We narrowed to 344 results for: GAI

...signal in these assays (Dempster et al., 2019). Again, the experimental strategy is clear: for any gene...

...DNA ligase, are naturally able to discriminate against ligating substrates containing base pair mismatches...

...and Red (ReNL; plasmid #85203) NLuc fusions. The Nagai lab has additionally deposited more than 25 fusions...

...multiplicity of infection (MOI=ratio of virus to cells). Again, my tip here is to test several protocols as the...

...temperature allows the repressor to re-nature and again repress expression of exo, beta and gam.  A major...

...principles of this classification system will help you gain a deeper appreciation and understanding of CRISPR...

...complexes - from the cell-lysate using antibodies against the target protein. The captured protein complexes...

...lymphocytes from the bone marrow to the thymus where they gain their specificity or from the thymus to the lymph...

...briefly go through each section of this article again. In the beginning, we talked about the relationship...

...in any NIH grant application! Using FRET, you may gain a new structural understanding of your protein that...

...for sufficient opsin to reach the axon terminal. Again, this must be empirically determined in your own...

...precise location. The end of the heteroduplex rests against the RuvC nuclease domain, which stabilizes the ...

...pick one or the other. Which prime editor is which again? We’re glad you asked. Hopefully this table helps...

...succeed. With respect to de novo sgRNA design, I again separate it into two categories. On the one hand...

...run and the difference in CT values is plotted against the log input concentration for each dilution. ...

...rendering the Cas9 non-functional when split but regaining functionality when the halves are reassociated...

...used to protect the RNAs from cellular defenses against ssRNA: phosphatase treatment, substituting cytidine...

...itself, will protect the HR template from the Cas9. Again, if it's within coding region make sure it's a silent...

...and stem cells Chimeric envelope genes have also gained popularity to further enhance both infectivity ...

...utilize CRISPR Cas9 systems to defend themselves against bacteriophage infection, but some phages fight ...