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We narrowed to 326 results for: cloning

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Showing: 321 - 326 of 326 results

  1. CRISPR Guide

    Type
    Guide
    ...researchers to target anywhere from 2–7 genetic loci by cloning multiple gRNAs into a single plasmid — though some... the desired edit experimentally and to isolate clones containing the desired edit (see: Validate genetic...designed in silico and synthesized (Figure 8A), then cloned in a pooled format into lentiviral transfer vectors...PAM sequence in the genomic DNA. This sequence is cloned into a gRNA expression plasmid but does NOT include...

  2. Guide to Using Pooled Libraries

    Type
    Guide
    ...cDNA is synthesized, ligated to adapters, and then cloned into a given vector. They are useful for gain-of-function...

  3. AAV ddPCR Titration

    Type
    Protocol
    ... (x 3) Reagents Molecular Biology Grade Water, Hyclone, SH30538.02 GeneAmp 10X PCR Buffer, Applied Biosystems...

  4. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...applications. A substantial number of these have been cloned, expressed and validated in recombinant form. The...

  5. Lentivirus ddPCR Titration

    Type
    Protocol
    ...Millipore, TR-1003-G Molecular Biology Grade Water, Hyclone, SH30538.02 ddPCR Supermix for Probes no dUTP, ...

  6. Optogenetics Guide

    Type
    Guide
    ...light-inducible dimerization tags (TULIPs), plasmids allow cloning of protein coding sequences with GFP-LOVpep, cpPDZ...
Showing: 321 - 326 of 326 results