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We narrowed to 354 results for: Plat;

Showing: 301 - 320 of 354 results

  13. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...., 2007; plasmids at Addgene). AAV vectors: A platform for delivering neuronal tracers AAV vectors have...

  14. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...that can be combined with your gRNA and repair templates of choice though modular cloning steps. The Voytas...

  16. CRISPR Guide

    Type
    Guide
    ...genomic edits by copying from a DNA donor template . The donor template contains the desired sequence and is...linear dsDNA templates work better for large edits or insertions. When designing the donor template, incorporating...detection platform. Based on this non-specific cleavage, a Cas13a-based molecular detection platform, termed...pathway. HDR relies on copying DNA from a matching template to accurately repair or fill in the missing sequence...the break ends without the need for a homologous template. Both NHEJ and HDR have their uses, but we’ll ...binding site (PBS) and the desired edits on a RT template. The PBS base pairs to the 3’ flap, and the desired...driving mismatch repair using the edited strand as a template PE4/5 - improved PE2/PE3 to inhibit mismatch repair...

  17. Molecular Biology Reference

    Type
    Guide
    ...most commonly used is the Illumina NGS platform. This is the platform used by Addgene’s sequencing partner...of DNA, or templates, are spatially separated from each other. These attached DNA templates are then amplified... again producing ~1,000 copies of each template. Each template is then replicated using the modified bases...antibiotic(s) to add to your LB media or LB agar plates. Antibiotic Recommended Stock Concentration Recommended...the 4 nucleotides, a DNA polymerase enzyme, the template DNA to be copied, and a primer. A primer is a ...is a high-throughput, multi parallel sequencing platform that can generate sequencing data for up to 600...normal” bases in the reaction. All the sequenced templates are then aligned to each other to assemble the...

  18. Antibody Guide

    Type
    Guide
    ... Enzyme-linked immunosorbent assay (ELISA) is a plate-based assay that uses standard curves to quantify...Overview of application Coat antigens to the well of a plate, either by charge interactions (direct) or by a ...several wash steps. Add primary antibody to the plate. After incubation, wash to remove unbound antibody...Activate the conjugate and read the output via a plate reader. Special considerations The two most common...of interest is attached to the well of a 96-well plate. A conjugated primary antibody is used to bind to...be sorted (separated and deposited into tubes or plate wells) by fluorescence activated cell sorting (FACS...

  19. Adenovirus Guide

    Type
    Guide
    ...also made them the most widely used viral vector platform for vaccine design against a diversity of viruses...AdMax™ is another well used rAdV vector production platform, which like AdEasy™, relies on the homologous ...Tai, P. W. L., & Gao, G. (2021). Viral vector platforms within the gene therapy landscape . Signal Transduction...Curiel, D. T. (2021). Adenoviral vector vaccine platforms in the SARS-CoV-2 pandemic . NPJ Vaccines, 6 (...

  20. Promoters

    Type
    Guide
    ...produced. The antisense strand is referred to as the template strand or non-coding strand as this is the strand...promoter region stably and transcription of the template strand can initiate. The TATA box is a DNA sequence... is the RNA responsible for providing a stable template for the translation of a protein. RNA polymerase...
Showing: 301 - 320 of 354 results