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We narrowed to 1,053 results for: FER

Showing: 451 - 480 of 1053 results

  1. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ...poised for more offerings. The viral vector team is publishing research, hosting conferences, and are expanding...lauded by another set of stakeholders - the tech transfer community. In a recent law review article we received...step to maximize the services and features we can offer online for Addgenies and to serve external customers... a lot of successful candidates from internal referrals. Our long reaching reputation helps us hire great...practices and benefits and these are making a difference. We serve scientists around the globe and our...

  2. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    ...a property of the fluorescent protein. This can differ between fluorescent proteins in the same spectral...dependence on excitation power is important since different fluorescence imaging strategies use widely varying..., TIRF or spinning disk confocal use entirely different excitation power regimes. Therefore, it is unpredictable...the best way to compare the photostability of different fluorescent proteins? Measuring photostability...excitation power. By repeating these measurements for different fluorescent proteins and by comparing how the ...measurements reported in the literature are performed in different ways. We describe a number of issues with some...

  3. dTAG - You're it!

    Type
    Blog Post
    ...created a generalizable tag-based strategy, which we refer to as the degradation tag (dTAG) system, to co-opt...selectivity, broad utility for degrading targets in different cellular compartments, and in vivo utility. dTAG...expression of these cereblon neo-substrates varies in different contexts (for example, IZKF1 and IZKF3 are cell-type...technology. Follow him on twitter @behnamnabet.   References Nabet et al. The dTAG system for immediate and... 14(5): 431-441. PubMed PMID: 29581585. This reference contains the full description of the dTAG system... Huang et al. MELK is not necessary for the proliferation of basal-like breast cancer cells. eLife. September...

  4. Summer SciComm: Preprints

    Type
    Blog Post
    ... These each have different goals, formats, and processes, and they provide different amounts of access...research during his PhD studies.  References and Additional Resources References ASAPbio, Preprint Resource ...like publishing a research article, presenting a conference poster or talk, or submitting patent or grant...Going to present some unpublished results at a conference? Post your working manuscript as a preprint too...

  5. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...sources. Mode of communication Different modes of communication take up different amounts of mental bandwidth...definition of cognitive load states: “cognitive load refers to the used amount of working memory resources....with a specific topic; extraneous cognitive load refers to the way information or tasks are presented to... to a learner; and germane cognitive load refers to the work put into creating a permanent store of knowledge...clearly as you intended (it happens to us all!) and offer a variety of options to make your content more accessible...

  6. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ... hand and use them to easily test different antibodies or different signaling molecules. Many labs find...antibodies to bind to a primary antibody without interfering with the hypervariable region, where an antibody...are readily available conjugated to a number of different signaling molecules. With the direct approach,...possible, or ensure that the primary antibodies are different species and/or isotypes. For highly multiplexed...generated in goat against rabbit immunoglobulins. References and Resources More resources on Addgene’s blog...

  7. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type
    Blog Post
    ...scientific manuscript will require numerous other references to scientific literature to substantiate the ...literature related to the topic. Finding reliable references and sorting out what they mean is no small task...Did they perform all of the appropriate controls? Refer back to the methods section for finer points of ...inspiration and accountability. There are a few different ways to automate the process as well. Sign up ...with scribbled notations in the margins. If you prefer, printing and hand-writing notes is still possible... Check out Papers and Mendeley – these programs offer more options for searching and sorting your own ...

  8. Working with Nuclear Receptors

    Type
    Blog Post
    ...such as p300 and CBP histone acetyltransferases or CARM1 methyltransferase. This attendant signaling feature...years evolved from chloramphenicol acetyltransferase to luciferase, the basic assay remains widely used...distinct complexes may explain how more than 300 different coactivator proteins individually contribute to...patterns of nuclear receptors across multiple different tissues in normal and disease states, their circadian...

  9. Lab Automation at Addgene

    Type
    Blog Post
    ... one. As a starting point, I would recommend conferences like Lab Robotic Interest Group (LRIG). There...customizability. It has a large deck that can house many different types of devices. As long as a device fits on ... - one pod can pick up 96 tips for easy sample transfer between 96 well plates. The second pod can pick...out your samples. This can be useful if you’re transferring samples from 96 wells plates onto agar q-trays...follows all the same steps and uses the similar buffers as would be used for a mini prep, except it does...video library Check out our molecular biology reference pages ...

  10. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...plasmids for Borrelia burgdorferi from Christine Jacobs-Wagner’s lab produce different levels of dCas9 expression...system developed to track cell-cell interactions By Jennifer Tsang GFP-based Touching Nexus (G-baToN) is a ...cell surface. When the cells interact, GFP is transferred to the receiver cell, and the receiver cell turns.../genetics/iyab006       The CRISPR corner By Jennifer Tsang Here are a few highlights from recent CRISPR...expression and contain different antibiotic resistance markers. New CRISPR pooled library from Keith Joung...

  11. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ...some of Cas9’s shortcomings. Cpf1’s preferred PAM is 5’-TTN, differing from that of Cas9 (3’-NGG) in both...pattern opens up the possibility of directional gene transfer, analogous to traditional restriction enzyme cloning... cloning. Sticky-end mediated gene transfer would be particularly helpful for targeting non-dividing cells...immunogenicity and range of serotypes allowing preferential infection of certain tissues. However, these...Cpf1 fits into the genome editing landscape. References 1. Kim D, Kim J, Hur JK, Been KW, Yoon SH, Kim...

  12. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ... Snapgene and Geneious software. References and resources References Chadeuf G, Ciron C, Moullier P, Salvetti...sequencing reads (~150 bp each) are aligned to the reference sequence of the plasmid used to create the viral...more than 90% of the reads should align to this reference sequence. Second, we perform a megaBLAST search...search on all the reads that did not map to the reference sequence. The resulting list of hits is then carefully...reads into longer DNA contigs without using a reference sequence. The five contigs with the most total...

  13. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type
    Blog Post
    ... the protein within a living cell. Analyzing differences in protein localization and transport between...signals not visible to the human eye and are indifferent to subjective color intensity perception. Open...statistics that can be used to compare between different experimental setups. A colocalization analysis...one fluorescent signal in a channel setup for a different fluorescent signal) and photobleaching i.e. FP...molecules more closely is Förster Resonance Energy Transfer, FRET. Additional opportunities: Tracking intracellular...Markers of Subcellular Localization Poster here! References 1. Allen Institute Plasmid Pages 2. Cooper GM...

  14. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...but timer activity can be calibrated to these different conditions, enabling the use of a given timer ... of Neurog3 (a gene controlling pancreatic differentiation) in mouse embryos (Miyatsuka et al.) Distinguish... Let us know in the comments section below! References 1. Terskikh, Alexey, et al. ""Fluorescent timer...11090358. 2. Tsuboi, Takashi, et al. "Age-dependent preferential dense-core vesicle exocytosis in neuroendocrine...and Michael S. German. "Chronology of islet differentiation revealed by temporal cell labeling." Diabetes...programmable synthetic lineage-control network that differentiates human IPSCs into glucose-sensitive insulin-...

  15. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    ...chromoprotein and how is it different from fluorescent proteins? Before going into the difference between chromoproteins...research Read our Molecular Biology Reference Find plasmids for FRET References Blanquet RS, Phelan MA (1987...imaging microscopy-based Förster resonance energy transfer (FLIM-FRET) in living cells (Figure 4). They created... Jennifer Tsang...

  16. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...subtypes of A–G, which come from different species and will have different compositions of Cascade. Although...degradative power in the future! References and Resources References Loeff, L., Brouns, S. J., & Joo, ... archaea. Class 1 is further broken down into different types, with Cas3 systems making up type I, the...effector. Type I has subtypes A through G, with different compositions of the Cascade complex. Created with...

  17. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ...focus on gene editing techniques. References and resources References Akhtar, W., De Jong, J., Pindyurin...means we could design hundreds to thousands of different pegRNAs for each desired edit, it simply becomes...many more. Once we had our datasets with all the different features and editing efficiencies, we could train...offline via GitHub. The model provides users with different designs of pegRNAs, ranked by their PRIDICT/PRIDICT2.0... predictive power is sometimes limited across different targets due to variations in chromatin context...

  18. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ...we used different categories (Figure 2C). Note that in Figure 2, the numbers on the X axis refer to distribution...previous Addgene CRISPR publications.  References and resources References Pyhtila, B., Kasowitz, S., Leeson...through the years.  Libraries are divided into different categories based on function: knockout (KO), activate...have CRISPR plasmids deposited from labs in ~40 different countries, and we’ve shipped CRISPR plasmids to...

  19. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

    Type
    Blog Post
    ...rely on two-step hierarchical approaches using different Type IIS restriction enzyme specificities at each...Factors such as enzyme efficiency, stability, and buffer compatibility have placed practical limits on single...Assembly test systems Our research focused on three different levels of assembly: High efficiency and accurate...biotechnological cloning efforts for over 50 years, prefers ligation of Watson-Crick base pair substrates, ...products to market, New England Biolabs, Inc.       References 1. Vladimir, Potapov, et al. "Optimization of...cloning protocols Check out our molecular biology reference pages ...

  20. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    ...be present in a single lentiviral transfer vector or separate transfer vectors. May contain reporter gene...outcomes can be most effectively achieved with different CRISPR components. Select the appropriate CRISPR...4.5kb). CRISPR elements are inserted into an AAV transfer vector and used to generate AAV particles. Transient...cell lines is quite broad and includes several different methods, so we've summarized some key features...should review the current literature about their preferred model.  If your expression system is not well ...

  21. MXS Chaining

    Type
    Blog Post
    ... a nutshell, is the systematic combination of different genetic sequences into plasmid DNA. In high throughput... specific sequences of the genetic elements may differ (e.g., a set of various mammalian promoters), the...cloning can be used to combine the functions of different genetic elements to generate non-natural tools...assembling these modular types of constructs, and different methods are suited for particular downstream applications...Cloning Strategies Method Technology Pros Cons References Golden Gate Type II restriction enzymes Can...Kits at Addgene Find Plasmid Cloning Protocols References 1. Anderson JC, Dueber JE, Leguia M, Wu GC, Goler...

  22. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...known quantities of DNA. Additionally, an AAV reference, a virus with a known titer, can be used to confirm...both results would be valid. This is why the AAV reference is critical. It allows you to determine if your... PCR (ddPCR) does not require a standard or a reference, meaning saving on reagents (and time)! AAV titering...million.  After making the dilutions, they are transferred to a new plate containing the mastermix which...generator, you will have to be careful about transferring your droplets from the droplet generator to ...becoming a popular option for PCR experiments.  References Furuta-Hanawa, Birei, Teruhide Yamaguchi, and...

  23. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    ... as fixing and permeabilizing.  References and resources References Brown-Harding H (2020) Fixation artifacts...Formalin: You may also come across formalin, which refers to a saturated solution of formaldehyde (saturated...100, Tween-20, or saponin. Each detergent has different chemical properties that will impact the level...concentration.      Figure 2: Examples of different permeabilization strategies and when to use them...fixation; they require an additional step generally referred to as Antigen Retrieval. I chose not to get into...

  24. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...the integrity of different targets and labels. You may want to check out a few different protocols to look...you’ve been looking for too!     References and resources References Bertiaux E, Balestra AC, Bournonville... two points within ~250 nm of each other. For reference, individual presynaptic terminals, which are jam...2020). Plus, it’s been demonstrated to work in different model systems, paired with SRM techniques, and...

  25. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    ...Genomic Tools) Program and I met scientists from 21 different labs who clearly also share this wonder of the...convergent evolution because electric organs arose in different branches of the evolutionary tree at least 6-7...cell line, then take the nucleus and put it in a fertilized egg to get edited embryos (replacing the nucleus...editing in the brain allows the squid to create a different protein. Did you know that most cephalopods die...behavior. For example, two similar species have very different social behavior. One is monogamous and one is ...new tools being developed and shared for many different organisms. Do you have a favorite organism you...

  26. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ...critically important to be able to conveniently reference specific pieces of data stored externally in the...of research compiled in the ELN. Without such references the record of research is incomplete, making ...research Just as researchers need to access and reference external data sources, they also need to continue...data from the ELN directly into at least one and preferably more than one of these five general purpose repositories...spreadsheets, images, sequencing data, etc.) used and/or referenced in the experiment. Since most ELNs, unlike data...produced, collected, and analyzed. While the many different ELNs out there can help with searchability, record-keeping...

  27. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...Synthego has developed a new tool called ICE (Inference of CRISPR Edits) to solve this problem. This tool...efficiency and determine the profiles of all the different types of edits that are present and their relative...Contributions   The Contributions tab shows the inferred sequences present in your edited population and... an Indel plot (left panel) which displays the inferred distribution of indel sizes in the entire edited...average amount of signal that disagrees with the reference sequence derived from the control trace file. ...cut site, representing a high level of sequence difference. Traces The Traces tab shows the edited and ...

  28. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

    Type
    Blog Post
    ...colleagues reported that it is possible to reprogram differentiated cells into induced pluripotent stem cells (...original genome by integrating viral vectors are preferred. This is to avoid the chances of insertional mutagenesis...integration-free, karyotypically normal and are able to differentiate into various cell types of all three germ layers...https://www.linkedin.com/in/kmukherjeephd/.     References 1. El Hokayem, Jimmy, Holly N. Cukier, and Derek..., S., et al., LMO2-associated clonal T cell proliferation in two patients after gene therapy for SCID-...Interview with Darrell Kotton Four Factors that Differentiate the Stem Cell Field Sign Up to Write for the...

  29. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ...CRISPR-associated transposition.     References and Resources References: Jiang Y, Chen B, Duan C, Sun B, ...concatenations into crRNA arrays targeting multiple different sites in E. coli. pCutamp cures the plasmids by...MUCICAT toolkit includes versions of plasmids with different induction systems, such as PT7, Prha, Ptet, and...integration frequency. (C) Increasing rounds of transfer on transposition-inducing agar lead to higher ...Capability to construct a strain library with different gene dosages to facilitate swift optimization ...

  30. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ...) enzymes. Various other proteins included in different CGBE tools allow the researcher to direct the ...a translesion synthesis polymerase known to preferentially incorporate A bases across from abasic sites...recognize thymine (T) or cytosine (C). Several different teams succeeded with this approach around the ... gCBE is a deaminase-free C → G editor with a different editing window than existing CGBEs. This distinct...what tools the field will come up with next! References and Resources Glossary Here’s a quick refresher...respectively, to be replaced with another base. References Chen, L., Hong, M., Luan, C., Gao, H., Ru, G....
Showing: 451 - 480 of 1053 results