We narrowed to 482 results for: vent

1. FlipGFP, a novel fluorescence protease reporter to study apoptosis

   Type

   Blog Post

   ...activation. Normally, β10-11 are antiparallel. To prevent binding, the β strands in FlipGFP were redesigned...

2. Reagent Repositories Are Speeding up Science During the Pandemic

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   Blog Post

   ...science research, including over 4,000 cell lines. Seventeen of these cell lines were used to investigate the...

3. Learning Technical Skills at a Lab Central Workshop

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   Blog Post

   ...includes a step that both fragments the DNA and prevents it from forming concatemers later. The "FS" stands...

4. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

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   Blog Post

   ...People are already discussing at tables and the event hasn't even started yet! @neurosymp will be interesting...

5. Plasmids 101: Gram Negative and Positive Bacteria

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   Blog Post

   ...Gram began using it as a classification system. Eventually, any bacteria that retained the stain became ...

6. Four Factors that Differentiate the Stem Cell Field

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   Blog Post

   ...regarding topics such as informed consent (How do we prevent stem cell lines from becoming the new HeLa cells...

7. Management for Scientists: Delegating is Key

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   Blog Post

   ...approachable mistakes will be made that could have been prevented.  Make sure everyone understands that no question...

8. Advice for Scientists Starting a Lab

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   Blog Post

   ...postdoc come to an end and your next professional adventure begins. While many career pathways exist for scientists...

9. From Lab Bench to Leadership: Transitioning into Management as a PhD

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   Blog Post

   ...industry, but the transition wasn’t easy - I had to reinvent myself and redefine the value I could bring to...

10. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

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    Blog Post

    ...or polymerization will occur at that position, preventing T4 from chewing back the entire 10–12 bases. ...

11. Flow Cytometry Readouts: Yes, No, and Everything in Between

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    Blog Post

    ... Addgene blog Antibodies 101: Flow Cytometry Conventional vs Spectral Flow Cytometry Beyond Surface Labeling...

12. Antibodies 101: Flow Cytometry Controls

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    Blog Post

    ...Antibodies 101: Flow Cytometry Antibodies 101: Conventional vs. Spectral Flow Cytometry Resources on Addgene.org...

13. Viral Vectors 101: Inverted Terminal Repeats

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    Blog Post

    ... headache. How do I maintain ITR integrity and prevent unwanted mutations?  During propagation, mutants...

14. Viral Vectors 101: Biosensors

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    Blog Post

    ...give a readout for whatever the bio-recognition event is (fluorescence, pH, etc.). The transducer will...

15. A Deep Dive into BioBuilder

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    Blog Post

    ... for teachers, to schedule a three-day training event for a local school’s Science Department, or to simply...

16. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

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    Blog Post

    ...experiments. But, there have been two huge challenges preventing us from testing this hypothesis: There was no...

17. Antibodies 101: Buffers, Storage, and Conjugates

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    Blog Post

    ...dark, opaque vial or wrapped in aluminum foil to prevent ambient light from degrading them. You’ll also ...

18. Antibodies 101: Monoclonal Antibodies

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    Blog Post

    ... by sequencing the heavy and light chains. To circumvent any possible genetic drift, scientists can freeze...

19. 7 Tips to Secure a STEAM Internship This Summer

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    Blog Post

    ...certain that everyone you live with is aware of the event to limit all distraction. On the day of the interview...

20. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

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    Blog Post

    ... time to choose your own protein purification adventure. You want to purify your favorite protein (YFP...