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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 588 results for: ret

Showing: 321 - 340 of 588 results

  1. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ... technology has been used to optimize protein secretion and expression in the biotech workhorse yeast ...Modular Toolkit for Generating Pichia Pastoris Secretion Libraries.” ACS Synthetic Biology, vol. 6, no....

  2. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ... proper behavioral readout that allows you to interpret the results of your experiment. Like many things...optogenetics has already been proven. Therefore, a concrete, well defined, and robust behavioral output is...

  3. Arf GTPase Family

    Type
    Collection
    ...Mammalian (pEGFP-C1) GEF Fbxo8 (FBX8) 26269 319 Gateway Return to top The content for this page was generated ...

  4. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...editors have an activity window restricted to the stretch of ssDNA created by Cas9 binding. Pro tip! In CRISPR...editors (calling them GBEs), Xueli Zhang’s lab, returned with a unique approach to improving C → G editing...

  5. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    .... Other techniques, like electron microscopy and FRET, can offer more information on these regions.   ...SuperFi-Cas9, a high-fidelity variant that still retains high cleavage efficiency (Bravo et al., 2022). ...

  7. Deep Dive: qPCR

    Type
    Blog Post
    ...when using fluorescent probe-based chemistry - theoretically, up to five or six reactions in a single well.... Epub 2009 Feb 26. PMID: 19246619.Downey N. Interpreting melt curves: An indicator, not a diagnosis. ...

  8. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...Interspaced Short Palindromic Repeats, in Pubmed returned 728 articles (3/12/2014). With so many options...g3.112.005439  Cong, L., Ran, F. A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D., Wu, X., Jiang, W.,...

  9. Technical Design of a Western Blot

    Type
    Blog Post
    ...has both high binding capacity and high protein retention. It comes in a variety of pore sizes and protein...with high binding capacity, though it has lower retention after binding and washes. It’s less sturdy and...

  10. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ... rule of thumb, the more strictly you want to interpret your blot’s data, the more time you’ll want to...starting a western blot, think about how you’ll interpret the data and what level of accuracy you’ll need...

  11. Deisseroth INTRSECT Collection

    Type
    Collection
    ...transgenic recombinase-expressing animal lines and retro-grade expressing viruses delivering additional recombinases...

  14. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...promoter sequence. Should one of these random stretches of DNA contain an enhancer, it will activate its...GMAP-compatible backbones for lentivirus LV 1-5 and retrovirus RV 2-5 were constructed. The authors then used...

  19. Call for Guest Bloggers

    Type
    Blog Post
    ...protein purification Replica plating Anti-CRISPR Retroviral vectors Career development for scientists A new...
Showing: 321 - 340 of 588 results