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We narrowed to 753 results for: ELP

Showing: 601 - 640 of 753 results

  4. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...targeting guide and the transposon RNA homology both help direct the fusion construct to the new genomic locus...

  13. Typing CRISPR Systems

    Type
    Blog Post
    ...general principles of this classification system will help you gain a deeper appreciation and understanding...

  19. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...about depositing your new plasmids with Addgene to help expand the collection! The Jacks lab has made several...

  20. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...without the use of a helper virus. Instead, specific gene products can be provided by helper plasmids (e.g.,...transfer plasmid). Helper Plasmid ID Plasmid Serotype Description PI 112867 pAdDeltaF6 All Helper plasmid for...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...Traditionally, AAV requires the presence of another "helper" virus, such as adenovirus or herpes virus, in ...replication. This requirement has been circumvented with “helper-virus free systems,” which enable the production...., pHelper) and specific packaging cell lines (e.g., HEK293 cells) during AAV production. Read our AAV...In order to generate infectious AAV particles, a helper plasmid (encoding adenovirus E4, E2A and VA) and...

  21. AAV Packaged on Request

    Type
    Collection
    ...unshared. Help Center Check out Addgene’s Help Center, a searchable database of information to help you every...conditions of the applicable transfer plasmid. We will help you gain legal usage of the transfer plasmid from... more reproducible for scientists everywhere and help build a globally connected scientific community....distribution. Troubleshooting If your answer isn’t in the Help Center or you need more assistance with troubleshooting...troubleshooting, we want to hear from you! Email us at help@addgene.org ....

  22. Antibodies

    Type
    Collection
    ...applications. View Guide Need Some Help? Browse some of our other resources to help you choose, store, or use ...distributes ready-to-use recombinant antibodies to help facilitate scientific research. We are building ... developed a variety of educational resources to help you choose and use antibodies, with an expert technical...Antibody Blog Posts Recipient Instructions Addgene Help Center Antibody Protocols Looking for Antibody Plasmids...

  23. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...between the transfer and the helper plasmid, and by providing the helper E1 gene in trans within the packaging...for the production and assembly of viral capsids. Helper plasmid — containing adenoviral genes not present...three production plasmids (transfer, packaging, and helper plasmid) are transfected into cells which then ...rAAV vectors. The E1 gene is not included in the helper plasmid and instead provided by the chosen cell...present as linear DNA molecules. In the absence of helper viruses, wild-type AAVs cannot enter the lytic .... The adenovirus E1A and E1B19K genes provide a helper function for transfection-based adeno-associated...outer capsid shell that protects the viral genome. Helper plasmid E4 in trans Adenoviral early gene that ...

  24. All Antibodies

    Type
    Collection
    ...our Suggest an Antibody form, or email help@addgene.org to help us improve our resources! Acknowledgments...Institutes of Health. Need Some Help? Browse some of our other resources to help you choose, store, or use ...Recipient Instructions Antibody Blog Posts Addgene Help Center...

  25. Antibody Production

    Type
    Collection
    ...shown in your cart. Need Some Help? Browse some of our other resources to help you choose, store, or use ... on the antibody's catalog page or by contacting help@addgene.org . Some of the antibodies distributed...experiments were performed on your lot, please email help@addgene.org . Immunocytochemistry (ICC) Cells are...Recipient Instructions Antibody Blog Posts Addgene Help Center...

  26. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...plasmid available, but helps to inform us of which plasmids would be most helpful to requesting scientists...our Addgene Protocols for resources you may find helpful. Our Scientific and Customer Support Teams are ...are also available to provide assistance at help@addgene.org . Don’t See What You’re Looking For? At this...

  27. mTOR Pathway

    Type
    Collection
    ...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...The content for this page was generated with the help of David Sabatini . mTOR Pathway Color is used for...The content for this page was generated with the help of David Sabatini . Return to top mTOR Pathway -...

  28. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...Engineering of the PE enzyme and related components have helped to improve the efficiency of editing, including...tools, check out our prime editing blog post . Need help designing a pegRNA? Try the DeepPrime (Link opens...

  29. AAVED

    Type
    Collection
    ... experiments). What resources might be useful to help improve this technology or its application? What...antibodies, access to reagents)? For All Attendees: To help us best prepare and compile resources for this meeting's...co-author on this communication and you will be helping scientists successfully adopt these tools. Thank... the meeting preparation section for how to best help contribute to this publication. Citation information...

  30. Ras Pathway

    Type
    Collection
    ...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...content and map for this page were generated with the help of Dominic Esposito and the Frederick National Laboratory...

  31. Adenovirus Guide

    Type
    Guide
    ...vectors requires using an additional adenoviral helper virus (HV) to provide the deleted adenoviral genes...HVs. Figure 4: Third-generation rAdV vectors and helper virus. Created with BioRender.com. Adenoviral Vector...J., Jeong, K. S., & Seol, D. W. (2019). No more helper adenovirus: production of gutless adenovirus (GLAd...window) Rosewell, A., Vetrini, F., & Ng, P. (2011). Helper-dependent adenoviral vectors . Journal of Genetic...that is required for viral genome packaging. In helper viruses, this signal is flanked by loxP sites so...

  32. Neurodegeneration Research Collection

    Type
    Collection
    ...assembled the following to help you find information and plasmid collections that may help with your neurodegeneration... rather than focusing on separate diseases, will help speed progress towards curing and treating them.... website and information resource dedicated to helping researchers accelerate discovery and advance development...

  33. Caltech Systemic Capsids

    Type
    Collection
    ...preparation. Systemic Delivery AAV Capsid Choice Need help deciding which capsid to choose? The following decision...developed at the CLOVER Center at Caltech and can help guide your decision. Figure 1: Decision tree for...service, but lets us know what viruses would be helpful to the scientific community. Text Description for...Decision Tree The AAV decision tree graphic above helps you choose the best capsid for your cell type. First...

  34. CRISPR Guide

    Type
    Guide
    ...introduce the various applications of CRISPR, and help you get started using CRISPR in your own research...experiment. There are many online tools available to help you select an optimized gRNA for your application...naturally-occurring Acr (Anti-CRISPR) proteins can help control CRISPR activity. Acr proteins have evolved...Synchronizing cells or modulating the cell cycle can help as HDR takes place during the S and G2 phases. Chemically...or upregulating those in the HDR pathway can also help. Additionally, constructs with Cas9 fused to proteins...modified scaffold gRNA and additional RNA-binding helper activators; highest levels of single-gene activation...

  35. Plasmids for Stem Cell Research

    Type
    Collection
    ...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...different reprogramming methods with the goal of helping readers choose a strategy suited to their research...expression of key factors. The tables below will help you find plasmids available from Addgene that can...

  36. CRISPR Plasmids - Purify Genomic Loci

    Type
    Collection
    ...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...or search the tables below for CRISPR plasmids to help purify genomic loci. Plasmids are available for ...

  37. CRISPR Plasmids - Parasites

    Type
    Collection
    ...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...The content for this page was generated with the help of Scott Lindner . Cut Fully functional CRISPR/Cas...

  38. Plasmid Collections

    Type
    Collection
    ...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!..., kits, and depositor collections on one page to help you find what you're looking for. If you are looking...

  39. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...your plasmids added to the list. You can also email help@addgene.org for more information and assistance ...

  40. Synthetic Biology - Overview

    Type
    Collection
    ...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...the sharing of parts and information. Addgene can help you to share your synthetic biology plasmids with...
Showing: 601 - 640 of 753 results