...provide cells with physical structure, separate chromosomes during mitosis, support transport of organelles...different CIB constructs to the plasma membrane or lysosomes (the earlier article also provided CIB constructs...constructs for targeting Golgi, ER, and endosome membranes) (Figure 4). The authors used SuperPLD to manipulate...A) OptoPLD is recruited to plasma membrane or lysosomes and its activity can be monitored by phosphatidic...enzymes. Conclusion We hope this post has illuminated some of the many tools available for controlling cell...

...genetic similarities with humans. However, rats have some key differences in physiology, intelligence, and...science with early research providing insight into some of the most significant and fundamental biological...in vivo studies. These attributes make frog eggs some of the most useful tools in developmental and biomedical...commonly used species of frog. (Liu et al., 2016). Some of the most popular tools for studying frogs include...world worked to characterize each gene’s function. Some key genetics research with Arabidopsis include understanding...

... dyes usually spread unidirectionally, there are some reports of bi-directionality. For predominately ...been modified and used as tools for tracing. With some engineering, RABV and HSV, but also others including...vectors have been widely used by neuroscientists for some time due to their advantageous characteristics for...retrograde and anterograde intracellular transport of some AAV serotypes, but overall with rather low efficiency...however, a few reports of trans-synaptic spreading of some AAVs, specifically AAV6 in the retrograde direction...monosynaptic and polysynaptic spreading. AAV vectors cover some of these attributes and their use has allowed for...

...c-MYC is the established model for reprogramming somatic cells. A common method for generating iPSCs to ...-excitable organge fluorescent protein (CyOFP1), some of the exciting properties of mCyRFP1 include a ...with HDR. Coselection efficiency is so high that some applications may permit working with edited “pools...see figure above). 3 Reasons The PSU Ladders Are Awesome Tools: They are comprised of 2 plasmids, pPSU1...looking for the production of ECT-DNA (extra-chromosomal telomeric DNA), which accumulates when T-loops... themselves against bacteriophage infection, but some phages fight back using CRISPR inhibitors. Several...

...We’re making some moves over here at Addgene...and they’re looping? What’s the deal with looping...level up in the lab, and we’ve started collecting some of our favorite (moving) images on a GIPHY page!...so well you need to express yourself. We have something for that too.   This could be you after...emotions (while you’re on a break from expressing some genes of interest).   Blugene is so excited...

... appropriately validated? This post will go over some of the general considerations you should review ...you may need to roll up your sleeves and perform some extra validation experiments yourself.  Goal of ...be concerned that your antibody is binding to something unexpected. This approach has high specificity...sites or alternative splicing that could lead to some form of the target protein to be present and recognizable...can be difficult to find epitope information for some antibodies, which makes it hard to ensure that you...

...students split into two groups for an in-depth look at some of the teams at Addgene. An introduction to plasmids...our Shipping Operations Coordinator: “Kory was awesome!”. The students also got to learn about intellectual... science and business or programming were new to some, who were surprised at “how much business there ...researchers, so Outreach Scientist Jessica Welch showed some highlights of his travels from 2015, including a...

...career paths. Check out Joanne's Reddit AMA Here are some concrete things you can do to develop those transferable...timelines Be a mentor in a formal program Start something (a journal, a science lunch club, a biotech club...division of labor Serve on a committee and do something big like plan a conference Recruit many supporters...departments want to be able to tell that you know about something and are passionate about it.   Maintain a content-useful...

...Flow cytometers however are highly versatile and some can accommodate up to 30 different colors. This ...the tag can alter the structure of the protein. In some cases this can lead to changes or a complete loss... lipids including those of the cell membrane. In some cases, this can mask epitopes such that immune complexes... complexes will no longer recognize them. Sometimes, users will combine methods and fix with formaldehyde...overview of flow cytometry for single cell analysis and some useful background information for setting up antibody...

...this blog is here to help. Here, we will outline some fundamental characteristics to consider when selecting...make your selection easier, as well as highlight some recent advancements within this field. Fundamental...be covalently attached to self-labeling proteins. Some common examples of these self-labeling proteins ... and its propensity to bind cellular components. Some dyes target specific organelles like mitochondria...technically cell-permeable, its use in live cells can sometimes result in cellular toxicity due to the permeation...

... their work. Thousands of people showed up; some with some really great signs. The Congressman for Albany...Harvard Medical School working on human artificial chromosomes. Montreal, QC, Canada 45.5017°N 73.5673°W – Vanessa...lab where the groundbreaking research happens, sometimes forgetting how to engage with friends, family,...science professionals and supporters. I'm raising some little scientists (as you can see in the picture...

...project. You’ve met someone whose career interests you or you want to pursue someone doing a job you wish...potential to alienate connections. If meeting with someone to ask about a job or a particular position at ...interest in a job. Ask openly to be connected to someone who can help you land that position such as a direct...

...expertise with lots of free beer.” He also mentions that some of the team will be moving to flats with gardens... in five years and hopefully I'll have worked something out!” We will get back to you on that, Tom! Connie...”. He also cooks and cleans with his family, “sometimes listening to audiobooks.” Church and his family... family. And as the school year winds down, take some time to have fun outside the lab! How does your ...

...every two weeks or every month is long enough that some experiments can get done and data analyzed between...scientists edit the same document may sound like something out of your nightmares, shared ownership of materials...the output that will come from the collaboration. Some things to figure out are timelines for experiments...communication within an existing collaboration, or start something new! Additional resources on the Addgene blog...

...drug downstream. In some cases, there may already exist a drug which targets some of the candidate genes...onset. These hits can reveal individual genes or sometimes even genetic pathways that are worth investigating...drug development. Due to the difficulty of drugging some targets – the dreaded ‘undruggable’ proteins – having...

... in scicomm has grown and now it feels like an awesome bionerd hobby but also a viable away-from-the-bench...was leaving a writing position and looking for someone who might take over. I like writing and science...communicator than a science writer these days. I do some public speaking and a bit of video work for webinars...after joining IDT when his boss was looking for someone to take over hosting them. Since then, webinars...

...thought we’d take some time to introduce you to the idea of the Freshers Fair and some of the great biological...scientific networks in their many years of study to come. Some Freshers Fairs can include fairground rides and ...know of a society or event which would benefit from some Addgene support? Please get in touch, as we aim ...

...parameters of CRISPR experimental design. Let’s dive into some structural biology! Major features First, we’ll ...is recognizing the PAM on DNA, the CTD is also sometimes called the PAM-interacting (PI) domain. The guide...al., 2013). As mentioned above, however, Cas9 sometimes tolerates mismatches in the next 8-10 bases after...7S4X), shown opaque (left) and transparent (right). Some nucleotides of the non-target DNA strand are not.../doi.org/10.1016/j.cell.2014.02.001 Osuka, S., Isomura, K., Kajimoto, S., Komori, T., Nishimasu, H., Shima...

...reporting on amazing women in science, or asking someone how and why they left academia. Humans are designed...research. Long story short, telling a good story is something that can be learned, and will serve you well in...yourself jumping backwards in time to explain something, rethink your structure. Develop your protagonist...but there are plenty of other options. Choose something with emotional significance that is likely to ...

...like we’re being buffeted by wind and waves and sometimes wonder if we can take another blow. Back in March... 2020 after rumors spread in our building that someone from a different company had tested positive for...as you are able... Thanks to everyone for being awesome during a tough time. We're all going to get through...access to quality materials and data. Addgene has some exciting new projects planned, and we look forward...