sometimes (but not always) be noted in in the “mutation” field on the plasmid page (see plasmid 87904 ... -
Transferable Skills Guide: External Collaborations
TypeBlog Post...leverage another organization’s expertise to our mutual benefit. Our partnership with SnapGene, for example... -
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs
TypeBlog Post... This is to avoid the chances of insertional mutagenesis, which is the integration of transgenes into ... -
Lab to Office Culture Shock
TypeBlog Post...everyone has a voice and the group can come to a mutual agreement is an amazing thing to be a part of and... -
Hot Plasmids - August 2020
TypeBlog Post...) and GRAB_NE1m (medium affinity), as well as GRAB_NEmut as a negative control. Find the sensors at Addgene... -
Plasmids 101: Plasmid Incompatibility
TypeBlog Post...plasmid replication control that are not necessarily mutually exclusive. In the first, the replication initiation... -
Transferable Skills Guide: Creativity
TypeBlog Post...Gather the resources you’ll need ahead of time and mute your notifications during. (Boyes, 2019) If you’... -
Viral Vectors 101: Preparing Pooled Libraries
TypeBlog Post...transduction of cells, resulting in cell population mutated by the viral pooled library. These cells can then... -
Viral Production at Addgene
TypeBlog Post...for viral production are propagated in the endA-mutated NEB Stable strain of E. coli. In addition, plasmids... -
Viral Vectors 101: Pseudotyping
TypeBlog Post...2006) Retrograde neuronal tracing with a deletion-mutant rabies virus. Nat Methods 4:47–49 . https://doi.org... -
Bacterial Toxin-antitoxin Systems as Molecular Biology Tools
TypeBlog Post... a GFP cassette, and introduced site-directed mutations, all without introducing unwanted selection markers... -
CRISPR Activation: A Practical Guide
TypeBlog Post...upregulation of essentially any gene by fusing mutants of Cas9 deficient in DNA cutting activity to a ... -
The Challenges of Cell Culture
TypeBlog Post...between cells lines, as this can be dependent on the mutation rate of the cell line, but it is broadly recommended... -
Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs
TypeBlog Post...fluorescent proteins and were re-engineered by mutating cysteine residues and N-glycosylation consensus...