We narrowed to 327 results for: cloning

...cDNA is synthesized, ligated to adapters, and then cloned into a given vector. They are useful for gain-of-function...

...researchers to target anywhere from 2–7 genetic loci by cloning multiple gRNAs into a single plasmid — though some... the desired edit experimentally and to isolate clones containing the desired edit (see: Validate genetic...designed in silico and synthesized (Figure 8A), then cloned in a pooled format into lentiviral transfer vectors...PAM sequence in the genomic DNA. This sequence is cloned into a gRNA expression plasmid but does NOT include...

...MXS-chaining kit includes chaining vectors with multiple cloning sites and standardized building blocks: 14 different...kinases and kinase-related open reading frames (ORFs) cloned into a retroviral vector which adds a myristoylation...

...function. We specifically use this protocol with a subclone of HEK293T cells optimized for viral production...Workflow Timeline Day 0: Seed HEK293T cells (or a subclone of HEK293T optimized for viral production) Day...

... (x 3) Reagents Molecular Biology Grade Water, Hyclone, SH30538.02 GeneAmp 10X PCR Buffer, Applied Biosystems...

...applications. A substantial number of these have been cloned, expressed and validated in recombinant form. The...

...Millipore, TR-1003-G Molecular Biology Grade Water, Hyclone, SH30538.02 ddPCR Supermix for Probes no dUTP, ...