...Research Foundation Neuroendocrine Tumor Research Foundation The mission of the Neuroendocrine Tumor Research...funding from the Neuroendocrine Tumor Research Foundation Depositor... Depositor Collections Neuroendocrine Tumor Research...treatments for carcinoid, pancreatic, and related neuroendocrine cancers. The NETRF believes that creating and... progress in the diagnosis and treatment of neuroendocrine cancers. As such, NETRF strongly encourages...Title Hans Clevers Hubrecht Institute Modeling Neuroendocrine Tumors Using Adult Stem Cell-Derived Organoids...Institute Finding the Causes of Small Intestinal Neuroendocrine Tumors Ramesh Shivdasani Dana-Farber Cancer...

...targeted neuronal manipulation. Topics may include: How this technology is used for targeted neuronal manipulation...Series AAV Technologies and Methods for Targeted Neuronal Manipulation Meeting Date September 21, 2018 Contact... AAV-based molecular tools to achieve targeted neuronal manipulation (aka intersectional control). The...fit in the big picture of methods for targeted neuronal manipulation? Best practices for using this techonology... is presenting on) is used to achieve targeted neuronal manipulation. Figures, tables, data, or images...communication representing a reference for AAV use in neuronal intersectional control. Through your participation...Adeno-Associated Virus Technologies and Methods for Targeted Neuronal Manipulation. Haery L, Deverman BE, Matho KS, ...

...Ayoubi, R., Southern, K., Laflamme, C., Virk, H., NeuroSGC/YCharOS/EDDU Collaborative Group, & ABIF Consortium...

...expression Tet-pLKO-puro - Tet-inducible lentiviral shRNA expression pMKO.1 puro - Retroviral ...plasmids for drosophila expression pMT-puro - Inducible gene expression under...destination vector for gene expression Puromycin Mammalian pLKO.1 - TRC cloning ...Retroviral shRNA expression pLenti CMV Puro DEST - Lentiviral Gateway destination...Lentiviral shRNA expression Tet-pLKO-puro - Tet-inducible lentiviral shRNA ... expression, multiple selectable markers pMKO.1 puro - Mammalian retroviral shRNA expression...for mammalian gene silencing pMKO.1 puro - Retroviral plasmid for mammalian...

... expression; also expresses GFP. See pMKO.1 puro for Puro resistance. Hahn 63704 pRetroX GFP T2A Cre CMV...pMSCV PIG (Puro IRES GFP empty plasmid) MSCV For cloning and gene expression; select with puromycin or screen... immortalized cells; additional plasmids include Puro resistance and Neo resistance Weinberg 10676 pMKO...CMV/MSV pRXTN is a modified version of pRX-tight Puro encoding NAT instead of PAC Iglehart 20672 MSCV-... for GFP Bartel 32702 pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE MSCV Conditional overexpression plasmid; deletion...

...photoactivatable transcription systems to induce neuronal differentiation. Nat Methods 14:963–966 . https...

...targeting efficiency. SpCas9 and SpCas9n with 2a-Puro and 2a-EGFP are also available. 2. SpCas9 (or SpCas9n...2A-EGFP and single guide RNA 62988 : PX459; SpCas9-2A-Puro and single guide RNA 48873 : PX460; SpCas9n (D10A...) and single guide RNA 62987 : PX462; SpCas9n-2A-Puro (D10A nickase) and single guide RNA 48137 : PX165...2 vector system - lentiCas9-Blast and lentiGuide-Puro (sgRNA) Browse the Addgene GeCKO Library CRISPR ...PX551; Truncated MeCP2 promoter-driven SpCas9; for neuronal expression 60958 : PX552; U6 promoter-driven; ...pAAV-pMecp2-SpCas9-spA) for expressing Cas9 in neurons. SpCas9 guide acceptor: The PX552 plasmid (#60958...-mediated expression of an sgRNA targeting the neuronal-specific gene NeuN. As a control they designed...

...of the ability of a given lncRNA to cis-repress Mauro Calabrese 106253 pLS-mP-Luc Firefly Insertion of...ratiometric imaging David Nelson 212935 pGL4.84(hRlucCP/Puro) RapidResponse(TM) Renilla Vector for investigating...transcription Pete Stecha 212933 pGL4.84(hRlucCP/Puro) Renilla Vector for investigating regions controlling...firefly luciferase Christopher Vakoc 33307 Ubc.Luc.IRES.Puro Firefly Ubc Lentiviral expression of firefly...firefly luciferase Brent Stringer 102320 FUW-RLuc-T2A-PuroR Renilla Ubc Lentiviral Expression of renilla luciferase...luciferase Mark Kay 140328 pLenti-PGK-Venus-Fluc (puro) Firefly hPGK Lentiviral expression of Venus-firefly...

...semi-quantitative immunoblotting. Journal of Neuroscience Methods, 172(2), 250–254. https://doi.org/10.1016...

...inflammation and infection, regeneration, human neurological disorders, microbiota studies, and drug discovery...Zebrafish Tol2 Gateway-compatible Kit for Neurodegenerative Disorders - Nicholas Cole Lab. A kit consisting...production of transgenic zebrafish for studying neurodegenerative disorders. Fluorescent Protein Fusion Construct...resources and developing new lines and technology. European Zebrafish Resource Center (EZRC) - A repository...) for the distribution of zebrafish lines from European researchers. International Zebrafish Society (...

...control of neuronal activity. Table 1. Descriptions of DREADDs and their activity in neurons DREADD Receptor... and outcome in neurons. Table 2. Descriptions of PSAMs and their activity in neurons PSAM Ion Pore Domain...Outcome (in neurons) Reference PSAM4 Gly Varenicline, uPSEM 792, uPSEM 817 Cl - influx Neuronal inhibition...influx Neuronal activation Magnus et al., 2019 PSAM Q79G,Q139G Gly PSEM 22S Cl - influx Neuronal inhibition...influx Neuronal activation Magnus et al., 2011 PSAM L141F,Y115F Gly PSEM 89S Cl - influx Neuronal inhibition...and their activity in neurons LMO Luciferase Opsin Ligand Effect Outcome (in neurons) Reference LMO3 sbGLuc...expressed. However, neuronal control through PSAMs and LMOs is direct, while neuronal control of DREADDs...

... shRNA; neomycin selection; plasmid 21915 has puromycin selection TetR On Wiederschain 41393 pCW57.1 Lentiviral...cloning; selection cassette in format: PGK-rtTA-2A-puro; see article for tagged insert options rtTA On Root...from pCW57.1. rtTA was replaced with tTA, and Puromycin was replaced with Blasticidin selection. Please... interest. tTA Off Sabatini 92099 AAVS1_Puro_Tet3G_3xFLAG_Twin_Strep Bidirectional promoter controls expression....tTA Adenoviral; CMV promoter; see article for neuronal-specific promoter and enhancer options tTA Off...

...specific subsets of neurons, allowing precise spatiotemporal control of these neurons by turning on and ...expressed in all GABAergic neurons. In this case, the subpopulation of GABAergic neurons being activated by the...engineering to measure and manipulate cells (frequently neurons) and their governing biomolecular processes. The...channelrhodopsin, fused to mCherry, is expressed in neurons (red dots). When exposed to light of the correct...cations flow into the cell (yellow dots), and the neuron is activated. Optogenetics tools can be broadly...and nervous system to better map and understand neuronal circuits. Optogenetic tools have also been used...Drosophila. These tools have been instrumental in neurological disorder research, helping scientists to better...

...specific neurons, often utilizing viral expression systems, to activate or deactivate these neurons in a .... When using these opsins as a tool to control neuronal activity, scientists generally attach a fluorescent... table below for the optogenetics plasmids for neuronal excitation and inhibition. For example, search...

..., Schmidt Neurophysiology Tools These AAV encode tools that can be used for neurophysiology experiments...Tetracycline Transactivators Affinity Purification Neurophysiology Cell Ablation Tetracycline Transactivators ...experiments, including neuronal connectivity labeling. ID Name Expression System Activity Serotype PI 51509 AAV...

...Weber 173220 pPBT-peRNA_GG-Puro Mammalian, piggyBac hU6 pegRNA BsmBI No Puromycin Jacob Giehm Mikkelsen 173222...pPBT-PE2-PuroTK-pegRNA_GG Mammalian, piggyBac hU6 pegRNA BsmBI Yes (Cas9 H840A + MMLV RT) PuroTK Jacob Giehm...

...3xFNLDD/pMXs-puro iChIP plasmid, expresses 3xFNLDD in mammalian cells 51240 3xFLAG-dCas9/pMXs-puro Expresses...recognizing a telomere repeat 63590 3xFN-Tel-TAL/pMXs-puro Expresses a 3xFLAG-tagged TAL protein recognizing...repeat. A retroviral expression vector with the puromycin resistance gene 63591 3xFN-Tel-TAL/pMXs-neo Expresses...

...system plasmids for CRISPR-based knock-in of EGFP-2A-PuroR cassette to the C-terminus of endogenous proteins...vector. The PITCh donor plasmid with an EGFP-2A-PuroR cassette, flanked by microhomologous sequences and...deposited PITCh plasmids were tested by fusing EGFP-2A-PuroR cassette to a nucleolar protein, fibrillarin (FBL...pCRIS-PITChv2-FBL - PITCh donor vector for EGFP-2A-PuroR knock-in into human FBL locus Jorgensen Lab SapTrap...

...tropism - AAV.MaCPNS1, AAV.MaCPNS2 Cell type-biased: Neurons - AAV.CAP-Mac, AAV9-X1.1 For marmosets: Broad tropism... tropism - AAV.CAP-B22 Cell type-biased: Neurons - AAV.CAP-B10 Brain vascular cells - AAV.CAP-Mac For ...: Broad tropism - AAV-PHP.eB Cell type-biased: Neurons - AAV.CAP-Mac Brain vascular cells - AAV9-X1.1,...animal Brain vascular cells - AAV9-X1.1, AAV1-X1 Neurons - AAV.CAP-B10, AAV-PHP.N Broad - AAV-PHP.AX with...

...receptors. Chemogenetic receptors are popular in neuroscience, where they can be used to interrogate brain... function and examine the relationship between neuronal signaling and behavior. Depending on the signaling...the opsin to either excite or inhibit the target neuron. For more detailed descriptions of chemogenetic...