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Showing: 341 - 360 of 382 results

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10 Steps to a Perfect Science Talk

Type

Blog Post

...listeners to be thinking “What happens next?” Describe how you pushed to a solution or overcame obstacles...
How to Negotiate a Successful Lab Start Up Budget

Type

Blog Post

...importantly, it’ll help you define salary costs. 4. Ascribe a cost structure associated to these tools, skills...
Technique: Probe Phage Genomes for Host Binding Proteins

Type

Blog Post

...identify possible gene function. In this post, I will describe a useful and cost-effective method that our lab...
CRISPR 101: Epigenetics and Editing the Epigenome

Type

Blog Post

...histone modifications. Like the TALE-LSD1 system described above, dCas9-LSD1 inactivates targeted enhancers...
Viral Vectors 101: Optogenetic Tools

Type

Blog Post

...In 2005, Boyden et al. described the first light-activated tool for controlling neuronal activity, channelrhodopsin...
Antibodies 101: Multiplex Immunofluorescence

Type

Blog Post

...between the targets. Or, it might be sufficient to describe or classify the labeled structures visually; for...
Viral Vectors 101: AAV Serotypes and Tissue Tropism

Type

Blog Post

... capsid of another. Formally, pseudotypes are described with two numbers separated by a slash: the first...
Important Considerations When Using AAVs

Type

Blog Post

... but many of these tips apply to all tissues. Subscribe to Viral Vector Blog Posts from Addgene 1. Location...
Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

Type

Blog Post

...BAC DNA can be modified with any of the below described approaches. Bacterial strain with integrated...
Plasmids 101: Using Transposons in the Lab

Type

Blog Post

... on a reverse transcriptase enzyme to reverse transcribe back into DNA before being inserted into new ...
An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

Type

Blog Post

...available through Addgene. In 2019, Farboud et al. described another similar co-conversion marker (repair of...
FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

Type

Blog Post

...the opsin into the correct orientation to be transcribed (Figure 2) (Sharma and Zhu, 2014). How does FLEx...
Custom CRISPR Screens & the Green Listed Software

Type

Blog Post

...sequences, and more. Output_Compact: A text file that describes which genes were included, and how many gRNAs ...
Cancer and the Immune System: Deciphering the Relationship

Type

Blog Post

... tested and will help healthcare professional prescribe and identify potential beneficiaries. Collaborative...
Plasmids 101: Modular Cloning Applications and Kits

Type

Blog Post

...are designed for flexibility. The Fragmid kit described above works in both mammalian systems and Drosophila...
Special Delivery: Fluorophore Targeting for FRET Studies

Type

Blog Post

...it all together The FRET donors and acceptors described above can be paired together to measure a wide...
A Needle in a Base-Stack: Cas9 Structural Biology

Type

Blog Post

...Fact! Even though the Cas9:RNA complex is well described in the closed conformation, recent electron microscopy...
Optogenetics + CRISPR, Using Light to Control Genome Editing

Type

Blog Post

...transcriptional activators p65 and VP64 which in turn transcribes N-Cas9. The N- and C-Cas9 fragments then dimerize...
Immunology Research Plasmids and Resources

Type

Collection

...immune response. Over 60 interleukins have been described. NK Cell Signaling Natural killer (NK) cells can...immune response. Over 60 interleukins have been described. Symbol Name Synonyms EBI3 Epstein-Barr Virus ...
CRISPR 101: Cytosine and Adenine Base Editors

Type

Blog Post

...named ABEmax. In 2020, two papers were published describing additional ABEs evolved from ABE7.10 that have...

Showing: 341 - 360 of 382 results

Results per page:

10 Steps to a Perfect Science Talk

How to Negotiate a Successful Lab Start Up Budget

Technique: Probe Phage Genomes for Host Binding Proteins

CRISPR 101: Epigenetics and Editing the Epigenome

Viral Vectors 101: Optogenetic Tools

Antibodies 101: Multiplex Immunofluorescence

Viral Vectors 101: AAV Serotypes and Tissue Tropism

Important Considerations When Using AAVs

Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

Plasmids 101: Using Transposons in the Lab

An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

Custom CRISPR Screens & the Green Listed Software

Cancer and the Immune System: Deciphering the Relationship

Plasmids 101: Modular Cloning Applications and Kits

Special Delivery: Fluorophore Targeting for FRET Studies

A Needle in a Base-Stack: Cas9 Structural Biology

Optogenetics + CRISPR, Using Light to Control Genome Editing

Immunology Research Plasmids and Resources

CRISPR 101: Cytosine and Adenine Base Editors

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