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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 731 results for: Sis;

Showing: 681 - 720 of 731 results

  14. Water Bath Protocol

    Type
    Protocol
    ...allow the temperature to stabilize. Use water resistant markers, such as permanent markers, to label your...water bath, be sure to use a marker that is also resistant to chemicals such as ethanol. Writing on the tops...

  16. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...generate predictions of these outcomes. Rigorous analysis is essential to ensure off-target events are below...

  17. A Control for All Seasons

    Type
    Blog Post
    ... dead or dying cells so that you can base your analysis on living cells only (for example, propidium iodide...

  27. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ... cells (for example, plasmid with a puromycin resistance cassette, such as PX459). After you co-transfect...

  28. DNA Quantification

    Type
    Protocol
    ...such as TE). You will get much more accurate and consistent readings from DNA in a buffered solution. Reference...

  29. Protocol - How to Design Primers

    Type
    Protocol
    ...Reaction Plasmid Cloning by PCR Agarose Gel Electrophoresis Primer Design for PCR Oligonucleotide primers...

  32. Ligation Independent Cloning

    Type
    Protocol
    ...linearized vector from the reaction mixture by gel electrophoresis followed by gel purification . The cut vector...

  34. Pipetting Protocol

    Type
    Protocol
    ...multiple “stops” on the plunger, where you feel resistance while pressing down on the pipette. Stop at the...

  37. Lentivirus ddPCR Titration

    Type
    Protocol
    ... Benzonase and harvest cells Day 4+: ddPCR and analysis Equipment Class II, Type A2 Biological Safety ...

  38. Molecular Biology Reference

    Type
    Guide
    ...populations, an antibiotic resistance gene (i.e., a gene whose product confers resistance to ampicillin) is included...providing a benefit to the host, such as antibiotic resistance. This benefit can be context-dependent, and thus...of restriction enzymes, DNA ligase, and gel electrophoresis allowed for the ability to move specific fragments...includes a DNA replication origin, an antibiotic-resistance gene, and a region in which exogenous DNA fragments... origin of replication ( ori ), an antibiotic-resistance gene, and at least one unique restriction enzyme...plasmids are convenient and easy to use. Antibiotic Resistance Gene Allows for selection of plasmid-containing... important to distinguish that the antibiotic resistance gene is under the control of a bacterial promoter...

  39. CRISPR Guide

    Type
    Guide
    ...including resistances to chemotherapy drugs and toxins, cell viability, and tumor metastasis. One of the...editing systems include targeted AID-mediated mutagenesis (TAM) and CRISPR-X , in which Cas9 is fused to...nsertion of T ransposable E lements by G uide R NA- A ssisted T argeting), developed by Samuel H. Sternberg’s...large scale edits. PASSIGE ( P rime- E diting- A ssisted S ite- S pecific I ntegrase G ene E diting), developed...desired phenotype. The ease of gRNA design and synthesis, as well as the ability to target almost any genomic...simplest dCas9-based activators and repressors consist of dCas9 fused directly to a single transcriptional...than other activators/repressors due to their persistence and heritability. Not all modifications are stable...

  40. Lentiviral Vector Guide

    Type
    Guide
    ...markers, such as the puromycin resistance gene, conferring antibiotic resistance to infected host cells. When...Gateway cassette containing the ccdB gene, a ccdB-resistant strain is necessary. For more information on cloning...led to the development of multiple genome-wide analysis pipelines to assess viral integration (see Kim...information encoded by that genome. Antibiotic resistance is not the only type of selectable marker. Fluorescent...replication-competent lentiviral vectors The potential for oncogenesis The potential for generation of replication-competent... of the full-length virus. The potential for oncogenesis is largely based on whether or not the specific... for oncogenes should be made on a case-by-case basis. As with most experiments, infection risks occur...
Showing: 681 - 720 of 731 results