We narrowed to 869 results for: SOM

...While many transgene will fit within this limit, some like prime editing's PE2 enzyme do not. So how do...is the key to fitting large genes into AAV First, some history. In 2008, the Auricchio lab decided to solve...Chamberlain et al., 2016). It’s generally accepted that some form of homologous recombination (HR) occurs via....org/10.1038/mt.2010.205 Trapani I, Colella P, Sommella A, Iodice C, Cesi G, Simone S, Marrocco E, Rossi... Simone S, Colella P, Iodice C, Polishchuk EV, Sommella A, Colecchi L, Rossi S, Simonelli F, Giunti M,...

...provide cells with physical structure, separate chromosomes during mitosis, support transport of organelles...different CIB constructs to the plasma membrane or lysosomes (the earlier article also provided CIB constructs...constructs for targeting Golgi, ER, and endosome membranes) (Figure 4). The authors used SuperPLD to manipulate...A) OptoPLD is recruited to plasma membrane or lysosomes and its activity can be monitored by phosphatidic...enzymes. Conclusion We hope this post has illuminated some of the many tools available for controlling cell...

... dyes usually spread unidirectionally, there are some reports of bi-directionality. For predominately ...been modified and used as tools for tracing. With some engineering, RABV and HSV, but also others including...vectors have been widely used by neuroscientists for some time due to their advantageous characteristics for...retrograde and anterograde intracellular transport of some AAV serotypes, but overall with rather low efficiency...however, a few reports of trans-synaptic spreading of some AAVs, specifically AAV6 in the retrograde direction...monosynaptic and polysynaptic spreading. AAV vectors cover some of these attributes and their use has allowed for...

...c-MYC is the established model for reprogramming somatic cells. A common method for generating iPSCs to ...-excitable organge fluorescent protein (CyOFP1), some of the exciting properties of mCyRFP1 include a ...with HDR. Coselection efficiency is so high that some applications may permit working with edited “pools...see figure above). 3 Reasons The PSU Ladders Are Awesome Tools: They are comprised of 2 plasmids, pPSU1...looking for the production of ECT-DNA (extra-chromosomal telomeric DNA), which accumulates when T-loops... themselves against bacteriophage infection, but some phages fight back using CRISPR inhibitors. Several...

...biology. From tumor suppressors to growth factors, some of the most clinically-relevant proteins are also...or unique surface epitopes for many proteins, but some proteins do not have such epitopes. These classical...extracellular RNA editors Tracking factors that utilize exosomal transport is quite challenging. Work by Shibata...et al. (2023) used HiBiT to characterize the proteasomal degradation of cystic fibrosis transmembrane ...diminished luminescence signal, associated with endosomal localization of EGFR. This method offered kinetic...

...parvalbumin (PV), somatostatin (SST), vasoactive intestinal peptide (VIP), or lysosomal-associated membrane...Every few months we highlight some of the new plasmids, antibodies, and viral preps in the repository...imaging rapid synaptic events such as in the somatosensory cortex, while iGluSnFR4s produces larger signal-to-noise...(PV) immunostaining (green), in the primary somatosensory cortex (S1) of the mouse brain following retro-orbital...

...We’re making some moves over here at Addgene...and they’re looping? What’s the deal with looping...level up in the lab, and we’ve started collecting some of our favorite (moving) images on a GIPHY page!...so well you need to express yourself. We have something for that too.   This could be you after...emotions (while you’re on a break from expressing some genes of interest).   Blugene is so excited...

... appropriately validated? This post will go over some of the general considerations you should review ...you may need to roll up your sleeves and perform some extra validation experiments yourself.  Goal of ...be concerned that your antibody is binding to something unexpected. This approach has high specificity...sites or alternative splicing that could lead to some form of the target protein to be present and recognizable...can be difficult to find epitope information for some antibodies, which makes it hard to ensure that you...

...students split into two groups for an in-depth look at some of the teams at Addgene. An introduction to plasmids...our Shipping Operations Coordinator: “Kory was awesome!”. The students also got to learn about intellectual... science and business or programming were new to some, who were surprised at “how much business there ...researchers, so Outreach Scientist Jessica Welch showed some highlights of his travels from 2015, including a...

...career paths. Check out Joanne's Reddit AMA Here are some concrete things you can do to develop those transferable...timelines Be a mentor in a formal program Start something (a journal, a science lunch club, a biotech club...division of labor Serve on a committee and do something big like plan a conference Recruit many supporters...departments want to be able to tell that you know about something and are passionate about it.   Maintain a content-useful...

... their work. Thousands of people showed up; some with some really great signs. The Congressman for Albany...Harvard Medical School working on human artificial chromosomes. Montreal, QC, Canada 45.5017°N 73.5673°W – Vanessa...lab where the groundbreaking research happens, sometimes forgetting how to engage with friends, family,...science professionals and supporters. I'm raising some little scientists (as you can see in the picture...

...Flow cytometers however are highly versatile and some can accommodate up to 30 different colors. This ...the tag can alter the structure of the protein. In some cases this can lead to changes or a complete loss... lipids including those of the cell membrane. In some cases, this can mask epitopes such that immune complexes... complexes will no longer recognize them. Sometimes, users will combine methods and fix with formaldehyde...overview of flow cytometry for single cell analysis and some useful background information for setting up antibody...

...this blog is here to help. Here, we will outline some fundamental characteristics to consider when selecting...make your selection easier, as well as highlight some recent advancements within this field. Fundamental...be covalently attached to self-labeling proteins. Some common examples of these self-labeling proteins ... and its propensity to bind cellular components. Some dyes target specific organelles like mitochondria...technically cell-permeable, its use in live cells can sometimes result in cellular toxicity due to the permeation...

...project. You’ve met someone whose career interests you or you want to pursue someone doing a job you wish...potential to alienate connections. If meeting with someone to ask about a job or a particular position at ...interest in a job. Ask openly to be connected to someone who can help you land that position such as a direct...

...expertise with lots of free beer.” He also mentions that some of the team will be moving to flats with gardens... in five years and hopefully I'll have worked something out!” We will get back to you on that, Tom! Connie...”. He also cooks and cleans with his family, “sometimes listening to audiobooks.” Church and his family... family. And as the school year winds down, take some time to have fun outside the lab! How does your ...

...parameters of CRISPR experimental design. Let’s dive into some structural biology! Major features First, we’ll ...is recognizing the PAM on DNA, the CTD is also sometimes called the PAM-interacting (PI) domain. The guide...al., 2013). As mentioned above, however, Cas9 sometimes tolerates mismatches in the next 8-10 bases after...7S4X), shown opaque (left) and transparent (right). Some nucleotides of the non-target DNA strand are not.../doi.org/10.1016/j.cell.2014.02.001 Osuka, S., Isomura, K., Kajimoto, S., Komori, T., Nishimasu, H., Shima...

... in scicomm has grown and now it feels like an awesome bionerd hobby but also a viable away-from-the-bench...was leaving a writing position and looking for someone who might take over. I like writing and science...communicator than a science writer these days. I do some public speaking and a bit of video work for webinars...after joining IDT when his boss was looking for someone to take over hosting them. Since then, webinars...

...drug downstream. In some cases, there may already exist a drug which targets some of the candidate genes...onset. These hits can reveal individual genes or sometimes even genetic pathways that are worth investigating...drug development. Due to the difficulty of drugging some targets – the dreaded ‘undruggable’ proteins – having...

...every two weeks or every month is long enough that some experiments can get done and data analyzed between...scientists edit the same document may sound like something out of your nightmares, shared ownership of materials...the output that will come from the collaboration. Some things to figure out are timelines for experiments...communication within an existing collaboration, or start something new! Additional resources on the Addgene blog...

...thought we’d take some time to introduce you to the idea of the Freshers Fair and some of the great biological...scientific networks in their many years of study to come. Some Freshers Fairs can include fairground rides and ...know of a society or event which would benefit from some Addgene support? Please get in touch, as we aim ...