We narrowed to 353 results for: SAM activators
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TypeBlog Post...the latest fluorescent proteins, I maintain an interactive graph and table of the best fluorescent proteins...be imaged together, you will need to consider the same factors as when choosing an individual fluorescent...
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GCE4All: Making Genetic Code Expansion Accessible
TypeBlog Post...modification, or add in fluorescent or chemically reactive side chains, all of which can help determine structure...naturally, rarely are the optimization parameters the same for all three components, meaning that the final... -
Celebrating 15 Years of Scientific Sharing
TypeBlog Post...community is loving it. Almost 10,000 viral vector samples were distributed in 2018 and we are poised for ...Work with a positive, supportive culture, we are attractive to the local employee pool and we get a lot of... -
Popular Retroviral Vectors and Their Uses in Scientific Research
TypeBlog Post...both lentiviruses and gamma-retroviruses use the same gene products for packaging, however, the isoforms...(Plasmid #44246) Expression of a catalytically inactive (dead), human codon-optimized Cas9 under the control... -
Twenty Years of Sharing: Expanding Accessibility
TypeBlog Post...that available and accessible weren’t always the same thing, shaped both Addgene’s founding and our growth...design in our digital content. We have implemented proactive measures to find potential barriers that may not... -
Using Video to Share Your Science: We Share Science
TypeBlog Post...their disciplines. Included in the article is an interactive chart where you can see the yearly interdisciplinarity...amount of interdisciplinary research while at the same time increasing interdisciplinary citations in their... -
Adeno-associated Viruses (AAVs) for Genome Editing
TypeBlog Post...require modifying multiple genes simultaneously in the same cell, are more appropriate for a CRISPR-based approach... mechanisms and consequences of cohesin gene inactivation in human cancer. References 1. Russell, ... -
Thoughts on the Future of Research 2015 Boston Symposium
TypeBlog Post...Saturday Hack Day projects sought to make an interactive game that highlights just this point. As part...underrepresented groups under their mentorship with the same critical eye they place on others within their labs... -
Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons
TypeBlog Post...three loxP variants, but the principles remain the same. The first fluorescent protein in the construct,...proteins and confirmed that there was no cross-reactivity, opening the door for immunostaining analysis... -
Adenovirus Guide
TypeGuide...rAdV vectors are highly immunogenic because they activate both innate and adaptive immune responses in the... elements into the genome, often altering gene activity. Oncolytic Referring to the destruction of cancer...in cis refers to genetic elements located in the same plasmid as the gene of interest. in trans In the... -
Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation
TypeBlog Post...differentiation.” Specific cell media, supplements, bioactive small molecules, and growth factors are used to...human induced pluripotent stem cells from urine samples. Nat Protoc, 2012. 7(12): p. 2080-9. PubMed PMID... -
Sequencing Primers
TypeGuide...AATACCACTACAATGGAT (BD Biosciences) 3' end of Gal4 activation domain, forward primer GFP-F GGTCCTTCTTGAGTTTGTAAC...CCCTTGAACCTCCTCGTTCGACC (MSCV) Murine stem cell virus, same as MSCV, forward primer pMRB101-F AAGATGCAGGCAGCTGAGTT...GATTTAATCTGTATCAGG (Invitrogen) 3' of MCS in pTrcHis vector, same as pBAD-R, reverse primer Puro-F GCAACCTCCCCTTCTACGAGC... -
Molecular Biology Reference
TypeGuide...luciferase or GFP) that offers a read-out of the activity of the genetic element. For instance, a promoter...daughter cells. These daughter cells contain the same genetic information as the parental cell, and are...