We narrowed to 364 results for: gne

1. How to Design Your gRNA for CRISPR Genome Editing

   Type

   Blog Post

   ...but it is important that your gRNA sequence is designed to be highly active and reduce off targets. For...

2. CRISPR 101: Epigenetics and Editing the Epigenome

   Type

   Blog Post

   ...certainly not the only CRISPR-based technology designed to alter gene expression. Fusing dCas9 to a transcriptional...

3. Viral Vectors 101: AAV Serotypes and Tissue Tropism

   Type

   Blog Post

   ...., Schaffer, D. V., & Karpova, A. Y. (2016). A Designer AAV Variant Permits Efficient Retrograde Access...

4. Technique: Probe Phage Genomes for Host Binding Proteins

   Type

   Blog Post

   ...ends) and ligate into a bluntly digested vector designed for protein overexpression in E. coli, such as...

5. CRISPR Library Amplification

   Type

   Protocol

   ...library during amplification. This protocol is designed to be as general as possible but note that individual...than Nanodrop, although most protocols have been designed with Nanodrop as the quantification method. If...

6. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

   Type

   Blog Post

   ... Ghanta KS, Doksin GA, Mir A, Krishnamurthy PM, Gneid H, Edraki A, Watts JK, Sontheimer EJ, Mello CC (...

7. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

   Type

   Blog Post

   ... amplified a 500-600 bp product. The primers I designed (listed above) worked well and I got clear bands...

8. Live and Let Dye: Self-Labeling Protein Tags

   Type

   Blog Post

   ... such as with substrate-linked PEG, biotin, or magnetic beads. In one innovative example, the Zhang lab...

9. Deep Dive: Statistical Tests (Comparisons)

   Type

   Blog Post

   ...Correction and the Benjamini-Hochberg procedure are designed to reduce false positive rates and control for...

10. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type

    Blog Post

    ...Dewari et al. 2018). Using the first crRNA we designed for ~250 genes, we have had about 30% tagging ...

11. Antibodies 101: Flow Cytometry

    Type

    Blog Post

    ...based on intensity. Higher intensity events are assigned to higher channels. In a typical assay a user ...

12. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type

    Blog Post

    ...destroy most PA-FPs, but, in 2015, the Looger Lab designed 2 variants of EosFP that can withstand these conditions...

13. Antibodies 101: Designing Your First Flow Panel

    Type

    Blog Post

    ...avoid or reduce it whenever possible. Finally, we designed a simple panel to analyze transduced HEK 293T ...

14. CRISPR 101: Multiplex Expression of gRNAs

    Type

    Blog Post

    ...also have the option of including a previously-designed tyr gRNA, which causes hypopigmentation, thus ...

15. CRISPR 101: Cytosine Transversion Editors

    Type

    Blog Post

    ...Zhou, R., Iyer, S., Garcia, S. P., Miller, B. R., Langner, L. M., Grünewald, J., & Joung, J. K. (2021). CRISPR...

16. A Needle in a Base-Stack: Cas9 Structural Biology

    Type

    Blog Post

    ...Have you ever designed a CRISPR guide RNA and wondered why it is limited to only 20 bases, or why it’...

17. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type

    Blog Post

    ...-targeting, but currently no software has been designed for this purpose. This second area is extremely...

18. Deep Dive: qPCR

    Type

    Blog Post

    ...References and Resource section. Validation Once designed, your primer and probe set needs to be validated...

19. CRISPR 101: Cytosine and Adenine Base Editors

    Type

    Blog Post

    ...C. A., Garcia, S. P., Iyer, S., Miller, B. R., Langner, L. M., Hsu, J. Y., Aryee, M. J., & Joung, J. K...

20. Using AAV for Neuronal Tracing

    Type

    Blog Post

    ...., Schaffer, D.V., and Karpova, A.Y. (2016). A designer AAV variant permits efficient retrograde access...