We narrowed to 497 results for: RAP

1. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

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   Blog Post

   ...19563763. PubMed Central PMCID: PMC2753793. 3. V. A. Rudrapatna, et al. Dev Dyn. 241. 107–118 (2012). PubMed ...

2. Multiplex Genome Editing with CRISPR-Cpf1

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   Blog Post

   ...plasmid plus a Cpf1 expression vector.  See the graph in figure 2 for a comparison of editing frequency...

3. A Quick Guide to a Career in Software Product Management

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   Blog Post

   ...what the day to day looks like. Check out the infographic for an outline of my day or find the text version...

4. New and Upcoming Viral Vectors - May 2020

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   ...and holds promise in the development of future therapies. Our popular plasmid encoding a Cre dependent ...

5. Sharing is Caring: But How to Distribute Open Hardware?

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   ...of commercially available tools and components. Rapid prototyping techniques such as 3D printing, on-demand...

6. Antibodies 101: Monoclonal Antibodies

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   ...hybridomas  Learn about monoclonal antibodies for immunotherapy Resource on Addgene.org Check out Addgene’...

7. Hot Plasmids - May 2022

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   ...likely to afford the ability to deliver more therapeutics to the CNS, and with reduced systemic side effects...

8. AAV Q&A with Tim Miles

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   ...discussed in our recent review.      And that's a wrap on our AAV webinar! Special thanks to our panel ...

9. Hot Plasmids - February 2022

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   Blog Post

   ...possibilities in both cell engineering and gene therapy.  Xiaoshu X, et al. Molecular Cell. 2021. https...

10. Sweating the Small Stuff: Details in the Lab

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    Blog Post

    ...small, avoidable mistake. Or worse, when you're trapped in a pattern of small errors that significantly...

11. Antibodies 101: Buffers, Storage, and Conjugates

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    Blog Post

    ... to be stored either in a dark, opaque vial or wrapped in aluminum foil to prevent ambient light from ...

12. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

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    Blog Post

    ...pandemic in which scientists race to find vaccines, therapeutics, and other treatments to stem the spread of ...

13. Developing a cold tolerant E. coli using specialized chaperones

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    Blog Post

    ...tires give a better grip in the cold, but wear out rapidly in warm weather. Psychrophilic enzymes face very...

14. CasPEDIA: A Functional Classification of Cas Enzymes

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    ...to relevant resources The homepage displays a graphic describing CasIDs and relevant properties of Cas...

15. Communicating Your Science With Help From ComSciCon

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    Blog Post

    ...science communication comes with practice. You can bootstrap your science communication skills with training...

16. Why and how to start studying chromatin (with Addgene's help)

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    Blog Post

    ...Gerlich DW Cell. 2017 Aug 24;170(5):956-972.e23 Rapid and phosphoinositol-dependent binding of the SWI...

17. Plasmids 101: Inducible Promoters

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    Blog Post

    ...inducer, like Tet systems, may be activated very rapidly. In contrast, promoter systems that require transcription...

18. Top Requested Lentivirus and AAV of 2016

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    Blog Post

    ...Cusher DS, Maratos-Flier E, Roth BL, Lowell BB. Rapid, reversible activation of AgRP neurons drives feeding...

19. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

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    Blog Post

    ...three strategies described in this post in the graphic below - including some simple comparisons. The...

20. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

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    Blog Post

    ... use in various cell types and in live mice. Therapeutic uses in humans are also on the table. “In the...