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We narrowed to 398 results for: AGA

Showing: 361 - 380 of 398 results

  11. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...with pLKO.1 sequencing primer (5’ CAA GGC TGT TAG AGA GAT AAT TGG A 3’). TIP: You may need to adjust the...Qiagen #28704 Low melting point agarose Sigma #A9414 Luria Broth Agar (LB agar) American Bioanalytical: #AB01200...all capital letters. I.2. Recipes Luria Broth Agar (LB agar) + antibiotic Per 40 grams of powder from American...5g yeast extract 10g sodium chloride 15g agar Prepare LB agar solution by dissolving 40g of LB powder ...Consortium has built a library of shRNAs directed against 15,000 human and 15,000 mouse genes. Addgene is...hours. Run digested DNA on 0.8% low melting point agarose gel until you can distinctly see 2 bands, one 7kb..., following manufacturer’s protocol. Plate on LB agar plates containing 100 μg/mL ampicillin or carbenicillin...

  12. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...is taken up on one side of the cell and released again at another side of the cell after intracellular ...

  15. CRISPR Library Amplification

    Type
    Protocol
    ...mL of LB Agar per bioassay plate. 14 mL Vented Falcon Tubes (BD Biosciences, 352059) 3X LB Agar + Antibiotic...minutes). Prewarm 3X LB Agar + Antibiotic plates at 37 °C. Prewarm 8X LB Agar + Antibiotic Bioassay plates... by the agar. This usually takes 1-2 minutes. Critical Be careful not to rip or shred the agar. Do so ...single time and visualize that digestion on an agarose gel ( see restriction digest protocol here ). Lentiviral...containing only the elements required for bacterial propagation (origin of replication and antibiotic selection...20 mL SOC recovery media (Lucigen, 80026-1) 8X LB Agar + Antibiotic 245 mm bioassay plates (Molecular Devices... Preparation Prepare, sterilize, and pour all LB Agar + Antibiotic plates. Prewarm 12 mL recovery media...

  16. Protocol - How to Streak a Plate

    Type
    Protocol
    ...Isolating Bacteria on an LB Agar Plate You may also like... Making LB Agar Plates Bacterial Transformation...Reagents LB agar plate (with appropriate antibiotic) Bacterial stab Procedure Obtain an LB agar plate with...bacteria on an LB agar plate to obtain single colonies. Protocols... Protocols Streaking Bacteria on LB Agar Plate Streaking...stock. Using a single colony from a freshly streaked agar plate to inoculate a bacterial culture for DNA purification...single bacterial colony by streaking it onto an LB agar plate. Last Update: Feb. 28, 2017 Protocol Video...touch the surface of the plate, do NOT dig into the agar. Another very popular technique is to draw in discontinuous...

  17. Video Library

    Type
    Protocol
    ...Page Making LB Agar Plates Create plates to culture bacteria in the lab Pouring LB Agar Plates Protocol...Protocol Over-Agar Antibiotic Plating Create plates to culture bacteria in the lab Over-Agar Antibiotic ...and purification. Agarose Gel Electrophoresis Protocol Purifying DNA from an Agarose Gel Gel purification...on Plates Isolate single bacterial colonies on an agar plate Streaking Bacteria on Plates Protocol Diagnostic...Titration by qPCR Using SYBR Green Technology Protocol Agarose Gel Electrophoresis Gel electrophoresis is the ...electrophoresis, you can cut DNA bands out of the agarose gel and purify the DNA samples. This is a commonly...restriction enzyme-based cloning. Purifying DNA from an Agarose Gel Protocol How-To Videos (Link opens in a new...

  18. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

    Type
    Protocol
    ...Making LB Agar Plates Create plates to culture bacteria in the lab Watch the Video! Over-Agar Antibiotic...restriction enzymes Agarose Gel Electrophoresis Separate DNA by size on an agarose gel Watch the Video...Bacteria Isolate single bacterial colonies on an agar plate Watch the Video! Inoculating a Liquid Bacterial...and FAQ Watch the Video! Purifying DNA from an Agarose gel Isolate specific DNA bands cut from a gel Watch...the Indirect ELISA Method Run an indirect ELISA against a purified antigen to demonstrate the expected ...

  19. Protocol - Bacterial Transformation

    Type
    Protocol
    ...there isn't too much liquid media on the agar plates. If the agar plate doesn't dry adequately before the...Microcentrifuge tubes Sterile spreading device Reagents LB agar plate (with appropriate antibiotic) LB or SOC media...and thaw on ice (approximately 20-30 mins). Remove agar plates (containing the appropriate antibiotic ) ...to grow once plated on the antibiotic containing agar plate. This step is not critical for Ampicillin ...some or all of the transformation onto a 10 cm LB agar plate containing the appropriate antibiotic. Pro-Tips... went wrong? Check that you are plating on an LB Agar plate containing the correct antibiotic. The resistance...

  20. Protocol - How to Inoculate a Bacterial Culture

    Type
    Protocol
    ... on your LB agar plates are not fresh, you should streak your bacteria onto a new LB agar plate before...like... Streaking and Isolating Bacteria on an LB Agar Plate Creating Bacterial Glycerol Stocks for Long-term...commonly used to culture bacteria in the lab. LB agar plates are frequently used to isolate individual...2 O to 400 mL Note: If your lab has pre-mixed LB agar powder, use the suggested amount, instead of the...or toothpick, select a single colony from your LB agar plate . Drop the tip or toothpick into the liquid...
Showing: 361 - 380 of 398 results