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We narrowed to 329 results for: cloning

Showing: 321 - 329 of 329 results

  1. Addgene's eBook Collection

    Type
    Guide
    ...nomenclature, and uses to common promoters and cloning techniques! Close About Overlay Select CRISPR 101...

  2. Sequencing Primers

    Type
    Guide
    ...forward primer pAd-CMV GCTAGAGATCTGGTACCGTC For cloning sites after SalI in pAd-CMV vector pBABE 3' ACCCTAACTGACACACATTCC...

  3. CRISPR Guide

    Type
    Guide
    ...researchers to target anywhere from 2–7 genetic loci by cloning multiple gRNAs into a single plasmid — though some... the desired edit experimentally and to isolate clones containing the desired edit (see: Validate genetic...designed in silico and synthesized (Figure 8A), then cloned in a pooled format into lentiviral transfer vectors...PAM sequence in the genomic DNA. This sequence is cloned into a gRNA expression plasmid but does NOT include...

  4. Optogenetics Guide

    Type
    Guide
    ...light-inducible dimerization tags (TULIPs), plasmids allow cloning of protein coding sequences with GFP-LOVpep, cpPDZ...

  5. General Transfection

    Type
    Protocol
    ...function. We specifically use this protocol with a subclone of HEK293T cells optimized for viral production...Workflow Timeline Day 0: Seed HEK293T cells (or a subclone of HEK293T optimized for viral production) Day...

  6. Immunology Research Plasmids and Resources

    Type
    Collection
    ...MXS-chaining kit includes chaining vectors with multiple cloning sites and standardized building blocks: 14 different...kinases and kinase-related open reading frames (ORFs) cloned into a retroviral vector which adds a myristoylation...

  7. AAV ddPCR Titration

    Type
    Protocol
    ... (x 3) Reagents Molecular Biology Grade Water, Hyclone, SH30538.02 GeneAmp 10X PCR Buffer, Applied Biosystems...

  8. Lentivirus ddPCR Titration

    Type
    Protocol
    ...Millipore, TR-1003-G Molecular Biology Grade Water, Hyclone, SH30538.02 ddPCR Supermix for Probes no dUTP, ...

  9. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...applications. A substantial number of these have been cloned, expressed and validated in recombinant form. The...
Showing: 321 - 329 of 329 results