We narrowed to 862 results for: LIC

1. Finding and Joining Your Dream Lab

   Type

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   ...does one stand out amongst a pool of successful applicants and get chosen to work in his or her lab of choice...

2. A Quick Guide to a Career in Software Product Management

   Type

   Blog Post

   ... to solve is clear, but the solution is more complicated. You’ll need to be creative to be able to work...

3. Behind the scenes: Addgene’s new search engine and more

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   ... higher than matches of the same name in the publication field. Suggestions We provide as-you-type suggestions...

4. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

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   ...accounting from Babson College. He is a certified public accountant (CPA), certified information systems...

5. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

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   ..., the laboratory of long-time Addgene depositor Alice Ting used yeast display-based directed evolution...

6. Flow Cytometry Readouts: Yes, No, and Everything in Between

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   ...place your gate based on the dot plot would be complicated.  Figure 2: (A) a smeary dot plot showing...

7. Viral Vectors 101: Gamma-Retroviral Packaging Systems

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   ... be used interchangeably. Are γ-retroviruses replication incompetent? Yes, as with lentiviruses, the genes...

8. Hot Plasmids - May 2022

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   ...clonal multiplexing of cells in live imaging application. Each visual barcode consists of a fluorescent...

9. Antibodies 101: Buffers, Storage, and Conjugates

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   ...compensation.    Working with antibodies can get a bit complicated, but hopefully this helps you successfully select...

10. Antibodies 101: Isotypes

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    ... Isotype controls are used in antibody-based applications including flow cytometry, immunohistochemistry...

11. Multiplex Genome Editing with CRISPR-Cpf1

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    ... a simple and effective method with multiple applications (in vitro, in vivo, transfection, viral transduction...

12. Addgene’s Viral Service - Why Virus? Why Now?

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    ...under a similar model for our Viral Service, we solicited seed money for the new project. New England Biolabs...

13. Clear and Accessible Writing

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    Blog Post

    ...to use in our website and educational content conflicted with plain language guidelines.  Which is why...

14. A Deep Dive into BioBuilder

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    ...whose work is focused on education and education policy. Additional Resources on the Addgene Blog Synthetic...

15. Plasmids 101: Methylation and Restriction Enzymes

    Type

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    ... plays an integral role in regulating genome replication, repairing mismatched basepairs or small indels...

16. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

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    ...are strong and some more moderate; there is an allelic series where the degree to which genes are turned...

17. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type

    Blog Post

    ...relationships can last for an hour or for a year. Some click and some do not. These are all normal outcomes. ...

18. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

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    ...extremely exciting emerging field, with many implications for regenerative medicine, evolution of body...

19. Reproducibility for Everyone: Lessons from an Open Science Collaboration

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    ...could participate. Despite the group not having a public website, all the slides, handouts, and resources...

20. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

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    ...the tumor. How do microenvironment enzymes and metabolic reprograming influence tumors development? Being...

21. Celebrating 15 Years of Scientific Sharing

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    ...law review article we received some entirely unsolicited praise: “Addgene, for one, is a sterling example...

22. Addgene: The First Twenty Years

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    ...for being Addgene. You guys rock." — Andrew York, Calico Life Sciences. Most of all, we’re incredibly thankful...

23. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

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    ...250 nm), and studying endocytosis is further complicated by the reliance on pH-based biosensors used to...

24. RANbodies: Reporter Nanobody Fusions

    Type

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    ...some reporters are better suited for particular applications. Read on to learn more about each reporter or...

25. Summer SciComm Series: Cognitive Load

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    ...learners. If something I was communicating didn’t click, instead of blaming the audience, I would think ...

26. What's New in CRISPR - November 2021

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    ...therapeutically relevant gene targets and expand the application to the possible treatment of genetic disease....

27. Antibodies 101: Polyclonal Antibodies

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    ...are particularly useful for certain research applications. Many secondary antibodies are polyclonal, as...

28. Lighting Up Cell Signaling with Photoswitchable Kinases

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    Blog Post

    ...kinases good for? There are a number of potential applications for photoswitchable kinases.  Below are a few...

29. To Each HIS Own

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    ...proteins within a system. This can be pretty complicated given that a single cell has tens of thousands...

30. Giving gRNAs a Facelift - Synthetic and Beyond

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    ...were developed before CRISPR-Cas9, for use in applications like siRNA and antisense oligonucleotides. Some...

31. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

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    ...which can then be used for downstream cloning applications. While these two strains ultimately perform ...

32. Transferable Skills Guide: Cross-team Communication

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    ...types or purposes of each given container, when applicable Any other relevant information Prescribing an...

33. Addgene's Tips for Plasmid Quality Control

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    ...access and use the materials they need, and avoid duplicating your effort. In addition, if you should choose...

34. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

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    ...CRISPR/Cas systems, however, greatly improved the simplicity of gene activation: rather than requiring protein...

35. How to Prepare for an Industry Interview

    Type

    Blog Post

    ...the same elements as a STAR answer: a setting, a conflict, and a resolution. Stories usually have a theme...

36. Meet an Addgenie: Insights into Grad School, Career Paths, and More

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    ...to your values While going through the co-op application process, my advisor encouraged me to keep my ...

37. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type

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    ...excitation or inhibition upon light (or drug) application.  Genetic manipulation can also reduce expression...

38. Design Tips for Prime Editing

    Type

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    ...its design changes for every new prime editing application. And optimizing its sequence is important, as...

39. Antibodies 101: Stripping and Reprobing Western Blots

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    ... tool to identify a protein of interest in a complicated solution like cell lysate. But they can be a ...

40. Popular Retroviral Vectors and Their Uses in Scientific Research

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    ...Gammaretroviral Vectors: Biology, Technology and Application. Viruses. 2011. PubMed PMID: 21994751. PubMed...

41. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

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    ...glycosylation (CDG) are a group of rare inherited metabolic diseases with severe and life-threatening consequences...

42. Lab Automation at Addgene

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    ...protocol on the Biomek FxP is pretty simple. By clicking a few buttons you can quickly tell the Biomek ...

43. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

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    ...Edition. 2104. Cold Spring Harbor Laboratory Publications, New York. Helma J, Cardoso MC, Muyldermans ...

44. Hot Plasmids and Viral Preps - March 2021

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    ...and should be varied as it can have important implications for the fusion protein effectivity), and a fluorescent...

45. Fluorescent Proteins 101: Introduction to FRET

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    ...nanometers of each other, or measure the tension in the helical structure of spider silk, or the activity of a ...

46. CRISPR in the Clinic

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    ...substantial relief from both disorders. As of publication, submissions to the UK, EU, and USA federal drug...

47. Viral Vectors 101: Calcium Sensors

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    ...Vectors 101: Biosensors Viral Vectors 101: Viral Applications Viral Vectors 101: An Introduction to AAV ...

48. High Complexity Golden Gate Assembly with NEB’s DAD

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    ...Overhang Ligation Fidelity by T4 DNA Ligase and Application to DNA Assembly. ACS Synthetic Biology. https...

49. Adeno Associated Virus (AAV) for Cell and Gene Therapy

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    ...trials support the utility of AAV as a broadly applicable gene delivery tool, aspects of AAV biology should...

50. The Materials Science of Optogenetics Experiments

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    ...Injectable, cellular-scale optoelectronics with applications for wireless optogenetics. Science. 2013;340...