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We narrowed to 464 results for: LARGE

Showing: 361 - 380 of 464 results

  2. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...useful for intracellular staining where binding of large immune complexes may be hindered. Indirect staining...information about the relative size of the cell; larger cells produce more FSC. Light that scatters sideways...

  4. Pouring LB Agar Plates

    Type
    Protocol
    ... you will be screening a large number of colonies, we recommend using larger plates. Many labs use 100...

  8. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...transfected with low efficacy. But since there can be large differences between endogenous expression and transfected... This can be particularly useful when designing larger multicolour panels, where different manufacturers...

  9. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...distributing widely throughout the organism to target large or diffuse biological structures. Of primary interest...transduction in Old World monkeys AAV.CAP-Mac is largely neurotropic in infant rhesus macaques and green...AAVs to transduce the same cell, can accommodate larger, modular genetic payloads. Since most systemic ...

  10. AAV Production in HEK293 Cells

    Type
    Protocol
    ...efficient means to scale-up without having to handle a large number of T-175 flasks. Workflow Timeline Day 0:... (PES) filter system, Nalgene, 565-0010 (or for larger volumes 1000 mL 0.45 µm Rapid-Flow PES Filtration...

  11. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...redshifted-emission spectrum compared to that of CyOFP1, a large stokes shift, long fluorescence lifetime, and a ...imaging. These variants were developed as part of a larger strategy to engineer improved red fluorescent proteins...

  12. CRISPR Guide

    Type
    Guide
    ... Browse Plasmids: Prime Edit Large Scale Edits CRISPR can generate large-scale genetic changes, including...CRISPR Guide Overview CRISPR Basics Small Edits Large Edits Genome-Wide Screening Cas9 Fusion Tools Cas9...knockout or gene edit; or using Cas9 to generate large genomic deletions or inversions with two gRNA sites...plasmid or linear dsDNA templates work better for large edits or insertions. When designing the donor template...increase HDR efficiency. In most applications, a large portion of the Cas9-induced DSBs will still be repaired...Transposon systems like piggyBac or Tn7 allow for large scale edits by integrating segments of DNA flanked..., developed by the Feng Zhang lab ) combine the large-scale capacity of transposases with the precise ...

  13. Guide to Using Pooled Libraries

    Type
    Guide
    ...designed to cover only a subset of genes, or very large (e.g. the Toronto KnockOut (TKO) library from the... of cells as plasmids in the TKO library. Using large numbers of cells minimizes the effects of random... CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens New Tool for Lineage Tracing...

  14. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...activates the necessary cellular machinery to produce large amounts of rAAV vectors. The E1 gene is not included...distributing widely throughout the organism to target large or diffused biological structures. Specifically,...visualize connections between neurons within the brain. Large-scale examples of mapping the brain through neuronal... capacity Another strategy to deliver cargo too large to fit within the rAAV capsid, such as Cas9-derived...which has shown higher efficiency in delivering large genes makes use of split inteins, proteins that ...be expressed. This method allows for delivery of larger transgenes, but expression is still much less efficient...

  15. All Antibodies

    Type
    Collection
    ...produce antibodies are available as part of Addgene’s larger Antibody Plasmid Collection , which also includes...

  16. Resolute Plasmid Collection

    Type
    Collection
    ...Feedback Solute carrier proteins (SLCs) are the largest family of transporters in the human genome with...

  17. Serotype Testing AAV

    Type
    Collection
    ...performance of various serotypes before conducting larger scale experiments. For more information, see our...

  18. Lentiviral Vector Guide

    Type
    Guide
    ...First-generation First-generation lentiviral plasmids are largely defunct due to their lack of safety improvements...on different plasmids, the viral genome remains largely intact. First-generation plasmids include (Figure...full-length virus. The potential for oncogenesis is largely based on whether or not the specific insert contained...identifies alterations in chromatin architecture through large-scale mapping of lentiviral integration sites. Nature...

  19. Adenovirus Guide

    Type
    Guide
    ...vectors, rAdV vectors can provide a large transgene packaging capacity. Large protein-coding genes and complex... and third-generation vectors. This capacity is large enough to carry the Cas9 gene and a gRNA expression...shorter period of time or when needing to package larger cargo. CRISPR-Cas9 Delivery As compared to other...backbone plasmid that lacks E1, E3, and E4. Useful for larger inserts. pAdTrack Class of shuttle/transfer vectors...

  20. Plan Your Experiment

    Type
    Guide
    ...alleles will not contain the desired edit, as a large percentage of DSBs are still repaired by NHEJ. How... and gel electrophoresis (for HDR or NHEJ): For large deletions or insertions, the region of interest ...
Showing: 361 - 380 of 464 results