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We narrowed to 878 results for: SOM

Showing: 321 - 340 of 878 results
  1. Networking and Inspiring Young Scientists at Freshers Fairs

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    Blog Post
    ...thought we’d take some time to introduce you to the idea of the Freshers Fair and some of the great biological...scientific networks in their many years of study to come. Some Freshers Fairs can include fairground rides and ...know of a society or event which would benefit from some Addgene support? Please get in touch, as we aim ...
  2. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    ...like we’re being buffeted by wind and waves and sometimes wonder if we can take another blow. Back in March... 2020 after rumors spread in our building that someone from a different company had tested positive for...as you are able... Thanks to everyone for being awesome during a tough time. We're all going to get through...access to quality materials and data. Addgene has some exciting new projects planned, and we look forward...
  3. The Strength of Story Telling

    Type
    Blog Post
    ...reporting on amazing women in science, or asking someone how and why they left academia. Humans are designed...research. Long story short, telling a good story is something that can be learned, and will serve you well in...yourself jumping backwards in time to explain something, rethink your structure. Develop your protagonist...but there are plenty of other options. Choose something with emotional significance that is likely to ...
  4. Antibodies 101: Epitope Tags

    Type
    Blog Post
    ...The stress of finding a ‘good’ antibody is something we’ve all experienced. Finding an antibody that ...commonly available tags and their antibodies along with some pros and cons. FLAG FLAG is a synthetically derived...immunoprecipitation, western blot, and immunofluorescence with some success for protein purification as well. This tag...a protein may disrupt the biological function of some proteins. Protein folding issues, steric hindrances...
  5. What the HEK?

    Type
    Blog Post
    ... most chromosomes are represented by two to three copies, but several have four. No Y chromosome is present...gland is next to the kidney and is known to have some neuronal qualities.) While HEKs probably aren’t ...advantage to use the cell line and we will highlight some of those applications here.   Virus production HEKs...
  6. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    ...with the condition to reveal some “outstanding differences.” They now have some theories on the underlying...on rare diseases, offering them the chance to win some of the latest tools and technologies in life sciences...described in 1971,” McSweeney said. “But, it is still sometimes wrongly diagnosed as a seizure disorder.” In 2012...
  7. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    ...unwavering devotion to seeing your ideas become something tangible.  When you think of entrepreneurship ...multibillion dollar tech companies using only laptops and some server space. But what if you want to be an entrepreneur...heavy price tag, especially if you're working on something other than software.  If you are a graduate student...experiences founding and running miLab to describe some key lessons I learned while becoming a health science...
  8. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    ...genetic tools are available. While he recognizes that some beginners may have a tendency to shy away from ratiometric...he recommends they give it a shot. “Beginners sometimes think it’s complicated, but actually it is very...of success – whether we have really developed something useful [enough] that other people start using ...Check out our Hot Plasmid page, where we highlight some of the popular & newly available plasmids at Addgene...
  9. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    ... mentor, developed some strategies for finding mentors and, perhaps, asked someone to support your career...identify areas that need work or growth. Here are some ideas for making your meetings productive: Discuss... posse holds me accountable for attending and sometimes it is only the knowledge that they will be watching...
  10. CRISPR Guide

    Type
    Guide
    ...sequence, some base editing systems create a wide spectrum of single-nucleotide variants (somatic hypermutation...will introduce the basic mechanisms of CRISPR and some of the ways the CRISPR components are leveraged ...or inversions with two gRNA sites on the same chromosome. Most multiplex systems enable researchers to...cloning multiple gRNAs into a single plasmid — though some have achieved targeting in the double digits. These... currently no defined measure of fidelity, so sometimes they will be referred to as increased fidelity...amino acid residues to reduce off-target editing. Some mutations disrupt interactions between Cas9 and ... which inhibits both Cas12a and Cas3. Similarly, some Acr proteins are specific to a particular species...
  11. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ... of both anterograde (from soma to axon) and retrograde (from axon to soma) transport. Many rAAV vectors...viral replication (E4, E2a and VA). These are sometimes combined within the packaging plasmid. For a summary...restriction enzyme, Gibson Assembly, or Gateway. Some transfer plasmids may have limited restriction sites...genome at a specific site, the AAVS1 site on human chromosome 19. This integration is mediated by proteins ...typically processed into a double-stranded circular episome which can concatemerize, producing high molecular...molecular weight structures that are maintained extrachromosomally. Random integration of rAAV genomes in non-homologous...majority of rAAV DNA is thought to be maintained in episomes or concatemers. This avoids the effects local ...
  12. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    ...students! After a course coordination meeting with some other professors, I went home thinking about how...When I got home, I saw my daughters playing with some cards and I had a quick flashback to time spent ... most of the concepts required by the course and some were so enthusiastic that they took the cards and...gamification” will lead to new cures for disease someday! Accessing and modifying BUILD YOUR PLASMID You...
  13. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    ... news and there’s bad news. The bad news is that some of tubes’ o-rings are, in fact, permeable to CO2...information in mind if you’re planning on shipping something on dry ice or if you’re receiving samples on dry... dry ice - it may prevent you from seeing some unexpected results. Order Ready to Use Viral Preps from... can affect the pH of the sample, which may have some effect on the contents of the sample. For example...
  14. Management for Scientists: Delegating is Key

    Type
    Blog Post
    ...take responsibility if things go wrong. Here are some important criteria for delegating tasks appropriately...Specify desired results. Are you delegating to someone with sufficient skills and experience? Do you think... think you are giving this responsibility to someone who can do the job. It is good to delegate so the...and resources needed for the assignment? Setting someone up for failure by delegating is a bad idea. How...
  15. Advice for Scientists Starting a Lab

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    Blog Post
    ...Genevive Bjorn's "Ready, Set, Hire" on Nature Jobs for some quick advice and resources for hiring research staff... and dealing with email can be half of my day sometimes.” He points out that the copious amounts of email...postdoc may require different types of mentoring. Some mentees prefer lots of advice and micromanagement...The Howard Hughes Medical Institute (HHMI) offers some great resources for Early Career Scientists, including...
  16. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

    Type
    Blog Post
    ... of these “variants of unknown significance” (sometimes called “VUS”) function is unknown. How can we ... rare variants that haven’t been characterized? Some have developed exciting computational algorithms...acid change based on maps of protein structure. Sometimes this provides a great deal of clarity. But, often...genes: High-throughput Phenotyping of Lung Cancer Somatic Mutations. Berger AH, Brooks AN, Wu X, et al. Cancer...
  17. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...that didn’t get answered live - but he kindly took some time to address all your unanswered questions via...be oversized even before considering the need for some manner of polyA. In this scenario, you may be best...adopting ddPCR, which does not a standard curve and somewhat more reliable quantitation. Differences in sample...VAST) experiments using PHP.eB in the brain give some insight into codelivery efficiency at different ...
  18. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    ...manufacturer, lot number, and expiration date somewhere you can easily check it whenever you use the antibody...freeze-thaw cycles, as they’ll degrade your antibody.  At some point in your long and illustrious lab career, you... with other chemicals and biological structures, some of which you may need in your experiment.  If you... to buffers, can bind to amine groups present in some conjugates, blocking the conjugation reaction. Many...
  19. From Lab Bench to Leadership: Transitioning into Management as a PhD

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    Blog Post
    ...myself would take less time than the seemingly burdensome task of explaining the steps to a lab assistant...lab scientist, I worked with a diverse team where some members thrived on independence, while others required...dynamics. I experienced this firsthand when it took me some time to adapt to the communication style of a new...do this while changing industries and positions. Some people discover they truly enjoy management and ...
  20. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ... all the Cas enzymes you know and love, and even some you’ve likely never heard of. Each entry displays...adjacent PAM or protospacer-flanking sequence (PFS) in some cases. The resource displays these requirements ... orientation relative to the gRNA (5’ or 3’). In some cases, there is no requirement, which is also documented...CasPEDIA.   Helpful resources and information For some researchers, the previous information may be all...
Showing: 321 - 340 of 878 results