We narrowed to 466 results for: Sele
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TypeBlog Post...asked whether this step can be skipped or whether selecting colonies and performing Sanger sequencing will...
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Finding nucleic acids with SHERLOCK and DETECTR
TypeBlog Post...ability to rapidly detect nucleic acids with high selectivity and sensitivity from patient samples. Find the... -
CRISPR-mediated Plant Base Editors
TypeBlog Post...GC’ sequence context. If this cannot be avoided, select the target AID version. Expect very low to nil ... -
Plasmids 101: Codon usage bias
TypeBlog Post... PMC2364656. 2. Dittmar, Kimberly A., et al. "Selective charging of tRNA isoacceptors induced by amino‐acid... -
Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design
TypeBlog Post...balanced with predicted off-target activity when selecting guides. Guides were delivered as a pooled library... -
Transferable Skills Guide: Creativity
TypeBlog Post...will fight more adamantly for their solution), selecting a devil’s advocate is a good option for any meeting... -
Using Video to Share Your Science: We Share Science
TypeBlog Post...research for single or multiple disciplines – you can select the disciplines and the years. Which leads to the... -
Getting the Most from Your Lentiviral Transduction
TypeBlog Post...general approach for using lentivirus, infect and select, seems simple, in actuality, many find using lentivirus... -
Data Freedom: The Expansion of Data Sharing in Research Publications
TypeBlog Post...someone other than the actual author. Instead, selected images are said to be representative or the statistical... -
Genomic Deletions in Mammalian Cell Lines
TypeCollection... and SpCas9, but does not contain markers for selection 1 . Other constructs may be utilized, such as ...ID 48139), which include GFP and puromycin as selectable markers, respectively, or constructs in which...Screening CRISPR/Cas9 Clones for Deletions and Clone Selection For suspension cells, transfer all clones to a...conditions optimized on the bulk cells (see step 6 ). Select the clones identified with the desired deletion...onto LB agar plates with the relevant antibiotic. Select multiple colonies, mini-prep each one, and subject...initial screen ensures that the correct clone was selected and reproducibility of results. To evaluate the... -
New Optogenetic Tools for Cytoskeleton and Membrane Control
TypeBlog Post...directed-evolution methods, the activity-based selection in mammalian cells used here could be an attractive... -
Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping
TypeBlog Post...requires less biotin, and allows scientists to more selectively target fusion proteins to specific locations ... -
Fluorescent Proteins 101: When GFP lets you down
TypeBlog Post...performing probe, it is the first choice when selecting a genetically encoded fluorescent tag. There are... -
Antibodies 101: Introduction to Immunofluorescence
TypeBlog Post...carefully review your options. If possible, try to select a couple of antibodies that seem promising to compare... -
3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells
TypeBlog Post...insertions/deletions, therefore making those alleles useless for our studies. If one now also takes into account... -
March for Science
TypeBlog Post...information for your senators all you need to do is select your state. State Legislatures: To find the website... -
Site Directed Mutagenesis by PCR
TypeBlog Post...from the parental plasmid provides a mean for selecting for transformants which have taken up the mutagenized... -
Evolution of Lab Techniques
TypeBlog Post...still be used to screen multiple colonies before selecting a smaller subset to sequence. Sequencing gels ... -
Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons
TypeBlog Post.... Cre production drives recombination and XFP selection, followed by Cre self-excision. These constructs... -
Plasmids 101: Common Lab E. coli Strains
TypeBlog Post...worked hard designing your plasmid – you carefully selected the optimal promoter for your gene of interest...