We narrowed to 881 results for: NSI

1. Plasmids 101: Codon usage bias

   Type

   Blog Post

   ...2008), but there are still many more features to consider when designing a full gene. A non-exhaustive list...

2. Using Video to Share Your Science: We Share Science

   Type

   Blog Post

   ...Evolution of Scientific Impact Data Freedom: The Expansion of Data Sharing in Research Publications Resources...

3. Rett Syndrome: A History of Research and Therapeutic Outlooks

   Type

   Blog Post

   ...fine-tuning dosage so that enough wild-type MECP2 is made inside the cells, but this is exciting progress in tackling...

4. To Codon Optimize or Not: That is the Question

   Type

   Blog Post

   ...Molecular Biology Reference Read about genetic code expansion Browse our plasmid collection page  ...

5. Overcoming the AAV Size Limitation for CRISPR Delivery

   Type

   Blog Post

   ...streptavidin and next-generation sequencing). Using this sensitive method, they found that SaCas9 did not display...

6. The PAM Requirement and Expanding CRISPR Beyond SpCas9

   Type

   Blog Post

   ...Wolfe SA, Sontheimer EJ (2018) NmeCas9 is an intrinsically high-fidelity genome-editing platform. Genome...

7. Plasmids 101: Broad Host Range Plasmids

   Type

   Blog Post

   ...host range recombinant vectors Replicons, which consist of a plasmid’s ori and all of its control elements...

8. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

   Type

   Blog Post

   ...plant and animal systems. Importantly, they allow transient delivery, enabling transgene-free genome editing...

9. Transferable Skills Guide: Time Management

   Type

   Blog Post

   .... For instance, even if I had planned my day extensively, my lack of desire to go sit in the tissue culture...

10. Harnessing Bacterial Toxins for Allelic Exchange

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    Blog Post

    ...unmarked mutants, but it frequently requires considerable optimization to ensure strong counter-selection...

11. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type

    Blog Post

    ... and model systems. We have used CRISPR/Cas9 extensively for the purpose of making sequence-specific changes...

12. Thoughts on the Future of Research 2015 Boston Symposium

    Type

    Blog Post

    ...different universities are trying to compile more comprehensive demographic information about the postdoc work...

13. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type

    Blog Post

    ...Commons 4.0 license. The research groups responsible for Easi-CRISPR have also recently developed ...

14. CRISPR 101: Epigenetics and Editing the Epigenome

    Type

    Blog Post

    ...cells. For example, KRAB induced silencing was transient and quickly reversed in culture. However, DNMT3A-induced...

15. Technique: Probe Phage Genomes for Host Binding Proteins

    Type

    Blog Post

    ...transformants for appropriately sized inserts. We consider a library worth using if at least 40% of colonies...

16. Plasmids 101: Restriction Cloning

    Type

    Blog Post

    ...stains):  Stain Pre or Post Run? Visualization Sensitivity (ng DNA) SYBR Safe Pre and Post Blue or UV ...

17. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type

    Blog Post

    ...possible a significant qualitative and quantitative expansion in the 2015-2016 academic year. This year, the...

18. A History of Genome Engineering in Popular Culture

    Type

    Blog Post

    ... scientific community and  remind us to always consider the ethics and broader implications of our research...

19. DNA Quantification

    Type

    Protocol

    ...such as TE). You will get much more accurate and consistent readings from DNA in a buffered solution. Reference...

20. Protocol - How to Design Primers

    Type

    Protocol

    ...for broader pairing capabilities. Taking into consideration the information above, primers should generally...

21. CRISPR 101: Multiplex Expression of gRNAs

    Type

    Blog Post

    ...be expressed simultaneously. Vectors for both transient expression and Agrobacterium-mediated transformation...

22. Protocol - How to Perform Sequence Analysis

    Type

    Protocol

    ... experiments. There are a number of factors to consider when selecting sequencing primers, including: ...

23. Protocol - How to Perform a Diagnostic Digest

    Type

    Protocol

    ... to test individual clones before use of more expensive forms of plasmid verification, such as DNA sequencing...

24. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

    Type

    Protocol

    ...Watch the Video! How to Design a Primer Key considerations when designing primers Watch the Video! Sequence...

25. Lab Safety for Biosafety Levels One and Two

    Type

    Protocol

    ...anyone else associated with Addgene, can be held responsible for your use of the information contained in ...

26. Protocol - Bacterial Transformation

    Type

    Protocol

    ...The lowest efficiency cells (usually the least expensive) are fine for transforming plasmid DNA for the...

27. Lentivirus Production

    Type

    Protocol

    ...batch of 1 mg/mL PEI and for each cell line. Considerations Before You Start The health of the packaging...

28. Protocol - How to Run an Agarose Gel

    Type

    Protocol

    ...high percentage of glycerol that increases the density of your DNA sample causing it settle to the bottom...

29. Plasmid Cloning by PCR (with Protocols)

    Type

    Protocol

    ...Cloning The basic PCR primers for molecular cloning consist of: Leader Sequence: Extra base pairs on the 5'...

30. Antibody Validation Using the Indirect ELISA Method

    Type

    Protocol

    ...support reproducibility in science. General Considerations The ELISA can be completed in a single day ...

31. Protocol - pLKO.1 – TRC Cloning Vector

    Type

    Protocol

    ...suitable for handling HIV-derivative viruses. For transient knockdown of protein expression, you may transfect...