We narrowed to 334 results for: uros
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TypeBlog Post...Philosophy at Harvard University) Tony Cijsouw (Neuroscience postdoc at Tufts University) Scientific reproducibility...
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Zebrafish Plasmid Collection
TypeCollection...inflammation and infection, regeneration, human neurological disorders, microbiota studies, and drug discovery...Zebrafish Tol2 Gateway-compatible Kit for Neurodegenerative Disorders - Nicholas Cole Lab. A kit consisting...production of transgenic zebrafish for studying neurodegenerative disorders. Fluorescent Protein Fusion Construct...resources and developing new lines and technology. European Zebrafish Resource Center (EZRC) - A repository...) for the distribution of zebrafish lines from European researchers. International Zebrafish Society (... -
A Guide to Getting Started in Undergrad Research
TypeBlog Post...who opted to join labs in cancer biology or neurodegenerative disease because they had close relatives suffering... -
Antibodies 101: Validation
TypeBlog Post...Torensma R, Turley H, Pulford K, Banham AH (2015) The European antibody network’s practical guide to finding ... -
Sequencing Options for CRISPR Genotyping
TypeBlog Post...PubMed Central PMCID: PMC3773023. 2. Vouillot, Léna, Aurore Thélie, and Nicolas Pollet. "Comparison of T7E1... -
Optogenetics Plasmid Collection
TypeCollection...specific neurons, often utilizing viral expression systems, to activate or deactivate these neurons in a .... When using these opsins as a tool to control neuronal activity, scientists generally attach a fluorescent... table below for the optogenetics plasmids for neuronal excitation and inhibition. For example, search... -
With an Eye Towards the Future, We Look Back at the March for Science
TypeBlog Post...University of Colorado Anschutz Medical Campus in Aurora, CO where she studies the development and regeneration... -
Which Fluorescence Microscopy Technique is Best for Me?
TypeBlog Post...parallelization (7) have allowed for real-time monitoring of neuronal activity across substantial 3D volumes. In addition... -
Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins
TypeBlog Post...genetically encoded calcium indicators for targeted neuronal imaging." Nature methods 12.9 (2015): 852-858.... -
AAV Molecular Tools
TypeCollection..., Schmidt Neurophysiology Tools These AAV encode tools that can be used for neurophysiology experiments...Tetracycline Transactivators Affinity Purification Neurophysiology Cell Ablation Genome Engineering Labeling Overexpression...experiments, including neuronal connectivity labeling. ID Name Expression System Activity Serotype PI 51509 AAV... -
CRISPR Plasmids - Prime Edit
TypeCollection...Weber 173220 pPBT-peRNA_GG-Puro Mammalian, piggyBac hU6 pegRNA BsmBI No Puromycin Jacob Giehm Mikkelsen 173222...pPBT-PE2-PuroTK-pegRNA_GG Mammalian, piggyBac hU6 pegRNA BsmBI Yes (Cas9 H840A + MMLV RT) PuroTK Jacob Giehm... -
CRISPR Plasmids - Tagging
TypeCollection...system plasmids for CRISPR-based knock-in of EGFP-2A-PuroR cassette to the C-terminus of endogenous proteins...vector. The PITCh donor plasmid with an EGFP-2A-PuroR cassette, flanked by microhomologous sequences and...deposited PITCh plasmids were tested by fusing EGFP-2A-PuroR cassette to a nucleolar protein, fibrillarin (FBL...pCRIS-PITChv2-FBL - PITCh donor vector for EGFP-2A-PuroR knock-in into human FBL locus Jorgensen Lab SapTrap... -
Cre-Lox and Other Site-Specific Recombinases
TypeCollection...Collection Optogenetics Plasmid Collection Monosynaptic Neuronal Tracing CRISPR Plasmids and Resources Site-specific...events but can improve specificity in targeted neuronal populations. Read our blog post about how to minimize...Plasmids Advanced Uses of Cre-lox and Flp-FRT — A Neuroscientist’s View Return to top Flp Recombinase Plasmids...blog Advanced Uses of Cre-lox and Flp-FRT — A Neuroscientist’s View Return to top Dre Recombinase Plasmids... -
Antibody Validation for Flow Cytometry
TypeBlog Post...Ayoubi, R., Southern, K., Laflamme, C., Virk, H., NeuroSGC/YCharOS/EDDU Collaborative Group, & ABIF Consortium... -
Caltech Systemic Capsids
TypeCollection...tropism - AAV.MaCPNS1, AAV.MaCPNS2 Cell type-biased: Neurons - AAV.CAP-Mac, AAV9-X1.1 For marmosets: Broad tropism... tropism - AAV.CAP-B22 Cell type-biased: Neurons - AAV.CAP-B10 Brain vascular cells - AAV.CAP-Mac For ...: Broad tropism - AAV-PHP.eB Cell type-biased: Neurons - AAV.CAP-Mac Brain vascular cells - AAV9-X1.1,...animal Brain vascular cells - AAV9-X1.1, AAV1-X1 Neurons - AAV.CAP-B10, AAV-PHP.N Broad - AAV-PHP.AX with... -
Optogenetics + CRISPR, Using Light to Control Genome Editing
TypeBlog Post...photoactivatable transcription systems to induce neuronal differentiation. Nat Methods 14:963–966 . https... -
Chemogenetics Plasmids
TypeCollection...receptors. Chemogenetic receptors are popular in neuroscience, where they can be used to interrogate brain... function and examine the relationship between neuronal signaling and behavior. Depending on the signaling...the opsin to either excite or inhibit the target neuron. For more detailed descriptions of chemogenetic... -
Fluorescent Protein Guide: Subcellular Localization
TypeCollection...Filaments (neuronal) Nefm EGFP Anthony Brown 32909 pEGFP-mNFM Intermediate Filaments (neuronal) Nefm EGFP...Chromatin H2B mRFP Bryan Welm, Zena Werb 21045 pH2B_mCherry_IRES_puro2 Chromatin H2B mCherry Daniel Gerlich 25999...Autophagosome LC3(ATG8) mCherry Maria Harrison 38277 pMXs-puro GFP-p62 Autophagosome Sequestosome-1 EGFP Noboru... -
Resolute Plasmid Collection
TypeCollection...the Innovative Medicine Initiative (IMI) of the European Union. The RESOLUTE mission is to develop data...This Joint Undertaking receives support from the European Union's Horizon 2020 research and innovation programme... Resources Brain Initiative Plasmid Collection NeuroMab Plasmid Collection Addgene Blog: Gateway Cloning... -
Antibody Plasmid Collection
TypeCollection...R-mAbs is the NeuroMab collection — monoclonal antibodies extensively validated for neuroscience research ...research applications from the NeuroMab/Trimmer Lab Recombinant mAb Collection . Once the plasmids are transiently...