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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 682 results for: RON

Showing: 321 - 340 of 682 results

  2. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...analysis window shows a green checked circle in front of the sample name. If there was a minor error during... error, you will see a red exclamation point in front of that sample. You can hover over the yellow or...line represents the cut site. Cutting and error-prone repair typically result in mixed sequencing bases...

  4. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ... Two-photon image of miRFP670nano3-expressing neurons in the neocortex of a living mouse. Image courtesy... and far-red genetically encoded indicators of neuronal activity. J Neurosci Methods 362, 109314 (2021...improved monomeric infrared fluorescent protein for neuronal and tumour brain imaging. Nat Commun 5, 3626 (...

  5. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    ... can be from one of the transgene’s endogenous introns or comprised of non-endogenous sequences, such ... A potential solution is to include an in-frame degron, or degradation sequence, upstream of the 5’ splice...Efficiencies of Dual AAV Approaches for Retinal Targeting. Front Neurosci 11: . https://doi.org/10.3389/fnins.2017.00503...

  6. Five Popular Model Organisms

    Type
    Blog Post
    ...research for over a century but was brought to the forefront by Thomas Hunt Morgan, who is known as the “father...work with as they can survive in a variety of environmental conditions, and double every 2 hours. Yeast ...Sydney Brenner brought it into the lab to study neuronal development (Brenner, 1973). C. elegans are small...

  8. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...determination By Mike Lacy Conventional cryogenic electron microscopy (cryo-EM) protocols typically require...in Xenopus nucleosomes and in complex with its chaperone NPM2, but the technique should be widely applicable...marking cell types in the nervous system By Ashley Waldron The mammalian nervous system is composed of myriad...

  9. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...and Ruddock., 2017), but requires an oxidizing environment to form. When expressed in the non-oxidizing ...cytoplasm. expression in the presence of molecular chaperones that help scFvs fold properly in the cytoplasm...and screened with in vitro assays to select for strong binders. Then the sequences for the variable domains...

  10. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ...adapted for video making.   Cycle One: The Ironing Board Control Center With the pandemic in full ...that included iPad and her laptop raised on an ironing board, giving it the perfect height (Fig. 2). We...to think of something else.   Figure 2: Ironing boards. Good as a WFH desk, and as a video control...

  11. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...neither the aptamer nor the small molecule are strongly fluorescent but the binding of an aptamer to its...investigate intracellular viral DNA dynamics in neuronal cells (Nilaratanakul et al., 2017) and levels ...Jonathan. "RNA fluorescence with light-up aptamers." Frontiers in chemistry 4 (2016): 29. PubMed PMID: 27446908...

  12. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...plasma HIV-1, they are at increased risk for other chronic diseases, including dementia, gut disorders, nerve...disease risk is attributed to latent HIV reservoirs, chronic inflammation, and negative metabolic effects of...in this publication at Addgene. Wang Z, Pan Q, Gendron P, Zhu W, Guo F, Cen S, Wainberg MA, Liang C. CRISPR...

  13. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ...are methylation sensitive. dnaJ Mutation in a chaperonin gene Increases the stability of certain expressed... fhuA (formerly tonA) ferric hydroxamate uptake, iron uptake receptor mutation. T1/T5 Phage resistance...not degraded, cell cannot methylate DNA lac Lac operon mutations Blue/white screening of clones   lacIq...

  14. Exploring Applications of the Bioluminescent HiBiT Tag

    Type
    Blog Post
    ...substrate generates a proportionate luminescent signal, strong enough to detect from proteins expressed at their...endogenous levels. HiBiT is compatible with both strong and weak promoters, and even modest endogenous ...compatible with both intra- and extracellular environments, making it effective for analyzing real-time...

  16. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ...from Yang et al. requires two gRNAs targeting two intronic regions and two repair templates in order to insert... allele requires you to target 2 loxP sites to intronic regions 0.5 - 0.8 kb apart. In the example below...CRISPR/Cas9-based generation of knockdown mice by intronic insertion of artificial microRNA using longer ...

  17. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ... was engineered from the cmt and cym bacterial operons. A chimeric transactivator, cTA, was created by...displays low promoter activity (~20% activity of the strong yeast promoter pTEF). As ethanol accumulates, it...sphingomonads and other Alphaproteobacteria. Appl Environ Microbiol. 2013 Nov;79(21):6795-802. doi: 10.1128...

  18. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ... E. coli or Salmonella, or any cells that bind strongly to nitrocellulose, over-incubation of plates can...pathways for bacteriophage-based therapeutics."Frontiers in Microbiology 7 (2016). PubMed PMID: 27536293...genomic and metagenomic screening." Applied and environmental microbiology 74.5 (2008): 1649-1652. PubMed ...

  19. Finding Your Perfect Job After University

    Type
    Blog Post
    ...very hard to speak with individuals who had very strong beliefs in traditional medicine, especially when... reliant on programming skills which are not my strong point. Working in industry: virology On completing...because it is an English speaking country with a strong economy and plenty of jobs in science at world ...

  20. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...e.g. an R01 from the NIH, in the United States) Strong letters of reference that attest to your talent...person makes time for you, as needed, this is a strong indicator that this person cares about your science... to finding out you've been doing something all wrong or that a reagent has not been convincingly demonstrated...
Showing: 321 - 340 of 682 results