We narrowed to 682 results for: RON
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TypeBlog Post...co-workers who want to make our organization as environmentally friendly as possible. We worked with Andrew... unless we manage to make Addgene the most environmentally friendly organization on the planet, in which...easier. Trust Ultimately, your team could have a strong sense of purpose, an excellent leader, and perfectly...
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A History of Genome Engineering in Popular Culture
TypeBlog Post...carrier of genetic information and led to the coronation of DNA as the molecule of life. This was further...genetic engineering experiment which goes horribly wrong. The plot of the movie involves The Rock and his....111.131433 Doudna JA, Charpentier E (2014) The new frontier of genome engineering with CRISPR-Cas9. Science... -
Special Delivery: Fluorophore Targeting for FRET Studies
TypeBlog Post...and how do they come off and on in a cellular environment? If these are pressing issues in your laboratory...majority of FRET acceptors are delivered to the wrong address in a different city, you can still develop...list Acceptor labeling: Sometimes it's okay to be wrong In contrast to FRET donors, absolute FRET acceptor... -
Export Control Regulations Lifted for Plasmids Containing Rabies and VSV-G Genes
TypeBlog Post...pMD2.G VSV-G envelope expressing plasmid Didier Trono pVSV-G Expresses VSV-G envelope protein for...are used in the lab? Check out our “Rabies and Neuronal Tracing” blog post! You can also browse tools ... -
Top Requested Plasmid of 2017 - pMD2.G
TypeBlog Post...lentivirus envelope plasmid pMD2.G from Didier Trono’s lab! Lentivirus envelope vector pMD2.G This plasmid...protocols and information, be sure to check out the Trono Lab website. If you have plasmids you’d like to ... -
Addgene’s New Way to Help You Share Science
TypeBlog Post...around the world!) that have kept Addgene at the forefront of life sciences research for the past decade....not just for a better day at the office but for stronger science as well. This blog is our way of continuing... -
Performing In Vivo CRISPR Screens Using the FITS Approach
TypeBlog Post...spatial, cellular, metabolic, and cytokine microenvironment that exists in vivo. Over the past five years...cell type, in more complex and physiological microenvironments. These screens have primarily been performed... -
Grad School Advice Part 2: Building Community
TypeBlog Post...developing a strong graduate student community and the need for belonging in the scientific environment 3:09 ... -
What's New in CRISPR - Spring 2019
TypeBlog Post...spatially with Cre-recombination. Read the paper in Frontiers in Molecular Neuroscience Find the AAV-based...can be used in diverse bacteria from various environments. It has the advantage of stable genomic integration... -
Scientist Networking: What is an Informational Interview?
TypeBlog Post...they have a graduate degree in science. I find it ironic that we do all of our training as scientists (5...jobs (that is what networking is about), but be upfront about interest in a job. Ask openly to be connected... -
Six Spooky Science Stories and Halloween at Addgene
TypeBlog Post...activity and geotactic behavior (Biron et al., 2005). References Biron, D. G., et al. "Behavioural manipulation... -
Science as Art
TypeBlog Post...liberated to adorn the walls of universities and the front pages of newspapers. Science is now being adapted...while - a tool to expand perception, which is ironically what science is about. For me it is essential... -
Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot
TypeBlog Post...increasingly accessing websites other than through the ‘front door,’ the homepage is still important. It needs...easily. In the redesign, the new search bar is now front and center, with a clear call to action - Search... -
Working 9-5: an Addgene Co-op
TypeBlog Post...important to this function. With the welcoming environment on my team and at Addgene, I felt comfortable...seemed. The transition into a traditional work environment was aided by the efforts being put in by Addgene... -
Supporting Rare Disease Research with BeHeard 2017
TypeBlog Post...results in the degeneration of spinal cord motor neurons. This disease is estimated to affect anywhere from...for use in the clinic and thereby restore motor neuron function in spinal muscular atrophy patients. Noushin... -
pSiM24: Simplifying Plant Genetic Engineering
TypeBlog Post... tobacco, Arabidopsis, and corn. For both β-glucuronidase (GUS) and GFP reporters, as well as multiple...Transgenesis with GreenGate Plasmids Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More Plasmids... -
Cre-ating New Methods for Site-specific Recombination in Drosophila
TypeBlog Post...test recombination efficiency. When expressed in neuronal cells, each line showed broad recombinase-dependent...hamster ovary (CHO) cells, as well as in cortical neurons in layer 2/3 of the mouse brain. Rubin’s work shows... -
Future of Research Conference - Remarkable Opening Session
TypeBlog Post...vehemently about the need for change. Dr. Bourne strongly believes this is an amazing time of promise for... Novartis industry postdoctoral program. His strongest comments were about the astounding lack of reproducibility... -
Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner
TypeBlog Post...interested in meeting movie stars, but when I met the astronaut Jim Lovell I was speechless (the club of people...students in their labs and the importance of the strong, cross-disciplinary collaboration they shared to... -
CUT&RUN: An Improved Method for Studying Protein-DNA Interactions
TypeBlog Post...of input material, cells or tissue, to produce a strong enough signal over background noise. This is not...protocol, the most significant being the usage of a strong detergent to permeabilize cells rather than to ...