...and how do they come off and on in a cellular environment? If these are pressing issues in your laboratory...majority of FRET acceptors are delivered to the wrong address in a different city, you can still develop...list Acceptor labeling: Sometimes it's okay to be wrong In contrast to FRET donors, absolute FRET acceptor...

...pMD2.G VSV-G envelope expressing plasmid Didier Trono pVSV-G Expresses VSV-G envelope protein for...are used in the lab? Check out our “Rabies and Neuronal Tracing” blog post! You can also browse tools ...

...lentivirus envelope plasmid pMD2.G from Didier Trono’s lab! Lentivirus envelope vector pMD2.G This plasmid...protocols and information, be sure to check out the Trono Lab website. If you have plasmids you’d like to ...

...around the world!) that have kept Addgene at the forefront of life sciences research for the past decade....not just for a better day at the office but for stronger science as well. This blog is our way of continuing...

...spatial, cellular, metabolic, and cytokine microenvironment that exists in vivo. Over the past five years...cell type, in more complex and physiological microenvironments. These screens have primarily been performed...

...developing a strong graduate student community and the need for belonging in the scientific environment 3:09 ...

...spatially with Cre-recombination. Read the paper in Frontiers in Molecular Neuroscience Find the AAV-based...can be used in diverse bacteria from various environments. It has the advantage of stable genomic integration...

...they have a graduate degree in science. I find it ironic that we do all of our training as scientists (5...jobs (that is what networking is about), but be upfront about interest in a job. Ask openly to be connected...

...activity and geotactic behavior (Biron et al., 2005).   References Biron, D. G., et al. "Behavioural manipulation...

...liberated to adorn the walls of universities and the front pages of newspapers. Science is now being adapted...while - a tool to expand perception, which is ironically what science is about. For me it is essential...

...important to this function. With the welcoming environment on my team and at Addgene, I felt comfortable...seemed. The transition into a traditional work environment was aided by the efforts being put in by Addgene...

...results in the degeneration of spinal cord motor neurons. This disease is estimated to affect anywhere from...for use in the clinic and thereby restore motor neuron function in spinal muscular atrophy patients. Noushin...

...increasingly accessing websites other than through the ‘front door,’ the homepage is still important. It needs...easily. In the redesign, the new search bar is now front and center, with a clear call to action - Search...

... tobacco, Arabidopsis, and corn. For both β-glucuronidase (GUS) and GFP reporters, as well as multiple...Transgenesis with GreenGate Plasmids Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More Plasmids...

...test recombination efficiency. When expressed in neuronal cells, each line showed broad recombinase-dependent...hamster ovary (CHO) cells, as well as in cortical neurons in layer 2/3 of the mouse brain. Rubin’s work shows...

...vehemently about the need for change.  Dr. Bourne strongly believes this is an amazing time of promise for... Novartis industry postdoctoral program.  His strongest comments were about the astounding lack of reproducibility...

...interested in meeting movie stars, but when I met the astronaut Jim Lovell I was speechless (the club of people...students in their labs and the importance of the strong, cross-disciplinary collaboration they shared to...

...that there are many ways your experiments can go wrong. Whether it be a bad fridge freezing your samples...editing and later looking for songs and sounds to synchronize with the images thereby making the videos more...

...Structure and Function. Frontiers in Microbiology, 7. https://www.frontiersin.org/articles/10.3389/fmicb...

...Enhancer traps do not need to be integrated within an intron or exon of a gene, and thus they do not disrupt...splice acceptors. They require insertion into an intron of a gene, where they can be spliced into the gene...