We narrowed to 344 results for: GAI

1. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

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   Blog Post

   ...modifications, and other factors that AAV utilizes to gain entry into the cell (Dudek, AM. 2018).  If the serotype...

2. Plasmids 101: Using Transposons in the Lab

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   Blog Post

   ...mutagenesis screens that detect loss-of-function or gain-of-function mutations. In these screens, the transposons...

3. When Fidelity Matters: A frank discussion about ligase fidelity

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   Blog Post

   ...DNA ligase, are naturally able to discriminate against ligating substrates containing base pair mismatches...

4. Quick Guide to All Things Lentivirus

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   Blog Post

   ...multiplicity of infection (MOI=ratio of virus to cells). Again, my tip here is to test several protocols as the...

5. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

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   Blog Post

   ...temperature allows the repressor to re-nature and again repress expression of exo, beta and gam.  A major...

6. Typing CRISPR Systems

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   ...principles of this classification system will help you gain a deeper appreciation and understanding of CRISPR...

7. Antibodies 101: Designing Your First Flow Panel

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   Blog Post

   ...briefly go through each section of this article again. In the beginning, we talked about the relationship...

8. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

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   Blog Post

   ...complexes - from the cell-lysate using antibodies against the target protein. The captured protein complexes...

9. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

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   Blog Post

   ...lymphocytes from the bone marrow to the thymus where they gain their specificity or from the thymus to the lymph...

10. Special Delivery: Fluorophore Targeting for FRET Studies

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    Blog Post

    ...in any NIH grant application! Using FRET, you may gain a new structural understanding of your protein that...

11. A Primer on Optogenetics: Introduction and Opsin Delivery

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    Blog Post

    ...for sufficient opsin to reach the axon terminal. Again, this must be empirically determined in your own...

12. A Needle in a Base-Stack: Cas9 Structural Biology

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    Blog Post

    ...precise location. The end of the heteroduplex rests against the RuvC nuclease domain, which stabilizes the ...

13. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

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    Blog Post

    ...pick one or the other. Which prime editor is which again? We’re glad you asked. Hopefully this table helps...

14. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

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    Blog Post

    ...succeed. With respect to de novo sgRNA design, I again separate it into two categories. On the one hand...

15. Lentiviral Vector Guide

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    Guide

    ...and stem cells Chimeric envelope genes have also gained popularity to further enhance both infectivity ...

16. Optogenetics + CRISPR, Using Light to Control Genome Editing

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    Blog Post

    ...rendering the Cas9 non-functional when split but regaining functionality when the halves are reassociated...

17. Delivery Methods for Generating iPSCs

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    Blog Post

    ...used to protect the RNAs from cellular defenses against ssRNA: phosphatase treatment, substituting cytidine...

18. Deep Dive: qPCR

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    Blog Post

    ...run and the difference in CT values is plotted against the log input concentration for each dilution. ...

19. CRISPR/Cas9 FAQs Answered!

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    Blog Post

    ...itself, will protect the HR template from the Cas9. Again, if it's within coding region make sure it's a silent...

20. 15 Hot Plasmids from 2017

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    Blog Post

    ...utilize CRISPR Cas9 systems to defend themselves against bacteriophage infection, but some phages fight ...