We narrowed to 472 results for: Sele
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TypeCollection...Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter Selectable Marker PI... Promoter Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert Promoter Selectable Marker PI ...Publication Zebrafish ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Do you have suggestions...
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Plasmids 101: Codon usage bias
TypeBlog Post... PMC2364656. 2. Dittmar, Kimberly A., et al. "Selective charging of tRNA isoacceptors induced by amino‐acid... -
Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design
TypeBlog Post...balanced with predicted off-target activity when selecting guides. Guides were delivered as a pooled library... -
Finding nucleic acids with SHERLOCK and DETECTR
TypeBlog Post...ability to rapidly detect nucleic acids with high selectivity and sensitivity from patient samples. Find the... -
CRISPR-mediated Plant Base Editors
TypeBlog Post...GC’ sequence context. If this cannot be avoided, select the target AID version. Expect very low to nil ... -
Using Video to Share Your Science: We Share Science
TypeBlog Post...research for single or multiple disciplines – you can select the disciplines and the years. Which leads to the... -
Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)
TypeBlog Post...region of your final plasmid or those you are selecting for further assemblies. This will help confirm... -
Antibodies 101: The Four ELISAs and When to Use Them
TypeBlog Post...Addgene blog Antibodies 101: ELISAsAntibodies 101: Selecting the Right Antibody Antibodies 101: Polyclonal ... -
Hot Plasmids Spring 2024
TypeBlog Post...Addgene offers vectors for CROPseq-multi using selection by Puromycin, Zeocin®, or nuclear expression of... -
Antibodies 101: Reading a Flow Plot
TypeBlog Post...above. There’s many variations in how one can gate, select, and analyze data. But now that you can read a ... -
Viral Vectors 101: Preparing Pooled Libraries
TypeBlog Post...asked whether this step can be skipped or whether selecting colonies and performing Sanger sequencing will... -
Transferable Skills Guide: Creativity
TypeBlog Post...will fight more adamantly for their solution), selecting a devil’s advocate is a good option for any meeting... -
Getting the Most from Your Lentiviral Transduction
TypeBlog Post...general approach for using lentivirus, infect and select, seems simple, in actuality, many find using lentivirus... -
Data Freedom: The Expansion of Data Sharing in Research Publications
TypeBlog Post...someone other than the actual author. Instead, selected images are said to be representative or the statistical... -
Genomic Deletions in Mammalian Cell Lines
TypeCollection... and SpCas9, but does not contain markers for selection 1 . Other constructs may be utilized, such as ...ID 48139), which include GFP and puromycin as selectable markers, respectively, or constructs in which...Screening CRISPR/Cas9 Clones for Deletions and Clone Selection For suspension cells, transfer all clones to a...conditions optimized on the bulk cells (see step 6 ). Select the clones identified with the desired deletion...onto LB agar plates with the relevant antibiotic. Select multiple colonies, mini-prep each one, and subject...initial screen ensures that the correct clone was selected and reproducibility of results. To evaluate the... -
Fluorescent Proteins 101: When GFP lets you down
TypeBlog Post...performing probe, it is the first choice when selecting a genetically encoded fluorescent tag. There are... -
3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells
TypeBlog Post...insertions/deletions, therefore making those alleles useless for our studies. If one now also takes into account... -
March for Science
TypeBlog Post...information for your senators all you need to do is select your state. State Legislatures: To find the website... -
Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping
TypeBlog Post...requires less biotin, and allows scientists to more selectively target fusion proteins to specific locations ... -
New Optogenetic Tools for Cytoskeleton and Membrane Control
TypeBlog Post...directed-evolution methods, the activity-based selection in mammalian cells used here could be an attractive...