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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 540 results for: try;

Showing: 281 - 300 of 540 results

  1. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Jul 29;7:12266. David Savage Tryptophan Visualize intracellular tryptophan levels and flux Nanosensor detection...green- and orange-emitting proteins Ratiometric Matryoshka biosensors from a nested cassette of green- and...characterization of dedicated sensors for FLIM, for ratiometry and with high affinity. PLoS One. 2015 Apr 14... Applicable over a Wide Concentration Range. Biochemistry. 2015 Aug 18;54(32):5054-62. Martin Webb Phosphate... detection of an immunoregulatory tryptophan influx/kynurenine efflux cycle. PLoS Biol. 2007 Oct;5(10)... Grx1-roCherry. Redox Biol. 2019 Feb;21:101071. Dmitry Bilan Temperature Genetically encoded ratiometric...

  2. Antibody Production

    Type
    Collection
    ...Purity Antibodies are initially titered by spectrophotometry using a NanoDrop spectrophotometer. If needed...your lot, please email [email protected] . Immunocytochemistry (ICC) Cells are fixed and labeled with the...is compared to an untransfected control. Immunohistochemistry (IHC) Tissue sections are fixed, permeabilized...

  3. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...phosphoserine in >36,000 phosphosites by tandem mass spectrometry (Barber et al., 2018). This phosphosite library...human substrates of kinases of interest by mass spectrometry in a technique called serine-oriented library...proteome-wide Serine-Oriented human Peptide library . Biochemistry , 57 (31), 4717–4725. https://doi.org/10.1021...

  4. Molecular Biology Reference

    Type
    Guide
    ...pieces of DNA. This is not extremely useful when trying to sequence an entire plasmid or an organism’s ... and Recombinant DNA Technology Techniques in chemistry enable isolation and purification of cellular .... Commonly used cloning vectors include Gateway entry vectors and TOPO cloning vectors. If you are looking...UCG, AGU,AGC Threonine Thr T ACU, ACC, ACA, ACG Tryptophan Trp W UGG Tyrosine Tyr Y UAU, UAC Valine Val ...

  5. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...other inntags available through Addgene so you can try these incredible new tools yourself! The tags can...their wild-type counterparts - a real boon to those trying to understand physiological interactions and functions...set of 12 promoter-less Gateway® entry clones to be used with other entry clones encoding the ORFs of interest... article through Addgene. Nihongaki et al., Chemistry and Biology. 2015 Feb 19; 22(2):169-74. High...fusion proteins from any ORF available as a standard entry clone. ORFs from an ORFeome Gateway library or any...traditional restriction enzyme cloning to create an Entry vector containing a promoter for constitutive (CMV...expression, or a promoterless construct. Pre-constructed Entry vectors containing Cas9, EGFP, mCherry, iRFP, tdTomato...

  6. Immunology Research Plasmids and Resources

    Type
    Collection
    ... HTR3 HTR3B 5-hydroxytryptamine (serotonin) receptor 3B 5-HT3B HTR3C 5-hydroxytryptamine (serotonin) receptor... HTN3 histatin 3 HIS2, HTN2, HTN5 HTR3A 5-hydroxytryptamine (serotonin) receptor 3A 5-HT-3, 5-HT3A, 5-... receptor 3, family member C - HTR3D 5-hydroxytryptamine (serotonin) receptor 3 family member D MGC119636...MGC119636, MGC119637 HTR3E 5-hydroxytryptamine (serotonin) receptor 3, family member E 5-HT3c1, MGC120035,...factor receptor superfamily, member 14 (herpesvirus entry mediator) ATAR, HVEA, HVEM, LIGHTR, TR2 TNFRSF17...Open Reading Frames (ORFs) in pDONR-223 Gateway® Entry vectors from the labs of William Hahn and David ...

  7. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Destination Vector pENTR4-HaloTag (w876-1) - Gateway Entry Vector pCS2+/C-Halo , pCS2+/N-Halo - Xenopus Expression...Mammalian Expression pENTR4-SNAPf (w878-1) - Gateway Entry Vector pCS2+/C-SNAPf , pCS2+/N-SNAPf - Xenopus Expression...Mammalian Expression pENTR4-CLIPf (w877-2) - Gateway Entry Vector pET51b-His-TEV-CLIP-tag - Bacterial Expression...

  8. Michael J Fox Foundation (MJFF) Plasmid Collection

    Type
    Collection
    ... table below. ID Plasmid Gene/Insert Mutations Industry Return to top MJFF Shared Research Tools Labs ...table below. ID Plasmid Description Gene/Insert Industry PI Return to top...

  9. Ginkgo Bioworks COVID-19 Collection

    Type
    Collection
    ...entity, please see our Plasmid Distribution to Industry page. These plasmids have not been expressed or...(Ctrunc) ID Plasmid Description Mutations Tags Industry Additional Addgene Resources Blog: Synthesized...

  10. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...Other/Trypanosoma cruzi yes, cut S. pyogenes Neo Docampo tdTomato/pTREX-b 68709 Other/Trypanosoma cruzi...Mammalian BbsI yes, activate S. pyogenes Jaenisch piCRg Entry 58904 Mammalian BbsI yes, cut S. pyogenes Huangfu...

  11. All Antibodies

    Type
    Collection
    ...currently assess western blot, immunohistochemistry, and immunocytochemistry. Addgene will continue to work...

  12. Resolute Plasmid Collection

    Type
    Collection
    ... public-private partnership of 13 members from industry and academia financed by the Innovative Medicine...Gateway-compatible pDEST vectors. ID Plasmid Gene/Insert Industry PI Pooled Libraries CRISPR and overexpression ...

  13. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...receptor for SARS-CoV-2, in a single round of viral entry and infection. This approach allows researchers ..., or variant. ID Plasmid Description Mutations Industry PI Due to export control regulations, MERS and...

  14. Promega Plasmid Collection

    Type
    Collection
    ...collection are available to academic, nonprofit, and industry researchers under research use only (RUO) terms...window) on Promega.com. ID Plasmid Description Industry Plasmid Technology Overview This collection includes...

  15. Deisseroth INTRSECT Collection

    Type
    Collection
    ...accumbens medial shell and behavioral switching. Neurochemistry International 129:104482. PubMed (Link opens...hypothalamus-habenula circuit controls aversion. Mol. Psychiatry 24(9):1351-1368. PubMed (Link opens in a new ...

  16. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...pYPQ203 (pMDC32-Ubi1) - Multisite Gateway T-DNA entry plasmid (attR1 & attR2); Zea mays Ubi1 promoter ...backbone, mammalian expression pENTR4-myc-nuc - pEntry vector that adds a C-terminal nuclear localization...

  17. Antibody Guide

    Type
    Guide
    ...select a new antibody using the above process and try again. In some cases, it may be preferable to buy...Includes: Immunofluorescence (IF) Immunohistochemistry (IHC) Immunocytochemistry (ICC) Cell Sorting - used to...immunofluorescence (IF), immunohistochemistry (IHC), and immunocytochemistry (ICC) use labeled antibodies... one protein of interest. Immunohistochemistry (IHC) and immunocytochemistry (ICC) IF can refer to both...both immunohistochemistry and immunocytochemistry when fluorescent signaling molecules (also called dyes...Applications - Cell Sorting Methods Flow cytometry Flow cytometry, used commonly in immunology, allows you...capture, and sort single cells. Includes: Flow cytometry Read on to learn more about the applications common...

  18. Browse

    Type
    Collection
    ...Gene/Insert Vector Type Backbone Mutations Tags Industry PI...
Showing: 281 - 300 of 540 results