We narrowed to 405 results for: expression vector

...cleavage, Bac-to-Bac Expression System, AmpR 26100 pFHMSP-LIC-C N/A Expression vector for secreted proteins...TEV cleavage, AmpR 26095 pFHMSP-LIC-N N/A Expression vector for secreted proteins with N-terminal hexahistidine...SGC Toronto SGC Oxford SGC Stockholm Bacterial Expression Plasmids (empty backbones/controls) ID Plasmid...Thrombin cleavage, Bac-to-Bac Baculovirus Expresion vector 26117 pNIC-CH EF199843 Hexahistidine tag, AmpR ...hexahistidine tag and TEV cleavage, Bac-to-Bac Expression System, AmpR Related Resources SGC: www.thesgc.org...

...luciferase reporter vectors for sensitive gene expression analysis and fusion vectors containing tags such...luciferase reporter vectors for sensitive gene expression analysis and protein fusion vectors for detecting ... from an enzymatic reaction to measure subtle expression changes with high sensitivity. These assays are...enables highly sensitive detection at endogenous expression levels. NanoLuc fusions are useful for both intracellular...want minimal disruption of endogenous protein expression and interactions, such as exploring viral biology...HaloTag technology is optimized for various protein expression systems including E. coli , mammalian cells, ...interest is fused to either LgBiT or SmBiT and expressed in live cells. When the two proteins interact,...

...-minBglobin-iCre-4X2C-WPRE3-BGHpA AAV vector for Cre expression in forebrain GABAergic interneurons. For...version of DLX enhancer). AAV vector for strong & rapid SYFP2 expression in forebrain GABAergic interneurons...sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells The Allen...Fan 221463-AAV1 pAAV_BiPVe4_eGFP AAV construct expressing eGFP driven by chandelier cell-targeting enhancer...Wilson 221463-PHPeB pAAV_BiPVe4_eGFP AAV construct expressing eGFP driven by chandelier cell-targeting enhancer...-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN5140) Expression of CoChR-EGFP in striatal cholinergic neurons..._3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN4825) Expression of iCre(R297T) in striatal medium spiny neurons...

...These proteins can be expressed in specific neurons, often utilizing viral expression systems, to activate...Insert Vector Type Promoter PI Return to top Additional Resources Optogenetics Guide Viral Vector Packaging...and kinetic properties as well as improve the expression and membrane targeting in host cells. Other microbial...fluorescent protein to the opsin in order to track its expression and localization. Many other types of proteins...optogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter Eligible for Viral Packaging PI Return...

...Assembly method Nuclease Expression Vectors - Cloning vectors for expressing zinc finger FokI nuclease...Find reagents for engineering and expressing zinc finger proteins deposited by Zinc Finger Consortium... Addgene various reagents for engineering and expressing zinc finger proteins for distribution to academic...

...enzymes into an expression vector of their choice and then transform into E. coli for expression and purification...their own enzymes by cloning them into an expression vector of their choice and transforming into E. coli... and protocols for expression in the Open Bioeconomy Lab's crowd-sourced expression guides (Link opens...Return to top Additional Resources Bacterial Expression Vectors Synthetic Biology Plasmids MoClo Guide Drew...and codon-optimized the Open Enzymes parts for expression in Escherichia coli . Researchers can use the...purification ( pET28a-LIC or other pET vectors are recommended). The selection marker for all plasmids in ... are also compatible with the E. coli Protein Expression Toolkit (Link opens in a new window) — all Open...

... with viral vector manufacturing facilities, such as the University of Pennsylvania Vector Core. Transfections...Pennsylvania Vector Core. In general, titering is performed by the facility that produced the viral vector lot....Transducibility Some viral vectors are tested in vitro and in vivo for gene expression and/or function. These...Empty vector particles can be identified after negative staining and appear darker than full vector particles...Electron micrograph of AAV vector preparation shows that the vast majority of the vectors consist of full particles... Viral Vector Packaging Service Viral Production Viral Production...to determine which facility produced your viral vector lot. At Addgene, AAV particles are titered by droplet...

... Viral Vector Packaging Service AAV Serotype Testing AAV Viral Vector Packaging Service: ...available from Addgene's viral service. Control EGFP vectors in various serotypes for serotype testing: AAV1...addition to the standard 100 µL sample size, the vectors listed below are also available as 20 µL. The ...does not have the suffix (for example, AAV1). AAV Vectors for Serotype Testing pAAV-CAG-GFP (Plasmid #37825... (deposited by Edward Boyden ) and direct GFP expression from the CAG promoter. For information about ...50465 (deposited by Bryan Roth ) and direct EGFP expression from the human synpasin promoter. For information... eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know...

...MK, Elfinger M, Hartl D, Rudolph C. Transgene expression of transfected supercoiled plasmid DNA concatemers... JA, Carnes AE, Hodgson CP. Plasmid DNA vaccine vector design: impact on efficacy, safety and upstream...

...shRNAs are often delivered using viral vectors. Lentiviral vectors are a preferred method for long-term ...constitutive expression as well as those that allow for conditional (Cre-lox) or inducible (Tet) expression are...Plasmid Vector Type PI Publication Additional Resources Addgene Resources pLKO.1 cloning vector protocol...long-term gene silencing. Once the viral vector is taken up by a host, the viral genome is transcribed, producing.... shRNA Plasmids Browse our selection of empty vectors for cloning in shRNAs. Most plasmids are in a lentiviral...Lentiviral packaging plasmids Web Resources Trono Lab Lentivectors Toolbox Optimized shRNA design in pSico with...

...TALEN Expression Vectors for REAL, REAL-Fast and FLASH TALE Transcriptional Activator Expression Vectors...sites and to simplify the construction of TALEN expression plasmids using REAL, REAL-Fast or FLASH assembly...

... Plasmid Collections Bacterial Expression Systems Bacterial Expression Systems Protein Purification Protein...bacterial expression systems. Read below and browse our curated collection of bacterial expression plasmids...Addgene Blog E. Coli Strains for Protein Expression Protein Expression Epitope Tags Protein Tags Return to ...to top Controlled Expression It isn’t always advantageous to constitutively express your gene of interest...turn on expression only at a specific time. This collection contains plasmids whose expression levels ...Addgene Controlled Expression Resources Check out our Tetracycline (Tet) Inducible Expression Collection for...Find bacterial expression plasmids that can be used for protein purification, protein visualization, ...

...for many molecular biology protocols, including vector-insert joining for recombinant plasmid construction..."Cloning, nucleotide sequence, and engineered expression of Thermus thermophilus DNA ligase, a homolog...Barany, Francis, and David H. Gelfand. "Cloning, overexpression and nucleotide sequence of a thermostable DNA...: PMC146030. 26. Nishida, Hirokazu, et al. "Overexpression, purification and crystallization of an archaeal...

... top Empty Prime Editing gRNA Vectors A selection of empty gRNA vectors suitable for prime editing are...below. Use the search bar to find a gRNA vector based on expression system, promoter, the type of gRNA (e.g...Cas9. ID Plasmid Expression System Promoter Guide RNA Type Cloning Enzyme Co-expressed Cas9 Selection PI...

...mammalian expression systems. To find emtpy gRNA plasmids, visit Addgene's Empty gRNA Expression Vectors page... CRISPR Mammalian Expression CRISPR Plasmids: Mammalian Expression Activate Base Edit Cut Epigenetics...

...Microbiology Plant Expression Stem Cells Synthetic Biology Tet Inducible Expression Worm Expression Kits Kits ... Fluorescent Proteins Protein Expression Synthetic Biology Viral Vectors Communities Find a scientific... Topics Antibody Plasmid Collection Bacterial Expression Systems Empty Backbones Genetic Code Expansion...

...Transl Med. 2013) Express human DNMT1 with a Myc tag. (Li et al. J Biol Chem. 2006) Express human ATM with...Science Collection . Express EGFP-tagged wt and mt tau . (Hoover et al. Neuron. 2010) Express EGFP-tagged wt...Neurodegeneration Plasmid Collection A collection of plasmids expressing wild type or mutant ORFs, gene fragments, and...to neurodegenerative disorders. Find plasmids expressing HTT, C9ORF72, LRRK2, Synuclein, and more. Study...Flag tag. (Bennett et al. Heliyon. 2020) Use VAP expression plasmids to study ER contact with other organelles...Convert iPSCs to neurons with inducible lentiviral expression constructs: pLVX-UbC-rtTA-Ngn2:2A:EGFP (Schafer...Ascl1 (Herdy et al. Elife. 2019) Use plasmids expressing SOX10, OLIG2, and NKX6.2 transcription factors...

...a scientist to control signaling within cells expressing these engineered receptors. Chemogenetic receptors... the specific cell type where the receptor is expressed, these receptors can be used to artificially activate...chemogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter Eligible for Viral Packaging PI Additional... Additional Resources Chemogenetics Guide Viral Vector Packaging Service In-Stock AAV: Chemogenetics Fluorescent...

...visualize genomic loci. gRNAs Empty gRNA Vectors Select an empty vector for your experiment based on a variety... fused to an activator peptide activates gene expression. Interfere dCas9 alone, or fused to a repressor...epigenetic modifier alters chromatin structure/gene expression. RNA Targeting RNA Targeting Type VI CRISPR systems...

...cloning vector Retroviral vectors: neomycin (pSIR-neo) , GFP (pSIR-GFP) , DsRed (pSIR-DsRed-Express2) , human... sgRNA dual expression: pAC2 , pAC152 , pAC153 , pAC154 ; pmax dCas9 activator expression: pAC91 , pAC92... pooled library amplification 1 vector system: lentiCRISPR v2 2 vector system: lentiCas9-Blast and lentiGuide-Puro...Addgene Church gRNA design and cloning gRNA cloning vector PDF, 118 KB Church gRNA design and cloning for ...human pluripotent stem cells pCas9_GFP ; gRNA empty vector Protocol at StemBook O'Connor-Giles Fly: gRNA and...PDF, 330 KB Parrott NEBuilder Assembly of CRISPR vectors using ssDNA oligos p201G Cas9 ; p201B Cas9 ; p201H...design and cloning All-in-one plasmid containing expression cassette for NmCas9 and BsmBI site for cloning...