We narrowed to 763 results for: SCR

...Polymerases Restriction Endonucleases Reverse Transcriptases RNA Polymerases Other Enzymes Additional Resources...polymerases, RNA polymerases, DNA ligases, reverse transcriptases, and restriction enzymes. These enzymes are... cloning, isothermal amplification, reverse transcription of RNA into DNA, and dNTP synthesis. Additionally...Polymerases Restriction Endonucleases Reverse Transcriptases RNA Polymerases Other Enzymes DNA Ligases Addgene...165568 pOpen-NcoIR NcoI Return to top Reverse Transcriptases Addgene ID Plasmid Gene/insert 165546 pOpen-MMLV_RT...Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase (RNAse H deactivated by 3 mutations) 165556 ...Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase RNaseH - (lacking RNaseH domain) 165566 pOpen-TthRT...

...various CRISPR applications (such as visualize, screen, activate, and repress) on Thursday. We’d also ...from nickases to base editing to pooled library screening. It also has information for planning your CRISPR...

...Noninvasive assessment of gut function using transcriptional recording sentinel cells! 🎵 On the 11th day...gave to me: CRISPR activation and interference screens for primary human T cells🎵 On the 3rd day of CRISPR...

... bind transcriptional co-activators and modulate transcription. The list of p53 transcriptional targets...collection of plasmids for the p53 pathway. p53 is a transcription factor and tumor suppressor activated by cellular...p53 Gene List References Background p53 is a transcription factor and tumor suppressor activated by cellular...mutations interfere with p53’s ability to activate transcription, and they also have a dominant negative effect... receptor superfamily, member 10b E2F-1 E2F transcription factor 1 Fas Fas cell surface death receptor...

...promoter or cloning method of choice. ID Plasmid Description Promoter Cloning Method PI Synthetic Biology ...for creating plant expression vectors. ID Kit Description Cloning Type PI 1000000036 GreenGate Cloning ...different functional variants of Cas9. ID Plasmid Description PI Genome Editing Kits The following plasmid ...designed for genome editing in plants. ID Kit Description Gene Editing Type PI 1000000076 GoldenBraid 2.0...silencing suppressors, such as p19. ID Plasmid Description PI Additional Gene Silencing Resources A modular...physiological processes in plants. ID Plasmid Description PI Kits for Protein–Protein Interactions The ...protein–protein interactions in plants. ID Kit Description Cloning Type PI 1000000147, 1000000148, 1000000149...

...populate your “PubMed filter list” to the left of your screen. That’s it! Next time you log-in to your PubMed...Scientific Review Article Tips for Improving Your Next Manuscript Resources on Addgene.org Learn How to Order...

...used to increase or decrease expression of a gene. Screen: A library of gRNAs with a CRISPR nuclease target...cell population. The resulting mutant cells are screened for phenotypes of interest. Limitations of CRISPR...

... the REAL-Fast assembly method (Reyon et al., manuscript submitted), and the FLASH (Fast Ligation-based...Hockemeyer et al., Nat Biotechnol. 2011 ) TALE transcriptional activators in human cells ( Maeder et al., ...identify both dimeric TALEN and monomeric TALE transcriptional activator target sites and to simplify the ...sequences of interest for potential TALEN or TALE transcriptional activator target sites and then generates individual...for FLASH assembly. Links to additional pages describing these reagents are provided below: Joung Lab ...Vectors for REAL, REAL-Fast and FLASH TALE Transcriptional Activator Expression Vectors for REAL, REAL-Fast...

... Just click on the search bar at the top of the screen and type in what you’re looking for. After you ...know about new articles on the Addgene blog by subscribing to instant alerts below.  ...

...your experiment* Functional data, if the report describes a functional vector such as a channelrhodopsin...site as well as the target region, if the report describes a retrograde serotype An abstract that summarizes...

...individually isolated and expanded. The clonal cells are screened to identify hybridoma lines, each producing a ...antibodies include those directed against human transcription factors such as PAX7, PAX6, AP-2 alpha and ISLET...

...IGDCC3 (PUNC) Antibodies ID Recombinant Antibody Description Isotype Reactivity Recommended Applications PI...scientists worldwide. ID Recombinant Antibody Description Isotype Reactivity Recommended Applications PI...Neuroligin Antibodies ID Recombinant Antibody Description Isotype Reactivity Recommended Applications PI... Integrin Antibodies ID Recombinant Antibody Description Isotype Reactivity Recommended Applications Return...common cellular proteins. ID Recombinant Antibody Description Isotype Reactivity Recommended Applications Return...express plasmid-based antibodies. ID Plasmid Description Return to top...

...encoded on RNA. The genome includes a reverse transcriptase and an integrase to insert the viral genome ...plasmids that have inserts. ID Plasmid 5'LTR Description PI 1780 pBABE-neo largeTcDNA MoMLV Expression...cloning and gene expression. Select with puromycin or screen for GFP. David Bartel 32702 pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE...plasmid 12282 for the addition of WRE, a post-transcriptional regulatory element that increases export to...Gamma-Retroviral Packaging Plasmids ID Plasmid Description PI 8449 pUMVC Contains MoMLV gag and pol . Use...Gamma-Retroviral Envelope Plasmids ID Plasmid Description PI 1733 pCI-VSVG VSV-G recommended for the FELIX...

...Shapiro’s lab. After testing dCas9 fusions with transcriptional repressors, they found that fusing dCas9 to...to an Mxi1 repressor domain repressed transcription of HSP90 an essential C. albicans gene. Read the ...

...plasmid page. Check out the purple arrow in the screenshot below to see what I mean. Additional features...increase the length of the list (purple oval in the screenshot below). You can also use the "Search Table" box...

...expression plasmids. Large collections or kits are described below and not included in this table. For a more...search Addgene's entire collection. ID Plasmid Description Gene/Insert PI Plasmids Expressing Worm Genes...strains. CRISPR plasmids from John Calarco's lab as described in Heritable genome editing in C. elegans via ...genome-editing events. Developed in Craig Mello's lab and described in A Co-CRISPR Strategy for Efficient Genome Editing...also been developed by the Fire laboratory and described in Efficient Marker-Free Recovery of Custom Genetic...C. elegans and other nematodes. Wormbuilder - Describes methods to engineer the genome of the genetic ...

...rescue organization where I foster dogs and help screen dog adoption applications. My most recent foster...about Addgene in our "Inside Addgene" blog series Subscribe to the Addgene blog Resources on addgene.org:...

...including beta-catenin reporter M50 Super 8x TOPFlash, scramble shRNA for use as a negative control, and empty...rapidly in 2011, from zinc finger nucleases to transcription activator-like effector nucleases (TALENs). ...

...supporting the tools in our collection, such as blogs describing viral vector serotypes, or hundreds of Data Hub... to learn more about our latest developments? Subscribe to the Addgene newsletter. References Ayoubi,...

...communication between all members of a team. As described here in a 2012 Intuit blog post, a study coming...Introduction to Managing People for Scientists. Subscribe to the Addgene Blog to follow the entire series...