...a journal for formal peer review. Some organizations, including some journals, also support open peer ...committees, as opposed to the opaque (and, admittedly, somewhat meaningless) practice of listing “Submitted” articles...scientific communication needs. Going to present some unpublished results at a conference? Post your working... lack of awareness of the benefits of preprints. Some worry about faulty work being posted as preprints...

...laboratory of Dr. Moshe Szyf at McGill University. Some enzymatic fusions are godsends. Tethering dCas9 ...what was perhaps a slightly smaller population, something else was also suddenly cool: DNA methylation. ...with transcription machinery.   Well, I’ve got some good news: complete DNA demethylation can be achieved...in pluripotent cells at thousands of methylated somatic enhancers. Nat Genet 52, 819–827. https://doi.org... B (1983) Hypomethylation distinguishes genes of some human cancers from their normal counterparts. Nature...

...themselves can sometimes interact in cells and produce background signal (especially for some older split-GFP... example, EBFP2(1-10) can be used with GFP(11)). Some of the articles linked below only provide one fragment...complementary fragment. Next, let's look at a round-up of some new and popular split-FP plasmids from our catalog...interaction is typically irreversible and requires some time for chromophore maturation, splitFAST displays...

...overnight (however this can be done if you already have some leadership experience). I was involved with postdoctoral... tasks as things came up. For example, I updated some sections of the NPA’s website. By volunteering,...style and requirements of the new Board Members. In some instances I felt like I was going back to previous... according to the needs of my committee members. Some members would need much more guidance, direction... action, others would act more independently and some others would need regular re-direction and alignment...

... and set some approximate timelines. Be sure that you keep your commitments, and if for some reason you...while also considering the needs of other people. Some students and postdoctoral fellows are hesitant about... does. You can save yourself and your supervisor some time if you come to every meeting with one or more...need to be more assertive to get your point across. Some supervisors might try to intimidate you with negative...

...donor [7]. Adult stem cells (ASCs) Also known as somatic stem cells, these multipotent stem cells are located...advantages and disadvantages. Here is a table listing some of them: Characteristic ESC ASC Self-renewal...we’ll discuss the two widely used approaches of somatic cell conversion: conversion via iPSC formation,... reprogramming and, direct conversion from one somatic cell type to another, also known as transdifferentiation...pluripotent stem cell lines derived from human somatic cells. Science, 2007. 318(5858): p. 1917-20. PubMed...

... need to be produced in bacteria cells. However, some viruses can be produced in multiple different cell...viral production, partially because they contain some of the necessary machinery to make these viruses...will depend on your specific virus, so this is something you may have to optimize for your specific application...taking your first round of virus, called P1 (or sometimes P0), infecting new Sf9 cells, and getting an amplified... and equipment. Hopefully this post has provided some insight on these differences in viral production...

... (Karki et al., 2021; Kattnig et al., 2016), and some previous attempts at magnetic control of biological... 2016; Duret et al., 2019). But the field has sometimes seen controversy, and the interactions of organic...project, Maria’s screens of AsLOV2 variants showed some significant gains! After a few more rounds of mutagenesis...collected a variety of data that might help formulate some hypotheses. They are excited to see other researchers... develops. And though it took a lot of work (and some luck) to get here, he believes MagLOV is just a ...

...RNA (mRNA). mRNA can then be translated at the ribosome into polypeptide chains that make up the primary...applications In this blog post we will summarize some of the more common expression systems including ...high protein yields if transfection is successful. Some mammalian systems also allow for control over when... up for production of large amounts of protein. Some drawbacks of insect cell expression systems include...transcription and translation machinery. These include ribosomes, RNA polymerase, tRNAs, ribonucleotides and amino...

...For some cells, add a drop of serum to aid in outgrowth. Depending on the cell type, we sometimes add ...colonies are well separated. For this, you need to have some idea of the rate of growth of your specific cell...well-spaced clones. For rapidly growing cells we sometimes use TPP peelaway T-75 flasks to provide a greater...endocrine cells. She can be emailed at ilindberg@som.umaryland.edu.   Additional Resources on the Addgene Blog...

...models for trafficking to the lysosome by fusing Medium-FT to LAMP-2A, a lysosome-associated membrane protein...hypothesized to be a result of chromophore oxidation. Some of the more popular timers (whether as part of protein...cloning and makes your experimental setup less cumbersome. 3. Portability across multiple systems Terskikh...respectively. While Tsuboi et al developed mK-GO somewhat serendipitously while attempting to enhance mKO...

...is the chance of a chromosomal inversion, or just random mutations if the chromosome is repaired with all...small edits that change single base pairs, to chromosomal inversions and large deletions. Many of these...portion can be bypassed by leaving out Cas3 — a tactic some CRISPR transposase systems utilize with type I-F...two or more gRNAs must be used along the same chromosome. During DNA repair, there is a chance that the...

...Populations: I. Cage Studies of Chromosome Replacement by Compound Autosomes in Drosophila melanogaster. ...heterozygotes, the endonuclease cuts the wild-type chromosome and its own gene is used as a repair template.... For example, Drosophila lines with compound autosomes are completely infertile when mated to wild fruit...which would represent proof positive that at least some scientists could not be trusted to handle such a...

...proteins are PA-GFP, Dendra2, and the mEOS proteins. Some proteins are reversibly switchable (e.g. rsEGFP,...biosensors can be found at Addgene. Split FPs – some FPs (e.g. GFP, Venus) can be split into two halves...filters (e.g. GFP also emits yellow light). Note that some combinations of FPs can cause an effect called FRET...brightness is compared to that of EGFP which is set as 1. Some proteins are very dim (e.g. TagRFP657, which has...which is slightly acidic, or synaptic vesicles). Some FPs have different ex/em spectra (e.g. mKeima) or...

...technology and its potential for the treatment of some genetic diseases. I didn’t know it at the time..., including the RPE65 gene responsible for an autosomal recessive, severe, childhood-onset form of blindness...expression in non-dividing cells because they remain in episomal form in the cell’s nucleus. But since nothing...age-related macular degeneration are ongoing and some will hopefully follow in the footsteps of Luxturna...despite promising results in preclinical studies some potential candidates for rare to ultra-rare ocular...

...technology. Can you describe this technology and some of its potential applications? Fujii: We generated...amplification of specific DNA sequences. Thus, we've made some contributions in technology development in biology...different experiences in research labs. What are some similarities and differences you have observed as...publications in a short period of time and may need to do some fashionable projects. However, I hope that they ... they don't stop, they may be able to achieve something that may change the rules of the game. In this...

...but his short arms are causing him some difficulty… To give him some inspiration, we’d love to see how ... upcoming wintertime festivities! Help us spread some cheer by snapping a photo of you and your labmates...will warm up the entire lab (hint: you might need some mini marshmallows…). We’ll choose 4 submissions ...

...genetic similarities with humans. However, rats have some key differences in physiology, intelligence, and...science with early research providing insight into some of the most significant and fundamental biological...in vivo studies. These attributes make frog eggs some of the most useful tools in developmental and biomedical...commonly used species of frog. (Liu et al., 2016). Some of the most popular tools for studying frogs include...world worked to characterize each gene’s function. Some key genetics research with Arabidopsis include understanding...

...While many transgene will fit within this limit, some like prime editing's PE2 enzyme do not. So how do...is the key to fitting large genes into AAV First, some history. In 2008, the Auricchio lab decided to solve...Chamberlain et al., 2016). It’s generally accepted that some form of homologous recombination (HR) occurs via....org/10.1038/mt.2010.205 Trapani I, Colella P, Sommella A, Iodice C, Cesi G, Simone S, Marrocco E, Rossi... Simone S, Colella P, Iodice C, Polishchuk EV, Sommella A, Colecchi L, Rossi S, Simonelli F, Giunti M,...

...provide cells with physical structure, separate chromosomes during mitosis, support transport of organelles...different CIB constructs to the plasma membrane or lysosomes (the earlier article also provided CIB constructs...constructs for targeting Golgi, ER, and endosome membranes) (Figure 4). The authors used SuperPLD to manipulate...A) OptoPLD is recruited to plasma membrane or lysosomes and its activity can be monitored by phosphatidic...enzymes. Conclusion We hope this post has illuminated some of the many tools available for controlling cell...