Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 1,046 results for: FER

Showing: 851 - 900 of 1046 results

  1. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    ...rumors spread in our building that someone from a different company had tested positive for COVID-19 (it turned...studying the coronavirus. Addgene’s Technology Transfer Team also moved fast to ensure that the majority...

  2. Plasmid Cloning by PCR

    Type
    Blog Post
    ...restriction enzymes that can both function in the same buffer, as this will save time later In our example,...Resources on Addgene.org Check out Our Plasmid Reference Pages Deposit Your Completed Plasmids Find the...

  3. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    ...translocations. It also provides researchers with many different ways to control gene expression. Get the full ...Joanne Kamens explains what career coaches do and offers up some career coach suggestions. 8. A Match Made...

  4. Hot Plasmids - January 2023

    Type
    Blog Post
    ...Facility Isotype IgG2a Differences:  Distinct variable domains that recognize different epitopes on GFP Monoclonal...

  8. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...have made it easier to track translation in two different ways: by monitoring the first round of translation...various cell types and physiological states. References 1. Ingolia, Nicholas T., et al. “Genome-wide ...

  9. 5 Great Apps for Lab Life

    Type
    Blog Post
    ...restriction enzymes since the instructions use NEB buffers. Nonetheless, if you are setting up a double digest... provides a list of directions including which buffers to use and that indicates if a sequential digest...

  10. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ...inactive state. The dynamic range, defined as the difference between the lowest and highest level of “output...various scientific or engineering applications.   References Refactoring and optimization of light-switchable...

  11. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    ...of prominent members of the bioscience and tech transfer communities who help guide and promote Addgene... of how miRNAs regulate their targets and what confers specificity in the miRNA regulatory process. This...

  12. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    ... proteins whose excitation and emission spectra differ sufficiently for them to be imaged in distinct ...fluorescent channels on the microscope. With the proliferation of fluorescent proteins in recent years, there...

  15. Antibodies 101: ChIP

    Type
    Blog Post
    ... the cutting. While sonication is usually the preferred method, enzymatic digestion can be appropriate...protocols when embarking on your ChIP journey!  References and Resources More resources on Addgene’s blog...

  16. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    ... web of opportunities that the classroom cannot offer. Furthermore, companies across most industries tend...helps you find internships and scholarships in different subject areas!    2. Resume and cover letter...

  19. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ...and SV40 promoters, are always active and thus referred to as constitutive promoters. Others are only ...Experimental System Because transcription machinery differs between cell types or organisms, promoters are ...

  20. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...the system and the flexibility to address many different types of research questions. Are you ready to ...experiments? Find EvolvR plasmids at Addgene! References Gaj, Thomas, and Pablo Perez-Pinera. "The continuously...

  22. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    ... and precisely identifies plasmid features that differ by only a few nucleotides, such as the fluorescent...static image file (PNG, see above) for your own reference or to paste directly into your notebook. This ...

  23. Hot Plasmids - May 2022

    Type
    Blog Post
    ...peptide. By combining three different localization peptides with four different fluorescent proteins, and...

  24. The time and cost required to make a plasmid

    Type
    Blog Post
    ...This post was originally written by Jennifer Tsang and updated by Rachel Leeson. Have you ever wondered...period. For ease of calculations*, let’s split the difference and put scientific inflation at… roughly 34.9%...

  26. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...us develop these resources further. Feel free to offer your comments and suggestions, and even to submit...submit a guest blog entry of your own! References 1. Bernard, P. “Positive Selection of Recombinant DNA ...

  27. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    ...function of the protein and pdDronpa are preserved. References 1. Zhou, Xin X., Linlin Z. Fan, Pengpeng Li, ...28232577. Figures 2-4 were modified from this reference. 2. Regot, Sergi, Jacob J. Hughey, Bryce T. Bajar...

  31. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ...virtual bookshelf. Pro tip The “cited by” and references sections of a PubMed entry are great places to...This library can also be synced with a Mendeley reference manager account. Pro tip Sign up for weekly email...

  35. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    ...species, like S. aureus and N. meningitidis require different PAM sites than SpCas9, in this case, NNGRRT and...is working for you in the comments section! References Hu, Johnny H., et al. "Evolved Cas9 variants ...

  36. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...explaining CRISPR technology to 4th graders, check out Jennifer Doudna’s new book A Crack in Creation. Making... the target DNA strand, and then activate two different endonuclease active sites. Fortunately, there ...

  37. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...against improper, random recombination into a different locus. Correctly recombined cells will not contain...should increase. New genome engineering tools also offer hope for the creation of new knockout animal models...

  38. Academic vs. Industry Postdocs

    Type
    Blog Post
    ... the process is similar to academia. The only difference is that your paper will be reviewed by an internal...LinkedIn connections and other contacts to get that referral and find out more about the company’s program....

  40. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ...expertise with our district middle school was a very different experience since, unlike elementary schools, middle...such a valuable extension to our curriculum and offered engaging examples of how understanding genetics...

  41. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...comes down to the researcher’s familiarity with different systems.  Using Cas9-gRNA RNPs in your experiments...include genome engineering and microbiology.     References 1. Chen, Sean, et al. "Highly efficient mouse...

  42. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ...truth, people are incredibly varied in how they prefer to consume information. Personally, I would much...outcome to the limitations and potential of your preferred platform before you start can save a lot of trouble...

  43. CRISPR Antimicrobials

    Type
    Blog Post
    ...mixed population, represented here by cells of different colors, specifically targets bacteria with the...ground in the fight against bacterial superbugs. References 1. Citorik, Robert J., Mimee, Mark, and Timothy...

  45. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...Red-shifts the wavelength (yellow derivatives) A206K Interferes with dimer interface (monomeric derivatives) ...earliest uses of GFP. Fluorescence resonance energy transfer (FRET): This is used to study the interactions...

  47. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    ...existing comprehensive mutagenesis methods, PFunkel offers the best combination of library coverage, mutational...mutagenesis? How would you use it in your research? References 1. Natarajan, Chandrasekhar, et al. "Epistasis...

  48. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...similar properties to Broccoli despite binding to a different small molecule. Notably, like Broccoli, Red Broccoli...reports on the next additions to aptamer soup! References Alam, Khalid K., et al. "A fluorescent split ...

  50. New Antibody Animation!

    Type
    Blog Post
    ...are, their role in the immune response, and the different antibody isotypes found in mammals.      Subscribe...
Showing: 851 - 900 of 1046 results