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  1. Build Your CRISPR Vocabulary

    Type
    Blog Post
    ... and variable spacer sequences. The array is transcribed and processed into crRNAs, each including one...from Addgene!   RNA crRNA: “CRISPR RNAs” are transcribed from the CRISPR array and include a spacer and...

  2. Hot Plasmids - October 2022

    Type
    Blog Post
    ...called JEDI-2P. Using a custom, high-throughput screening platform to apply saturating mutagenesis on 21... 1: Summary of the approach and outcomes of the screen for improved genetically-encoded voltage indicators...

  3. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    ...showing the effectiveness of using TRUPATH as a screening technique. Lastly, the activity of both antagonists...plasmids are available as a collection, facilitating screening across the transducerome, to gain a more complete...

  4. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    ...characteristics of scientists in the work place as described by Sebastiano Massaro in his Nature Biotechnology...Introduction to Managing People for Scientists. Subscribe to the Addgene Blog to follow the entire series...

  5. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    ...find the vector that fits your experiments, I've described below some of the most frequently requested vectors... bacteria and a mammalian selection cassette to screen for positive transfected cells. The addition of...

  9. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    ...multicolor labeling is a powerful solution for discriminating between neurons for light microscopy-based ...expression level of XFPs, most likely by suppressing transcription. It is critical to express a minimal amount ...

  10. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    ...generally refer to them as the “interested public.” Subscribers to Scientific America would be considered the...scientists in (roughly) the same field, often through manuscripts, seminars, or posters. It is important to note...

  11. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ...ready-to-use viral preps   Improved cell-free transcription/translation system to examine biosynthetic pathways...pathways  By Gabrielle Clouse Recently, cell-free transcription/translation systems (also referred to as TX-...

  13. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    ...protein of interest. 4. Plasmids 101: Blue-White Screening Getting a lot of empty vector contaminants in ...Addgenie Jessica Welch explains how Blue-White screening could be a potential solution. 5. Cpf1: A New ...

  15. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...transduced using only protein. Read on for a description of the lab’s iTOP protein-only transduction method...transduction by osmocytosis and propanebetaine) to describe this method of protein transduction. Later studies...

  16. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    ...Coordinator’ came up at Abcam. Unlike other job descriptions for marketing positions posted on recruitment...would be able to carry out a yeast-2-hybrid library screen; instead I thought they would focus on “Has this...

  18. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...directly transformed into E.coli for plating, screening and generation of sgRNA. Generation of sgRNA libraries...Assembly and Other Long-Homology Based Cloning Methods Screen Plasmids by Colony PCR Addtional Resources on ...
Showing: 321 - 340 of 776 results