We narrowed to 323 results for: virus

1. What's New in CRISPR - December 2019

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   ... aptamer binding protein for packaging into a lentivirus-like particle. Read the paper in Nucleic Acids...

2. X-CHIME: Context Dependent Germline Knockout in Immune Cells

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   ...pXPR_071, a plasmid that leads to higher titer lentivirus preparations than the first generation CHIME ...

3. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

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   ...technology.” GeCKO library for genomic screens Using lentivirus as the delivery vehicle, Neville Sanjana, also...

4. Cellosaurus: A Cell Line Information Database

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   ...their vaccine production, SARS-CoV-2 research, or adenovirus packaging cell lines dataset collections.  Depending...

5. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

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   ...expression. Because calcineurin is required for virulence in pathogenic fungi, it is an attractive drug ...

6. Single-cell tracking of lineage and identity with CellTag

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   ...reprogramming and differentiation Check out our lentivirus guide Sign up to receive our Viral Vectors 101...

7. 25 Work From Home Tips for Scientists

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   ...the COVID-19 pandemic, check out Care For Your Coronavirus Anxiety and Taking Care of Your Behavioral Health...

8. Multiplexed Overexpression of Regulatory Factors (MORF) collection

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   ...Resources on the Addgene Blog Learn More about Lentivirus Figure out How to Get the Most from Your Lentiviral...

9. Screening for Successful Genome Editing with Digital PCR

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   ...endonuclease mediated gene disruption in the HIV provirus. Sci. Rep. 6, 20064 (2016). PubMed PMID: 26829887...

10. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

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    ...you are equipped and authorized to make and use lentivirus. Also, note that due to the size of the backbone...

11. New Tool for Lineage Tracing: The ClonTracer Library

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    ...infection. When using the ClonTracer system, lentiviruses containing these barcodes infect the target ...

12. Plasmids 101: Cre-lox

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    ...been constructed. Additionally, Cre-containing adenovirus (Ad-Cre) or AAV (AAV-pgk-Cre) has been used to...

13. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

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    ... BirA, and 3) one or more gRNAs Two separate lentiviruses delivering 1) a BioTAP-tagged dCas9 and an eGFP...

14. Truncated gRNAs for Regulating Gene Expression

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    ... for Your Experiments Get Ready-to-Use CRISPR Lentivirus ...

15. Adeno-associated virus (AAV) Guide

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    ...deficient and depend on other viruses such as adenovirus or herpes simplex virus to replicate in the host ...Elements Glossary Adeno-Associated Viruses (AAV) are small, non-enveloped viruses containing a small, linear ...genome. Adeno-associated viruses (AAV), however, are small single-stranded DNA viruses that belong to the Parvoviridae...Tropism Adeno Associated Virus (AAV) for Cell and Gene Therapy Adeno-associated Viruses (AAVs) for Genome Editing...Definition Adeno-associated virus (AAV) Wildtype, small, nonenveloped viruses from the Parvoviridae family...replication process where the virus uses the cell machinery to produce new viruses, eventually lysing the cell...available at Addgene. Adeno-Associated Virus versus Adenovirus Adenoviruses (AdV) are part of the Adenoviridae...

16. Gamma-Retroviral Vector Guide

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    ...leukemia virus (MLV), murine sarcoma virus (MSV), and feline leukemia virus (FeLV). Retroviruses are partially...Gibbon Ape Leukemia Virus (GALV) — T and B cell transduction Murine Leukemia Virus (MuLV) and HIV glycoprotein... that a virus can infect and replicate in. Viral vector The modified form of a wild-type virus used to...packaging genes, while lentiviruses also contain accessory genes specific to each virus type. From an experimental...pathogen, like viruses or bacteria, within a host organism, leading to disease. Provirus The genetic material...packaging genes. However, they are different viruses and require different isoforms of these packaging components...to wide infectivity VSV-G (vesicular stomatitis virus G protein) is the most common envelope gene, due...

17. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

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    ...cheat sheet Read our Quick Guide to All Things Lentivirus Resources on Addgene.org Check out this guide...

18. Deciphering the Mysteries of Behavior with Viral Vectors

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    ...floating. In the same paper, the authors used lentivirus to introduce an shRNA to downregulate expression...

19. Lab to Office Culture Shock

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    ...focusing on my technical skills with cloning and lentivirus production, I didn’t really start to recognize...

20. Antibodies 101: Reading a Flow Plot

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    ...Seroogy, C. (2017). Neonatal immune response to rhinovirus A16 has diminished dendritic cell function and...