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We narrowed to 306 results for: construct

Showing: 281 - 300 of 306 results
  1. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    ...enough molecules have been analyzed to allow for construction of an image, providing detailed protein localization...fluorophore being imaged multiple times in order to reconstruct an image. The best PA-FPs used for super-resolution...
  2. Retrograde AAV viral preps

    Type
    Collection
    ...available from Addgene's viral service. Retrograde construct from Alla Karpova at Janelia Research Institute...
  3. Caltech Systemic Capsids

    Type
    Collection
    ... Addgene's viral service. Engineered systemic constructs from Viviana Gradinaru's lab at Caltech....
  4. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...deliver genetic cargo into cells. Recombinant AAV constructs are designed by replacing the Rep and Cap regions... most commonly used serotype for rAAV vector construction, as it was the first to be well-characterized...host cells. The full-length transgene is later reconstructed within the cell via different mechanisms. One... they form concatemers and splice together, reconstructing the full-length transgene that can then be ...
  5. Deisseroth INTRSECT Collection

    Type
    Collection
    ...new window) of manuscripts that have used these constructs experimentally. Plasmids In addition to EYFP ...
  6. Bacterial Expression Systems

    Type
    Collection
    ...plasmids and kits for efficient assembly of TALEN constructs. The Zinc Finger Consortium page contains a variety...pET-BiFC mVenus (reconstructed) BiFC Dan Mulvihill 39866 pWA PAS-E BAD K-GAFm iRFP (reconstructed) BiFC Vladislav...GFP (reconstructed) BiFC Lynne Regan 168257 168472 pMRBad-C-wtBlc pET11a-N-DiB2 DiB2 (reconstructed) BiFC...interact, the pieces of the FP can get together and reconstruct the full FP. Find plasmids containing FPs and...
  7. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...envelope, and transfer plasmids, and that resulting construct is packaged into a viral particle, it is not possible...
  8. Molecular Biology Reference

    Type
    Guide
    ...interested in. Self-replicate — Once you have constructed a plasmid, you can easily make an endless number...
  9. Lentiviral Vector Guide

    Type
    Guide
    ...envelope, and transfer plasmids, and the resulting construct is packaged into a viral particle, it is not possible...
  10. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...Using this method, these authors were able to reconstruct a cleared guinea pig cochlea in 3D. The basic...configuration of the instrument used in this reconstruction lay the foundation for all subsequent versions...sheet microscopy with sequential multiple-view reconstruction (5). In order to image large tissue such as...resolution (8,9).  Thus, LSFM is ideal for 3D reconstruction of clear specimens and for in vivo imaging ...development of vertebrate embryos.  The first 3D reconstructions of clear embryos were obtained by using optical...
  11. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type
    Blog Post
    ...cells from 694 embryos over multiple stages and reconstructed the patterns of gene activity and cell divisions...30944477. Farrell, Jeffrey A., et al. "Single-cell reconstruction of developmental trajectories during zebrafish...PMID: 29853675. Keller, Philipp J., et al. "Reconstruction of zebrafish early embryonic development by...
  12. E11 Bio PRISM Collection

    Type
    Collection
    ... set of molecular tools for high-throughput reconstruction of individual neurons in brain tissue developed...Plasmids Additional Resources Protein-barcode Reconstruction by Iterative Staining with Molecular annotations...and molecular tools enabling high-throughput reconstruction of individual neurons in brain tissue. PRISM...segmentation of neurons and proofreading of neuron reconstructions. This minimizes the need for human proofreading...circuit mapping and connectomics. Figure 1: Reconstruction of a sparse set of neurons across 10 million...viruses for highly focused circuit mapping and reconstruction. Potential applications could also include ...imaging of barcodes with expansion microscopy and reconstruction of neurons with machine learning. Adapted from...
  13. CRISPR Guide

    Type
    Guide
    ... the HDR pathway can also help. Additionally, constructs with Cas9 fused to proteins that modulate HDR...
  14. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...28195 tdp43-EGFP construct2 TARDBP GFP CMV ALS Zuoshang Xu 28196 tdp43-EGFP construct3 TARDBP GFP CMV ALS...28197 tdp43-EGFP construct4 TARDBP GFP CMV ALS Zuoshang Xu 28198 tdp43-EGFP construct5 TARDBP GFP CMV ALS...28199 tdp43-EGFP construct6 TARDBP GFP CMV ALS Zuoshang Xu 28200 tdp43-EGFP construct7 TARDBP GFP CMV ALS...28201 tdp43-EGFP construct8 TARDBP GFP CMV ALS Zuoshang Xu 28202 tdp43-EGFP construct9 TARDBP GFP CMV ALS...28203 tdp43-EGFP construct10 TARDBP GFP CMV ALS Zuoshang Xu 28204 tdp43-EGFP construct11 TARDBP GFP CMV ...
Showing: 281 - 300 of 306 results