We narrowed to 1,052 results for: FER

...non-specific binding). Historically, glutathione-S-transferase (GST) fusion proteins have been immobilized on...resonance (Biacore, GE Life Sciences) or bio-layer interferometry (Octet, Pall ForteBio). Tips for using pMal-T-Avi-His...

...but when it came time to add author details a different person in the lab wanted to be the first author...scientists in SciComm careers Check out our series on transferable skills ...

...rumors spread in our building that someone from a different company had tested positive for COVID-19 (it turned...studying the coronavirus. Addgene’s Technology Transfer Team also moved fast to ensure that the majority...

...actually from a completely unexpected finding in a different experiment, this tool was developed because a ...doing and sharing science in the first place?   References and Resources More resources on the Addgene blog...

...whether any given antibody is available through different companies (Bandrowski et al., 2016). It’s also... the antibody sharing landscape as well.     References Andrews NP, Boeckman JX, Manning CF, Nguyen JT...

...the issue is not unique to IHC.  References and Resources References Goodpaster T, Randolph-Habecker J...

...Dr. Polka made R-body mutants that respond to different pH’s and some that are fused to fluorescent proteins...your target gene, activation and repression of different genes in a single cell, and localization of a ...

...restriction enzymes that can both function in the same buffer, as this will save time later In our example,...Resources on Addgene.org Check out Our Plasmid Reference Pages Deposit Your Completed Plasmids Find the...

...translocations. It also provides researchers with many different ways to control gene expression. Get the full ...Joanne Kamens explains what career coaches do and offers up some career coach suggestions. 8. A Match Made...

... investigate the susceptibility of cells from different organisms to SARS-CoV-2 (Chu et al., 2020). An... of the scientific research infrastructure. References Chu H, Chan JF-W, Yuen TT-T, Shuai H, Yuan S,...

...time for exercise. Many studios and gyms are now offering online classes and participating in these is a... Jennifer Tsang...

...light-induced cell ablation with MG-2I-dL5** in two different experimental settings: HEK293 cell culture and...using photosensitizers in the comments below!   References 1. He, J., Wang, Y., Missinato, M. A., Onuoha...

...have made it easier to track translation in two different ways: by monitoring the first round of translation...various cell types and physiological states. References 1. Ingolia, Nicholas T., et al. “Genome-wide ...

...Facility Isotype IgG2a Differences:  Distinct variable domains that recognize different epitopes on GFP Monoclonal...

...restriction enzymes since the instructions use NEB buffers. Nonetheless, if you are setting up a double digest... provides a list of directions including which buffers to use and that indicates if a sequential digest...

...inactive state. The dynamic range, defined as the difference between the lowest and highest level of “output...various scientific or engineering applications.   References Refactoring and optimization of light-switchable...

...of prominent members of the bioscience and tech transfer communities who help guide and promote Addgene... of how miRNAs regulate their targets and what confers specificity in the miRNA regulatory process. This...

... proteins whose excitation and emission spectra differ sufficiently for them to be imaged in distinct ...fluorescent channels on the microscope. With the proliferation of fluorescent proteins in recent years, there...

...and SV40 promoters, are always active and thus referred to as constitutive promoters. Others are only ...Experimental System Because transcription machinery differs between cell types or organisms, promoters are ...

... web of opportunities that the classroom cannot offer. Furthermore, companies across most industries tend...helps you find internships and scholarships in different subject areas!    2. Resume and cover letter...

...SARS-CoV-2 proteins for downstream experiments and offer flexibility in tag, cleavage, codon usage, and expression...Visit our COVID-19 Plasmids and Resources Page! References Fukushi S, Watanabe R, Taguchi F (2008) Pseudotyped...

...culture). This dilution step represents the major difference between PACE and PANCE: for PANCE the phage progeny...much more efficiently and in more contexts. References Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran...

...the system and the flexibility to address many different types of research questions. Are you ready to ...experiments? Find EvolvR plasmids at Addgene! References Gaj, Thomas, and Pablo Perez-Pinera. "The continuously...

...Listen to the C. elegans RNAi podcast segment RNA interference (RNAi) is extensively used in C. elegans research...endogenous RNAi pathways. These mutations however can interfere with other genetic pathways making it difficult...

... and precisely identifies plasmid features that differ by only a few nucleotides, such as the fluorescent...static image file (PNG, see above) for your own reference or to paste directly into your notebook. This ...

...peptide. By combining three different localization peptides with four different fluorescent proteins, and...

... the cutting. While sonication is usually the preferred method, enzymatic digestion can be appropriate...protocols when embarking on your ChIP journey!  References and Resources More resources on Addgene’s blog...

...method’s flexibility allows it to be used with different types of PCR-generated inserts. By harnessing ...updated by Emily P. Bentley in September 2024.  References and Resources Further reading Harnessing homologous...

...high and your immunogenicity low! References and Resources References Robinson HL. Retroviruses and cancer...

...al. applied TRIM-away to nine proteins in ten ddifferent cell types, including difficult-to-manipulate...that TRIM21 overexpression did not affect cell proliferation. These cells also did not contain changes in...

... invasive to use optogenetics in the brain.  References Bedbrook CN, Yang KK, Robinson JE, Mackey ED,...Read more about optogenetics Learn about the differences between chemogenetics and optogenetics Resources...

...function of the protein and pdDronpa are preserved. References 1. Zhou, Xin X., Linlin Z. Fan, Pengpeng Li, ...28232577. Figures 2-4 were modified from this reference. 2. Regot, Sergi, Jacob J. Hughey, Bryce T. Bajar...

...This post was originally written by Jennifer Tsang and updated by Rachel Leeson. Have you ever wondered...period. For ease of calculations*, let’s split the difference and put scientific inflation at… roughly 34.9%...

...us develop these resources further. Feel free to offer your comments and suggestions, and even to submit...submit a guest blog entry of your own! References 1. Bernard, P. “Positive Selection of Recombinant DNA ...

... plasmid libraries containing combinations of different gRNAs in each plasmid. Each gRNA combination can...systems. He can be contacted at [email protected].   References 1. Cheng, Allen A., Huiming Ding, and Timothy...

...and industry jobs. Since there are a variety of different career paths that don’t all need another degree...-op. It has been so insightful to learn about different career paths, successes and failures, values, ...

...virtual bookshelf. Pro tip The “cited by” and references sections of a PubMed entry are great places to...This library can also be synced with a Mendeley reference manager account. Pro tip Sign up for weekly email...

... to address the unique needs of students from different departments across the university and alterations...public policy. Follow David on Twitter @driglar.   References: 1. Alberts, Bruce, et al. "Rescuing US biomedical...

...academic labs. Being unable to publish or attend conferences makes it very challenging to compete in today...pressure to publish and more freedom to attend conferences, but after receiving her degree she was faced...

...species, like S. aureus and N. meningitidis require different PAM sites than SpCas9, in this case, NNGRRT and...is working for you in the comments section! References Hu, Johnny H., et al. "Evolved Cas9 variants ...

...treatment (Stephens et al., 2019). If sufficient gene transfer and knock-in is achieved, producer cells could...and CRISPR resources on Addgene's CRISPR page. References Beatty MS, Curiel DT (2012) Adenovirus Strategies...

...explaining CRISPR technology to 4th graders, check out Jennifer Doudna’s new book A Crack in Creation. Making... the target DNA strand, and then activate two different endonuclease active sites. Fortunately, there ...

...against improper, random recombination into a different locus. Correctly recombined cells will not contain...should increase. New genome engineering tools also offer hope for the creation of new knockout animal models...

... the process is similar to academia. The only difference is that your paper will be reviewed by an internal...LinkedIn connections and other contacts to get that referral and find out more about the company’s program....

...comes down to the researcher’s familiarity with different systems.  Using Cas9-gRNA RNPs in your experiments...include genome engineering and microbiology.     References 1. Chen, Sean, et al. "Highly efficient mouse...

...expertise with our district middle school was a very different experience since, unlike elementary schools, middle...such a valuable extension to our curriculum and offered engaging examples of how understanding genetics...

...truth, people are incredibly varied in how they prefer to consume information. Personally, I would much...outcome to the limitations and potential of your preferred platform before you start can save a lot of trouble...

...human genome-wide CRISPRko (knock out), CRISPRi (interference), and CRISRPa (activation) libraries that do...conditions such as model systems and primary cells. References 1. Doench, John G., et al. "Rational design of...

...mixed population, represented here by cells of different colors, specifically targets bacteria with the...ground in the fight against bacterial superbugs. References 1. Citorik, Robert J., Mimee, Mark, and Timothy...

...Joanne’s back yard this year, though she generously offered. Instead we picniced outside of our new office ...Netflix series. The thing that has made all the difference here at Addgene is the emphasis placed on team...