We narrowed to 413 results for: his

1. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

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   ...mutate in the genome) and an out-of-frame H2B (histone)-GFP reporter into the genome of mammalian cells...

2. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

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   ...and TEV), and C-terminal tags (StrepII, 3xFLAG, 6xHIS, MBP, Myc). These constructs include proteins like...

3. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

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   ...Plasmids Check out our CRISPR Guide Read our CRISPR History  ...

4. Improved Plasmid Maps Powered by SnapGene

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   ...promoters, origins, tags, and certain ORFs. Their sophisticated detection algorithm easily and precisely identifies...

5. Antibodies 101: ChIP

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   ...antibody on crosslinked proteins. In the case of histone modifying proteins, which bind quite strongly to...

6. Hot Plasmids - February 2022

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   ...separately. The proteins, which are purified via histidine tags, are optimized for SDS-PAGE gels and can ...

7. Antibodies 101: Isotypes

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   ...antibody-based applications including flow cytometry, immunohistochemistry, immunocytochemistry, ELISAs, and Western...

8. Viral Vectors 101: Biosensors

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   ...of the biosensors used in research are very sophisticated, but at their core they can all be boiled down...

9. How to Make Friends and Meet People at a Scientific Conference

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   ...Conference Dinner - by Julio Peironcely on the Thesis Whisperer "Not Networking - Building Relationships for Success...

10. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

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    ...coordinate emotions, thoughts, and behavior. Historically, scientists study the electrical activity in...

11. Reproducibility for Everyone: Lessons from an Open Science Collaboration

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    ... Schwessinger for helpful conversations on the history of the group, lessons learned, and to Sonali Roy...

12. Antibodies 101: Selecting the Right Antibody

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    ...be variations within an application. Take immunohistochemistry (IHC), for instance. While an antibody may...

13. Antibodies 101: Conjugation

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    ...typically used in microscopy-based assays like immunohistochemistry, though they are also a common choice for...

14. CRISPR 101: Cas9 vs. The Other Cas(s)

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    ...to detect variants such as single nucleotide polymorphisms. Fast facts – PAM requirement: none. Best ...

15. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

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    ...ccdB accomplish this? Let’s start with a brief history of the gene and how molecular biologists have harnessed...

16. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

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    ...repurpose popular GFP reporter lines for more sophisticated experimental manipulations, saving the time ...

17. Career Coaching for Scientists: Why and Where Do I Find One?

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    ...has an outstanding blog and, while her PhD is in History, I think she would be a good match as a coach for...

18. Working with Nuclear Receptors

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    ...other members of the complex, such as p300 and CBP histone acetyltransferases or CARM1 methyltransferase. ...

19. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

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    ...-CBP In this method, dCas9 is fused to CBP, an histone acetyltransferase domain capable of rearranging...

20. Engaging with science and society at pgEd

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    ...sticky ethical dilemmas to dark episodes in the history of eugenics and research misconduct. Past ethical...