...will introduce unwanted variation into your data. Try different types of pipettes and pipetting methods...application correctly is imperative. Picking your chemistry  The two most common qPCR chemistries are hydrolysis...primer/probe actions to a single step. Scorpion chemistry in particular has been reported to work well in...features of several commonly used chemistries)   Chemistry Background noise Probe Allelic specificity...to need to adjust amounts of reagents in your chemistry, unless running a multiplex reaction or on a rapid...cycle machine. Melt Curves An advantage of Sybr chemistry is the ability to run a melt curve to validate...multiplex qPCR when using fluorescent probe-based chemistry - theoretically, up to five or six reactions in...

...activation domain is an incredible tool for researchers trying to activate target genes, the relatively large ...to customize for targeting your gene of interest. Try them today! Ordon J, et al. Plant J. 2016. PubMed...food and beverage industry, the bioethanol industry, and the pharmaceutical industry, depend on the yeast... pull-down methods and identified using mass spectrometry. The Roux Lab’s new and improved version of ...were validated by both Western blotting and immunohistochemistry, and are summarized in the table below: ...

...Purity Antibodies are initially titered by spectrophotometry using a NanoDrop spectrophotometer. If needed...your lot, please email [email protected] . Immunocytochemistry (ICC) Cells are fixed and labeled with the...is compared to an untransfected control. Immunohistochemistry (IHC) Tissue sections are fixed, permeabilized...

... HTR3 HTR3B 5-hydroxytryptamine (serotonin) receptor 3B 5-HT3B HTR3C 5-hydroxytryptamine (serotonin) receptor... HTN3 histatin 3 HIS2, HTN2, HTN5 HTR3A 5-hydroxytryptamine (serotonin) receptor 3A 5-HT-3, 5-HT3A, 5-... receptor 3, family member C - HTR3D 5-hydroxytryptamine (serotonin) receptor 3 family member D MGC119636...MGC119636, MGC119637 HTR3E 5-hydroxytryptamine (serotonin) receptor 3, family member E 5-HT3c1, MGC120035,...factor receptor superfamily, member 14 (herpesvirus entry mediator) ATAR, HVEA, HVEM, LIGHTR, TR2 TNFRSF17...Open Reading Frames (ORFs) in pDONR-223 Gateway® Entry vectors from the labs of William Hahn and David ...

... table below. ID Plasmid Gene/Insert Mutations Industry Return to top MJFF Shared Research Tools Labs ...table below. ID Plasmid Description Gene/Insert Industry PI Return to top...

...Other/Trypanosoma cruzi yes, cut S. pyogenes Neo Docampo tdTomato/pTREX-b 68709 Other/Trypanosoma cruzi...Mammalian BbsI yes, activate S. pyogenes Jaenisch piCRg Entry 58904 Mammalian BbsI yes, cut S. pyogenes Huangfu...

...entity, please see our Plasmid Distribution to Industry page. These plasmids have not been expressed or...(Ctrunc) ID Plasmid Description Mutations Tags Industry Additional Resources Read Addgene's Blog Synthesized...

...currently assess western blot, immunohistochemistry, and immunocytochemistry. Addgene will continue to work...

... public-private partnership of 13 members from industry and academia financed by the Innovative Medicine...Gateway-compatible pDEST vectors. ID Plasmid Gene/Insert Industry PI Pooled Libraries CRISPR and overexpression ...

...receptor for SARS-CoV-2, in a single round of viral entry and infection. This approach allows researchers ..., or variant. ID Plasmid Description Mutations Industry PI Due to export control regulations, MERS and...

...other inntags available through Addgene so you can try these incredible new tools yourself! The tags can...their wild-type counterparts - a real boon to those trying to understand physiological interactions and functions...set of 12 promoter-less Gateway® entry clones to be used with other entry clones encoding the ORFs of interest... article through Addgene. Nihongaki et al., Chemistry and Biology. 2015 Feb 19; 22(2):169-74. High...fusion proteins from any ORF available as a standard entry clone. ORFs from an ORFeome Gateway library or any...traditional restriction enzyme cloning to create an Entry vector containing a promoter for constitutive (CMV...expression, or a promoterless construct. Pre-constructed Entry vectors containing Cas9, EGFP, mCherry, iRFP, tdTomato...

...accumbens medial shell and behavioral switching. Neurochemistry International 129:104482. PubMed (Link opens...hypothalamus-habenula circuit controls aversion. Mol. Psychiatry 24(9):1351-1368. PubMed (Link opens in a new ...

...pYPQ203 (pMDC32-Ubi1) - Multisite Gateway T-DNA entry plasmid (attR1 & attR2); Zea mays Ubi1 promoter ...backbone, mammalian expression pENTR4-myc-nuc - pEntry vector that adds a C-terminal nuclear localization...

...No. 5, November 2011, pp. 313–327 Scholarpedia : Entry by Robert Campbell Spectra Database : Graphing site...2012, Vol. 134 No.18, pp. 7913–7923 Hoi et al. : Chemistry & Biology, October 2013, Vol. 20 No. 10, pp.1296...

...Gene/Insert Vector Type Backbone Mutations Tags Industry PI...

...Plasmid Gene/Insert Vector Type Mutations Tags Industry PI...

...Gene/Insert Vector Type Backbone Mutations Tags Industry PI...

...Plasmodium (malaria), Toxoplasma (toxoplasmosis), Trypanosoma (African sleeping sickness), Cryptosporidium ...

...possible to study the effect of the amount and stoichiometry of enzymes. The pLC97 plasmid allows for quick...

...Available as Cloning Grade DNA ID Plasmid Description Industry PI FAQ What Quality Control analyses are performed...