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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 310 results for: construct

Showing: 281 - 300 of 310 results

  4. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...Cas9 Assembly System Kit Mammalian A system for constructing all-in-one expression vectors containing multiple... separate vectors and then assembled into one construct using Golden Gate assembly. Gersbach MuLE System...

  5. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    ...enough molecules have been analyzed to allow for construction of an image, providing detailed protein localization...fluorophore being imaged multiple times in order to reconstruct an image. The best PA-FPs used for super-resolution...

  6. Retrograde AAV viral preps

    Type
    Collection
    ...available from Addgene's viral service. Retrograde construct from Alla Karpova at Janelia Research Institute...

  7. Caltech Systemic Capsids

    Type
    Collection
    ... Addgene's viral service. Engineered systemic constructs from Viviana Gradinaru's lab at Caltech....

  10. Deisseroth INTRSECT Collection

    Type
    Collection
    ...new window) of manuscripts that have used these constructs experimentally. Plasmids In addition to EYFP ...

  11. Chemogenetics Guide

    Type
    Guide
    ...neuron. Cell-specific expression of AAV-delivered constructs can be controlled with cell-type specific promoters...common promoters found in chemogenetic receptor constructs and the cell types in which they drive expression...

  12. Promoters

    Type
    Guide
    ...the RNA polymerase. A transcription complex is constructed from the RNA polymerase and several transcription...

  13. Bacterial Expression Systems

    Type
    Collection
    ...plasmids and kits for efficient assembly of TALEN constructs. The Zinc Finger Consortium page contains a variety...pET-BiFC mVenus (reconstructed) BiFC Dan Mulvihill 39866 pWA PAS-E BAD K-GAFm iRFP (reconstructed) BiFC Vladislav...GFP (reconstructed) BiFC Lynne Regan 168257 168472 pMRBad-C-wtBlc pET11a-N-DiB2 DiB2 (reconstructed) BiFC...interact, the pieces of the FP can get together and reconstruct the full FP. Find plasmids containing FPs and...

  14. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...deliver genetic cargo into cells. Recombinant AAV constructs are designed by replacing the Rep and Cap regions... most commonly used serotype for rAAV vector construction, as it was the first to be well-characterized...host cells. The full-length transgene is later reconstructed within the cell via different mechanisms. One... they form concatemers and splice together, reconstructing the full-length transgene that can then be ...

  15. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...envelope, and transfer plasmids, and that resulting construct is packaged into a viral particle, it is not possible...

  16. Genetic Code Expansion

    Type
    Collection
    ...Synthetase Pairs Bacterial Strains Codon Modified Constructs The genetic code for all life is based upon four...

  17. Lentiviral Vector Guide

    Type
    Guide
    ...envelope, and transfer plasmids, and the resulting construct is packaged into a viral particle, it is not possible...

  18. Molecular Biology Reference

    Type
    Guide
    ...interested in. Self-replicating - Once you have constructed a plasmid, you can easily make an endless number...

  19. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...Using this method, these authors were able to reconstruct a cleared guinea pig cochlea in 3D. The basic...configuration of the instrument used in this reconstruction lay the foundation for all subsequent versions...sheet microscopy with sequential multiple-view reconstruction (5). In order to image large tissue such as...resolution (8,9).  Thus, LSFM is ideal for 3D reconstruction of clear specimens and for in vivo imaging ...development of vertebrate embryos.  The first 3D reconstructions of clear embryos were obtained by using optical...

  20. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type
    Blog Post
    ...cells from 694 embryos over multiple stages and reconstructed the patterns of gene activity and cell divisions...30944477. Farrell, Jeffrey A., et al. "Single-cell reconstruction of developmental trajectories during zebrafish...PMID: 29853675. Keller, Philipp J., et al. "Reconstruction of zebrafish early embryonic development by...
Showing: 281 - 300 of 310 results