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We narrowed to 369 results for: Cre expression vector

Showing: 321 - 340 of 369 results

  1. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...cloning method. When using CRISPR, you will need to express both a Cas protein and a target-specific gRNA in...both the gRNA and Cas protein act as all-in-one vectors, but their function is often limited to a single...On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety...Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes...

  2. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...break (DSBs) at a specific location based on a co-expressed gRNA-defined target sequence. DSBs are preferentially...cloning method. When using CRISPR, you will need to express both a Cas protein and a target-specific gRNA in...both the gRNA and Cas protein act as all-in-one vectors, but their function is often limited to a single...On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety...Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes...

  3. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...j.bbrc.2013.08.013. Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...using does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...repressor peptide like KRAB, can knock down gene expression by interfering with transcription. Design your...cloning method. When using CRISPR, you will need to express both a Cas protein and a target-specific gRNA in...both the gRNA and Cas protein act as all-in-one vectors, but their function is often limited to a single...

  4. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ... 101137 pSNAP-tag (T7) Vector SNAP-tag Empty backbone for bacterial expression under T7 control 101135...pSNAP-tag (m) Vector SNAP-tag Empty backbone for stable and transient mammalian expression 101133 pSNAP-CaaX...pCLIP-tag (m) Vector CLIP-tag Empty backbone for stable and transient mammalian expression 101134 pCLIPf-H2B...101127 pMCP-tag (m) Vector ACP/MCP-tag Empty backbone for transient mammalian expression 101126 pACP-tag ...pACP-tag (m)- 2 Vector ACP/MCP-tag Empty backbone for stable and transient mammalian expression 101132 pMCP-GPI...Synthases (AcpS and Sfp, respectively) may be expressed and purified as described previously in Stachelhaus...-surface proteins, and are useful for labeling secreted proteins with disulfide bridges such as antibodies...

  5. Promega Plasmid Collection

    Type
    Collection
    ...luciferase reporter vectors for sensitive gene expression analysis and fusion vectors containing tags such...luciferase reporter vectors for sensitive gene expression analysis and protein fusion vectors for detecting ... from an enzymatic reaction to measure subtle expression changes with high sensitivity. These assays are...enables highly sensitive detection at endogenous expression levels. NanoLuc fusions are useful for both intracellular...want minimal disruption of endogenous protein expression and interactions, such as exploring viral biology...HaloTag technology is optimized for various protein expression systems including E. coli , mammalian cells, ...interest is fused to either LgBiT or SmBiT and expressed in live cells. When the two proteins interact,...

  6. CRISPR Plasmids - Plants

    Type
    Collection
    ...Marker PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...using does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...CRISPR expression plasmids that have been designed for use in plants. CRISPR...repressor peptide like KRAB, can knock down gene expression by interfering with transcription. Design your...cloning method. When using CRISPR, you will need to express both a Cas protein and a target-specific gRNA in...

  7. Open Enzyme Collection

    Type
    Collection
    ...enzymes into an expression vector of their choice and then transform into E. coli for expression and purification...Other Enzymes You may also like… Bacterial Expression Vectors Synthetic Biology Plasmids BIOFAB Plasmid...their own enzymes by cloning them into an expression vector of their choice and transforming into E. coli... and protocols for expression in the Open Bioeconomy Lab's crowd-sourced expression guides (Link opens...and codon-optimized the Open Enzymes parts for expression in Escherichia coli bacteria. Researchers can...purification ( pET28a-LIC or other pET vectors are recommended). The selection marker for all plasmids in ... are also compatible with the E. coli Protein Expression Toolkit (Link opens in a new window) — all Open...

  8. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...protein. Plasmid Color Expression Description pPROEX Aqua Cyan Bacterial Expresses Aquamarine with N-terminal...Cyan Mammalian Expresses mammalian optimized Aquamarine mCerulean N1 Cyan Mammalian Express a gene of interest...Cyan Mammalian Expresses mammalian optimized CyPet pCyPet-His Cyan Bacterial Expresses CyPet with C-terminal...Mammalian Express a gene of interest fused to the C-terminus of SCFP3A Amber N1 Yellow Mammalian Express a gene...Mammalian Express a gene of interest fused to the C-terminus of Amber mVenus N1 Yellow Mammalian Express a gene...Yellow Mammalian Expresses mammalian optimized YPet pYPet-His Yellow Bacterial Expresses YPet with C-terminal...Mammalian Express a gene of interest fused to the C-terminus of SYFP2 Clover Green Mammalian Expresses Clover...

  9. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    .... PubMed Central PMCID: PMC4523203. Many empty vectors containing these fluorescent proteins are available...available on our FRET Resource page and can be used to create fusions with a gene of interest. These plasmids...biosensors too, as another laboratory may have already created the sensor that you need. FRET biosensors designed... endogenous state that you want to measure (overexpressing a gene or protein after transfection). Future...multiple FRET biosensors simultaneously would further increase the amount of correlated information available...

  10. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...Publication Empty Prime Editing gRNA Vectors A selection of empty gRNA vectors suitable for prime editing are...below. Use the search bar to find a gRNA vector based on expression system, promoter, the type of gRNA (e.g...Cas9. ID Plasmid Expression System Promoter Guide RNA Type Cloning Enzyme Co-expressed Cas9 Selection PI...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian... editing plasmids. Plasmids are available for expression in mammalian systems, bacteria, plants, and Drosophila...another round. Figure 1: Overview of prime editing. Created with BioRender.com. The original prime editing ...

  11. CRISPR Plasmids - Activate Gene Expression

    Type
    Collection
    ...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian... plasmids for activating or upregulating gene expression in various model organisms. CRISPR...transcriptional activation. Plasmids are available for expression in mammalian systems, bacteria, Drosophila, plants...fused to a transcriptional activator peptide can increase transcription of a specific gene. Design your ...activity, experimental considerations, and more, created and maintained by the Doudna Lab . Browse, sort...

  12. CRISPR Plasmids - Repress Gene Expression

    Type
    Collection
    ...using does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...repressor peptide like KRAB, can knock down gene expression by interfering with transcription. Design your...transcriptional inhibition. Plasmids are available for expression in mammalian systems, bacteria, plants, and yeast...activity, experimental considerations, and more, created and maintained by the Doudna Lab . Browse, sort...

  13. CRISPR Plasmids and Resources

    Type
    Collection
    ...visualize genomic loci. gRNAs Empty gRNA Vectors Select an empty vector for your experiment based on a variety... fused to an activator peptide activates gene expression. Interfere dCas9 alone, or fused to a repressor...epigenetic modifier alters chromatin structure/gene expression. RNA Targeting RNA Targeting Type VI CRISPR systems...Other Applications Screen CRISPR libraries are a powerful tool for genome-wide screening. Purify dCas9 fused...

  14. Chemogenetics Plasmids

    Type
    Collection
    ...Service: Chemogenetics AAV Blog: Chemogenetics Viral Vectors 101 Blog: Chemogenetics vs. Optogenetics Chemogenetics...a scientist to control signaling within cells expressing these engineered receptors. Chemogenetic receptors... the specific cell type where the receptor is expressed, these receptors can be used to artificially activate...chemogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter Tags Eligible for Viral Packaging...PSEMs). PSAMs are coupled to an ion pore domain to create a ligand-gated ion channel. Whether a PSAM is activating...

  15. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...the 20-mer is not G. sgRNA expression from the U6 promoter of the pX330 vector is enhanced by the inclusion...including additional nucleotides for cloning and expression purposes. NOTE: Here we discuss the usage of ...42230). This plasmid allows for the simultaneous expression of sgRNA and SpCas9, but does not contain markers...or constructs in which multiple sgRNAs may be expressed from a single plasmid. Add “CACC” before the 20...s reverse complement for cloning into the pX330 vector using BbsI restriction enzyme ( Table 3 ). Add ...Prepare a 50 μl reaction mix: 100 ng circular pX330 vector, 1.0 μl annealed oligos (1 μM; see step 3.1.4 )...Cas9 construct (10 μg total). Add 0.5 μg of GFP expression construct. Electroporate cells with 250 volts...

  16. Distribution to Industry

    Type
    Collection
    ...while also adding additional filters such as expression, vector type, species, and more. Plasmids Browse ...for Golden Gate assembly. MoClo Toolkit Plant Expression Sylvestre Marillonnet 95 plasmids for cloning...Libraries CRISPR Thorsten Stiewe Use these donor vector libraries for CRISPR/Cas9-mediated saturation genome...Phagemid Synuclein VHH Immune Library Screening Messer and MJFF Created for research in Parkinson's Disease.... How to Order Creating an Account: Before placing your order, you will need to create an account and ...cloning and assembling standardized parts to create eukaryotic multigene constructs. ONE-GO Biosensors Kit ... our website. Placing an Order: After you have created an account, you can place orders through our website...

  17. Cancer Research Plasmids and Resources

    Type
    Collection
    ...to permit construction of RAS pathway gene expression vectors for in vitro and in vivo research. TP53 Mutagenesis...Broad Target Accelerator Plasmids: Collection of vectors from the Boehm , Hahn , Meyerson , and Root Labs...pan-cancer” analyses. Cancer Pathways ORFs Kit : Set of vectors from the Sabatini & Wood Labs containing cDNAs ... Lab RAS Pathway Clone Collection 2.0 : Set of vectors from the Esposito Lab for use with the Gateway®...signaling studies, including mutant, knockdown, and overexpression constructs. Each collection page listed below... Tackling Cancers’ Drug Resistance with a New Screening Kit (David Sabatini & Kris Wood) National Cancer...

  18. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...getting all components of the Cascade complex to express equally. To combat this, some CRISPR-Cas3 plasmids...Cascade-Cas3 plasmids. Plasmids are available for expression in mammalian systems, bacteria, and plants. Mammalian...to deletions generated by Cas9, large deletions created using Cas3 have fewer off-target alterations and... Figure 1: Overview of Cascade-Cas3 mechanism. Created with BioRender.com. Browse, sort, or search the...

  19. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...gRNA expression vectors available from Addgene....Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...experimental use of these plasmids. ID Plasmid Gene/Insert Vector Type PI Publication Do you have suggestions for...activity. The variety in sequence decreases the gRNA activity and increases off-target effect. Which species...requirements, experimental considerations, and more, created and maintained by the Doudna Lab . gRNA Plasmids...

  20. Feng Zhang Multiplexed Overexpression of Regulatory Factors (MORF) Collection

    Type
    Collection
    ...genes encoded by 3,548 isoforms and two control vectors. The individual clones, or the pooled library containing...factor isoform in pooled screens. This collection and library can be used for screening transcription factors...available at Addgene. The collection can be used for screening transcription factors for cellular programming...Depositor Collections MORF Feng Zhang - Multiplexed Overexpression of Regulatory Factors (MORF) Collection You...window) . TF ORF barcode PCR for NGS QC and bulk screening (PDF, 85 KB) TF ORF barcode dial out PCR for 10X...bp sequence, to simplify identification during screening. Library ID PI Description MORF library 137000...
Showing: 321 - 340 of 369 results