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Showing: 301 - 320 of 961 results

  1. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    ...restriction digestion to anneal to one another and form a complete, circular plasmid. You then add DNA ligase...You should perform, at minimum, two transformations after a ligation: 1. Control Transformation containing...contamination from uncut plasmid. 4. Transformation Transform your ligation reaction into your bacterial...Read on for an in-depth breakdown of how to do perform restriction digests.   Overview of the restriction...Bromide Pre and Post UV Light 0.5 For more information on these stains see the Bitesize Bio Blog and... how many colonies you should expect in the transformation due to background re-circularization and contamination...competent cells. For most standard cloning, you can transform 1-2μl of your ligation reaction into competent...

  3. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...Addgene's Viral Plasmids and Resources page for more information. Plasmids Expressing Spike and Spike Variants...

  4. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...-induced cytidine deaminase (AID). Want more information on the wide variety of Cas enzymes? CasPEDIA ...

  6. AAV Molecular Tools

    Type
    Collection
    ...fill out our form to let us know what you would like to see available in viral prep format. Please note...

  7. Synthetic Biology - Overview

    Type
    Collection
    ...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...world to facilitate the sharing of parts and information. Addgene can help you to share your synthetic...Jaramillo Lab Riboregulation Keasling Lab BglBrick Platform , Terpenoid Production , and Propionate Induction...

  8. Serotype Testing AAV

    Type
    Collection
    ...fill out our form to let us know what you would like to see available in viral prep format. Please note...economical choice that can be used to validate the performance of various serotypes before conducting larger...larger scale experiments. For more information, see our full AAV inventory or our AAV production page . Note...direct GFP expression from the CAG promoter. For information about each catalog item, including the titer ...expression from the human synpasin promoter. For information about each catalog item, including the titer ...

  9. COVID-19 Resources

    Type
    Collection
    ...COVID-19 research? Fill out this Suggest a Plasmid form. If the plasmid has been described previously we...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...inexpensive platforms to reliably detect nucleic acids at the atomolar level. For more information read Addgene's...denoted in the Industry column below. For more information on ordering from a for-profit entity, please ... ID Plasmid Description Industry PI For more information on these genes, see the links and references ...SHERLOCKv2). The Broad Institute has published information and protocols for utilizing SHERLOCK to detect...(DETECTR). Mammoth Biosciences has published information and protocols for utilizing DETECTR to detect...

  10. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ... molecules that cannot function in isolation but form a fully functional Cas9 upon dimerization. Splitting...inactive dCas9 and VP64, respectively. CibN and Cry2 form a heterodimer in response to blue light, which will...this system if a small contaminating PCR product formed from primer dimers and containing the missing codons...limitation to this strategy is that each "bit" of information must be controlled independently; that is, the...the signal to record one bit of information must not act on any of the other bits in order to achieve ...capable of recording 11 bits (1.375 bytes) of information. This leads to 2048 (211) possible combinations...designed to minimize the possibility of dimer formation. Prosser et al., Biotechnol Lett. 2015. 37:383...

  11. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    ... criticism? Do they encourage diversity in all forms? How are their organizational skills? Are they... most informative way of gathering data. Former lab members can give open, revealing information about...attention and interaction (and I did). Some of the information you will need is about you. It’s worth taking...discussing career opportunities with the lab head. Former lab members can give you a fuller picture. Consider...physical or social isolation, withholding necessary information Overwork – including undue pressure, impossible... Or is it teamwork that brings out your best performance and learning? Everyone’s different so give some... on relevant papers? Will you have access to information from other labs with skills that you can learn...

  12. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    ...feel you rolling your eyes, but even if this ideal form of pre-registration doesn’t come to fruition any... it easier to find information about research tools. BenchSci collates information about antibodies while...can find a variety of additional resources and information about organizations promoting reproducibility...S. Flier, Researcher at Harvard Medical School, former dean of the faculty of Medicine at Harvard University..., generating new therapeutics, and sometimes formulating new biological concepts. However, rates of reproducibility...reproducibility. Cause 1: Inability to repeat previously performed experiments Researchers should be able to follow... tools that make it easier to share in-depth information about reagents, protocols, analysis tools, and...

  13. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ... available from Rudolf Jaenisch’s lab in plasmid form or as ready-to-use lentivirus. Figure 2: ... available from Rudolf Jaenisch’s lab, with the former also available as ready-to-use lentivirus. ...ME, et al (2018) Creation of versatile cloning platforms for transgene expression and dCas9-based epigenome...

  14. Antibodies 101: Validation

    Type
    Blog Post
    ... or alternative splicing that could lead to some form of the target protein to be present and recognizable...your antibody performs as expected in one application does not mean that it will perform as expected in...so you use your bioinformatics skills (or your friendly neighborhood bioinformatician) to look at RNA ...doesn’t necessarily mean that you yourself have to perform extensive validation experiments for every antibody...data, you may need to roll up your sleeves and perform some extra validation experiments yourself.  Goal...researchers can use to show that their antibody performs as expected. One of the benefits of these pillars...levels tend to correspond to protein levels, so you perform immunohistochemistry with your antibody on these...

  15. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...crosslinking or tilted-stage imaging. A truncated form of a tardigrade LEA, RvLEAMshort, protected samples...PASSIGE efficiency (Pandey et al., 2024). The best-performing variant yielded an average integration efficiency...

  16. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    ...individual bacterial cells to form colonies on a plate and then we performed colony PCR on the register ...Biological research has produced a massive amount of information regarding which regulatory proteins, signaling...homogenous for one state, regular PCR may not be very informative – each primer pair will amplify even if its target..., each Sanger sequencing reaction can only be performed on one register at a time. If a particular application...considered as an alternative. The qPCR method can be performed on a sample of multiple registers. However, it...recognition site array on the register. RSM (See performance data from the indicated figures in our paper ...have observed that certain GRSMs may still not perform as expected, perhaps due to idiosyncrasies that...

  18. Antibody Guide

    Type
    Collection
    ...light chains. These four domains come together to form a “Y” shape, as shown in Figure 1. The two arms ...the five human isotypes. Some isotypes naturally form multi-antibody units, while others exist as monomers...may want to identify or collect the protein in any form it may be present in. However, polyclonals can vary...antibody, for each antibody used. Blank controls in the form of sample buffer must also be run. Unique samples...fluorophores. Horseradish peroxidase (HRP) HRP performs a chemiluminescent reaction in the presence of...activated and its output measured. This method can be performed using the indirect detection method via a conjugated... able to recognize the protein in its native conformation. Attachment of the antibody to the beads is ...

  19. Plan Your Experiment

    Type
    Collection
    ...potential consequences of having a non-functional form of your protein expressed in your system. HDR : ... to produce the Cas enzyme, and the enzyme then forms a complex with the gRNA for CRISPR activity. Because...purified Cas protein and in vitro transcribed gRNA to form a Cas-gRNA complex. You then delivery the RNP to... and large insertions or deletions. For more information on the wide range of CRISPR technologies, visit...Selecting the genetic manipulation you plan to perform will help you narrow down which components you’...edit, ideally less than 10 bp away. For more information, read our three tips to improve HDR efficiency...your Cas protein and gRNA, which you generally perform by chemical transfection or electroporation. Expression...

  20. Recombinases AAV Preps

    Type
    Collection
    ...fill out our form to let us know what you would like to see available in viral prep format. Please note...expression. See our Cre-lox Plasmids page for more information about these molecular tools. Cre AAV ID Name ...
Showing: 301 - 320 of 961 results