...pipette, it might make sense to earn a master’s instead of a PhD. Deciding to pursue a master’s is a big...

...determine the optimal localization of the Rluc8 insertion within the Gα. Despite exhaustive refinement, ...

...research interests. “So for me, I researched G-proteins at the time. This program takes what you know ...

... Eric J. Perkins...

...Availability of superiors for consultation, advice and brainstorming. Employees must understand the part they play...

...resources where possible.  At the lunch table, for instance, we have a plastic container problem…reusable ...

...start her own research group at Boyce Tompson Institute.  References Baldwin, Melinda. "Credibility, peer...

...factors for generating iPSCs Learn how fluorescent proteins help researchers study single cell dynamics in...

...22154564. 6. Stoneham, Charlotte A., Michael Hollinshead, and Amin Hajitou. "Clathrin-mediated endocytosis...

...so why and when should you opt to make a virus instead of just zapping your cells? That really depends...

...particles containing complementary polynucleotide chains. J Virol 9(2): 394-396. Lecomte E, Tournaire B,...

...Christopher Harvey’s lab. These plasmids localize opsins to the soma to avoid off target photostimulation...

...resources on the Addgene blog Learn about other Cas proteins Read all of our CRISPR blog posts Read about GeoCas9...

...and we’ve been using "plasmid" ever since! From napkins to notebooks Although discovered in the early 1950s...

...network Attend informational interviews to gain insight into the daily routine of the potential job Join...

...journals as those that did make deposits to Addgene.  Insomuch as different journals represent different research...

...available and then decide on a course of action. Instead of rushing in, you take steps to make it more likely...

...can also request the specific serotypes you need instead of receiving a kit with all the serotypes, since...

...in non-human primates, where fusion fluorescent proteins can affect DREADD trafficking and localization...

... consensus to the next meeting of experts, and instead convene the necessary groups for the necessary ...

...topics and interests. Offer help when you can, instead of just asking for favors.  Don’t focus on networking...

...human cells via delivery of purified Cas9 ribonucleoproteins." Genome research 24.6 (2014): 1012-1019....

... the end product would likely be poor quality. Instead, we pull together teams of individuals who enjoy...

...Plasmid Collections Fluorescent Proteins Activity Regulation Fluorescent Proteins: Activity Regulation More ...Biosensors Optogenetics Background Fluorescent proteins such as GFP have long been used for labeling, ... methods are emerging to use these fluorescent proteins to modulate biological activity. Early tools allow...

...- Davidson Allen Institute for Brain Science AAV Enhancer Collection Allen Institute for Cell Science ...Collections Depositor Collections Addgene's repository contains 158,806 plasmids, which includes plasmid collections...Reagents for improved expression of recombinant phosphoproteins - Rinehart FreeGenes Project Open Enzyme Collection...

...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter PI...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert Promoter Selectable...

...recruit specific RNA-binding proteins (RBPs) tagged with fluorescent proteins to visualize targeted genomic...requires recruitment of many copies of labeled proteins to the given region. For example, chromosome-specific...throughout the chromosome. Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Do you...

...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter Selectable...subsequently isolated by affinity purification against the epitope tag. Compared to conventional methods...Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Do you...

...DREADDs, PSAMs Optogenetics Channelrhodopsins, Halorhodopsins, Archaerhodopsins Recombinases Cre, Flp, Dre...Sensors, Voltage Reporters Controls Fluorescent Proteins, Spagehetti Monster, Brainbow Molecular Tools ...

...joining (NHEJ), a mechanism which frequently causes insertions or deletions (indels) in the DNA. Indels often...repair a DSB using homology-directed repair (HDR) instead of NHEJ. In most experimental systems, HDR occurs...much lower efficiency than NHEJ. ID Plasmid Gene/Insert Selectable Marker PI Publication Libraries Toxoplasma...knockout gRNA pooled library designed with 10 guides against 8,158 predicted protein-coding genes. Do you have...

...vectors, the target site sequence can be added by inserting an oligonucleotide linker into BsaI digested vector...for generating gRNAs 106.5 KB flyCRISPR website contains additional information CRISPR Optimal Target Finder...other genomic sequence with an attP docking site. Contains the visible marker 3xP3-DsRed. (Also known as ...generating dsDNA donors for homology-directed repair. Contains the visible marker 3xP3-DsRed....

...regulate transcription. Red flame plasmid pDawn contains the blue-light sensing protein YFI. When light...

... using your single color controls (Figure 1A), instead of using your actual samples. They may also be ...

...AAVrg while other pathways such as those in the brainstem were not. Others have noted a lack of transduction...

...careers Plasmids CRISPR Viral vectors Fluorescent proteins  ...

...which often focuses more attention on the results instead of the methods. Unreported flexibility and questionable...

...They all found the support of the group to be intrinsic to their success. Their stories made it clear ...

...joining (NHEJ), a mechanism which frequently causes insertions or deletions (indels) in the DNA. Indels often...repair a DSB using homology-directed repair (HDR) instead of NHEJ. In most experimental systems, HDR occurs...much lower efficiency than NHEJ. ID Plasmid Gene/Insert Promoter PI Publication Activate Catalytically ...activator to your specific locus. ID Plasmid Gene/Insert Promoter PI Publication Empty gRNA Expression Vectors...

... L-Myc, Lin28, OCT3/4, and shRNA against p53 in different gene/insert combinations A more efficient method...regenerative medicine. iPSCs, much like their ES cell cousins, have the hallmarks of pluripotent cells--they ...Ottosson Stem Cell Research Plasmids This table contains a general list of plasmids that have been used...name, species, gene name, and more. Plasmid Gene/Insert Vector Type PI Publication Do you have suggestions...

...oligonucleotide (ssODN) template supplied by Ma et al., but instead from the mother’s wild type allele (Figure 1). ...

...up in both science journals like Nature and in mainstream media like The Atlantic. We’ve been in documentaries...

... with very little text on the slides and notes instead of written speeches. When you’re doing all that...

...addition to the 20 bp guide sequence, the crRNA also contains a ~20 bp repeat region on its 3’ end. This repeat...

...scientists can receive recognition for their efforts, inspire future research, and increase their impact. Open...

... Eric J. Perkins...

...Novartis Oncology.  Bhang did her PhD work at Johns Hopkins and came to Novartis for her post-doc in January...

...seriously. As I make my rounds speaking at research institutions and at scientific conferences, I see that few...

...for targeting enzymes and other useful protein domains to a specific DNA sequence ( Figure 1A ). Scientists...consisting of 12-31 repeats can be assembled and inserted into a variety of backbones in just a few steps...Toolbox", from Dr. Feng Zhang’s group at the Broad Institute, will be available from Addgene before the end...Pubmed PMID: 21822241 . TAL effectors: customizable proteins for DNA targeting Bogdanove AJ, Voytas DF. Science...

...since these processes are mediated by other viral proteins), but the newly released RABV cannot infect any...

...certain cell types.  Botond Roska’s lab at the Institute of Molecular and Clinical Ophthalmology Basel ...