...those that can are primarily third generation constructs. Lentiviral transfer plasmids are not designed...envelope, and transfer vectors, and the resulting construct is packaged into a viral particle, it is not possible...

...available from Addgene's viral service. Retrograde construct from Alla Karpova at Janelia Research Institute...

...neuron. Cell-specific expression of AAV-delivered constructs can be controlled with cell-type specific promoters...common promoters found in chemogenetic receptor constructs and the cell types in which they drive expression...

...using Minicircle DNA vectors encoding the FAST constructs. This proof-of-concept model opens intriguing...

... Addgene's viral service. Engineered systemic constructs from Viviana Gradinaru's lab at Caltech....

...wildtype LTR promoter is no longer present in these constructs. Why do many γ-retroviral plasmids have similar...

...the RNA polymerase. A transcription complex is constructed from the RNA polymerase and several transcription...

...cerevisiae TALENs Gene targeting TALEN kits - Construct a custom TALEN array for genomic...

...new window) of manuscripts that have used these constructs experimentally. Plasmids In addition to EYFP ...

...cycle; VP1, VP2, VP3 - capsid proteins). When constructing an AAV transfer plasmid, the transgene is placed...

...Synthetase Pairs Bacterial Strains Codon Modified Constructs The genetic code for all life is based upon four...

...interested in. Self-replicating - Once you have constructed a plasmid, you can easily make an endless number...

...Using this method, these authors were able to reconstruct a cleared guinea pig cochlea in 3D. The basic...configuration of the instrument used in this reconstruction lay the foundation for all subsequent versions...sheet microscopy with sequential multiple-view reconstruction (5). In order to image large tissue such as...resolution (8,9).  Thus, LSFM is ideal for 3D reconstruction of clear specimens and for in vivo imaging ...development of vertebrate embryos.  The first 3D reconstructions of clear embryos were obtained by using optical...

...cells from 694 embryos over multiple stages and reconstructed the patterns of gene activity and cell divisions...30944477. Farrell, Jeffrey A., et al. "Single-cell reconstruction of developmental trajectories during zebrafish...PMID: 29853675. Keller, Philipp J., et al. "Reconstruction of zebrafish early embryonic development by...

... are not yet fully understood. We attempt to deconstruct and understand this complexity using existing...

...chromosomes via a cut and paste mechanism. Scientists reconstructed a transposase system based on the naturally ...

...Fourier-shell correlation curve (D), and the reconstructed 3D map with 3.1 Å global resolution (E). Figure...

...antibodies fall into two groups. Seven are reconstructions of the original hybridomas as new reproducible...

...28195 tdp43-EGFP construct2 TARDBP GFP CMV ALS Zuoshang Xu 28196 tdp43-EGFP construct3 TARDBP GFP CMV ALS...28197 tdp43-EGFP construct4 TARDBP GFP CMV ALS Zuoshang Xu 28198 tdp43-EGFP construct5 TARDBP GFP CMV ALS...28199 tdp43-EGFP construct6 TARDBP GFP CMV ALS Zuoshang Xu 28200 tdp43-EGFP construct7 TARDBP GFP CMV ALS...28201 tdp43-EGFP construct8 TARDBP GFP CMV ALS Zuoshang Xu 28202 tdp43-EGFP construct9 TARDBP GFP CMV ALS...28203 tdp43-EGFP construct10 TARDBP GFP CMV ALS Zuoshang Xu 28204 tdp43-EGFP construct11 TARDBP GFP CMV ...

...Emission Depletion (STED), and Stochastic Optical Reconstruction Microscopy (STORM). These dyes have specific...