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We narrowed to 365 results for: Fus

Showing: 321 - 340 of 365 results

  1. Synthetic Biology - Assembly Standards Guide

    Type
    Collection
    ...TACTAGAG or TACTAG Features: 8 bp Scar; No In-Frame Fusions; Coding Prefix alleviates RBS-CDS issues. Prohibited...6 bp scar encodes Ala-Ser; Allows for in-frame fusions; NheI is rare in E. Coli genome. Prohibited Restriction... 10 w/ start and stop codons; Creates in-frame fusions w/ linker Thr-Gly; Use N-parts prefix to preserve...

  2. CRISPR Plasmids - Yeast

    Type
    Collection
    ... Publication Base Edit Catalytically dead dCas9 fused to a cytidine deaminase protein becomes a specific...PI Publication Activate Catalytically dead dCas9 fused to a transcriptional activator peptide can increase...Publication Interfere Catalytically dead dCas9, or dCas9 fused to a transcriptional repressor peptide like KRAB...

  4. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...library is fused to the N-terminal portion of split mCherry, while a phosphobinding domain is fused to the...expressing and purifying the phosphosite library as GST-fusion peptides (“mode #1” expression). The mode #1 phosphosite...

  5. Lentivirus Plasmids

    Type
    Collection
    ...variants. Jacks 19319 pLJM1-EGFP 3rd CMV-driven EGFP fusion; can be used for cDNA expression; puro resistance... of pULTRA. Moore 19319 pLJM1-EGFP 3rd for EGFP fusion; PGK driven puromycin Sabatini 25895 pLX301 3rd...pLV-mCherry 3rd CMV driven expression of mCherry fusion. See plasmids 36083 and 36086 for other FP variants...

  6. COVID-19 Resources

    Type
    Collection
    ...Coronavirus Class I Fusion Protein Upstream of Rather than Adjacent to the Fusion Peptide . TACE - Heurich...SARS-CoV Spike protein, activating its membrane fusion function.( 5 ) TACE - (ADAM17) a metalloprotease...

  7. Zhang Lab CRISPR Page

    Type
    Collection
    ... RNA (sgRNA), which is derived from a synthetic fusion of the CRISPR RNA (crRNA) and the trans-activating...components: A nucleolytically inactive Cas9-VP64 fusion A sgRNA incorporating two MS2 RNA aptamers at the...This is a AAV plasmid for sgRNA cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei. Return...

  8. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ...CRISPR plasmids with dCas9 fused to FokI for creating double-strand breaks (DSBs).... Posts A catalytically inactive Cas9 (dCas9) is fused to FokI nuclease. When FokI dimerizes, it generates...

  9. Michael Davidson Fluorescent Protein Collection

    Type
    Collection
    ...Davidson lab fluorescent protein fusion constructs and backbones....with different fluorescent tags for you to create fusion proteins with your gene of interest. Please visit...

  10. CRISPR Plasmids for Genomic Visualization

    Type
    Collection
    ...Posts Using catalytically inactive Cas9 (dCas9) fused to a fluorescent marker like GFP, researchers have...microscopy in living cells. Alternatively, gRNAs can be fused to protein-interacting RNA aptamers, which recruit...

  11. CRISPR Plasmids - Purify Genomic Loci

    Type
    Collection
    ...DNA bound by the gRNA. An epitope tag(s) can be fused to dCas9 or gRNA for efficient purification. Various...antibody. Biotin tagging of dCas9 can be achieved by fusing a biotin acceptor site to dCas9 and co-expressing...

  12. Recombinases AAV Preps

    Type
    Collection
    ... pAAV-Ef1a-mCherry-IRES-Cre EF1a mCherry (not a fusion tag) 8, rg* Deisseroth 55636 pAAV-EF1a-Cre EF1a...pAAV-EF1a-mCherry-IRES-Flpo EF1a mCherry (not a fusion tag) 1, rg* Deisseroth 55637 pAAV-EF1a-Flpo EF1a...

  13. Retrovirus Plasmids

    Type
    Collection
    ...Tetracycline inducible expression of GFP T2A Cre fusion in mammalian cells Foijer 47916 pRXTN CMV/MSV pRXTN...rapid loss of dsRed and the activation of your gene fused to eGFP expression Clevers 18760 MSCV IRES Luciferase...

  14. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...Promoter PI Publication Prime Edit Cas9 H840A nickase fused to a reverse transcriptase (RT) is capable of installing...PI Publication Activate Catalytically dead dCas9 fused to a transcriptional activator peptide can increase...

  15. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...transcriptase (RT) is fused to the C terminus of Cas9 H840A nickase. The fusion enzyme is capable of installing...

  16. Microbiology Resources

    Type
    Collection
    ...Lab MultiSite Gateway Kit : Creating three-part fusion proteins - Planas and Santalucia Labs pCri System...Gateway kit : 285 vectors for expression, markers, fusion proteins, etc - Lindquist Lab Synthetic Biology...

  17. Zebrafish Plasmid Collection

    Type
    Collection
    ...neurodegenerative disorders. Fluorescent Protein Fusion Construct Set - Amro Hamdoun Lab. A useful “starter...starter kit” for screening fluorescent protein fusion expression in organisms or cells in which an exogenous...

  18. Lentiviral Vector Guide

    Type
    Guide
    ...the transfer plasmid contains a chimeric 5’ LTR fused to a heterologous promoter (often CMV or RSV), eliminating...Hybrid promoter; 5' LTR is partially deleted and fused to a heterologous enhancer/promoter such as CMV ...DNA integration and its effect on clinical pre-infusion CAR T-cell products. Journal of Translational ...

  19. Zhang Lab's CRISPR Frequently Asked Questions

    Type
    Collection
    ...never used the Ex Taq. In our hands, Herculase II Fusion polymerase or Kapa Hifi Polymerase work very well... (meaning the mature processed form of crRNA is fused chimerically to a mature tracrRNA), the spacer used...

  20. Retrograde AAV viral preps

    Type
    Collection
    ...pAAV-CKIIa-stGtACR2-FusionRed CamKII Inhibitor Optogenetics Yizhar 105677 pAAV_hSyn1-SIO-stGtACR2-FusionRed Syn Inhibitor...
Showing: 321 - 340 of 365 results