We narrowed to 682 results for: RON
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TypeBlog Post...CRISPR is a sleek acronym for a real mouthful of a phrase: Clustered Regularly Interspaced Short Palindromic...parts! If you’re still a bit confused by CRISPR acronyms, this post is for you. We’ll cover the common ...
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Year of the Camelid: Antibody Style
TypeBlog Post...Antibodies: Historical Perspective and Future Outlook. Front Immunol 8:1589. https://doi.org/10.3389/fimmu.2017.01589... Ashley Waldron... -
Antibodies 101: Fc Effector Functions
TypeBlog Post...Structure and Function. Frontiers in Microbiology, 7. https://www.frontiersin.org/articles/10.3389/fmicb... -
Plasmids 101: Gene, enhancer, and promoter trapping
TypeBlog Post...Enhancer traps do not need to be integrated within an intron or exon of a gene, and thus they do not disrupt...splice acceptors. They require insertion into an intron of a gene, where they can be spliced into the gene... -
Bricking Science: Portraying Scientific Reality Through LEGO
TypeBlog Post...that there are many ways your experiments can go wrong. Whether it be a bad fridge freezing your samples...editing and later looking for songs and sounds to synchronize with the images thereby making the videos more... -
The Stingy Scientist: How the Baby Gel Box Was Born
TypeBlog Post...It is probably good news for our environment that more people are paying attention to living a “green...reagents and buffers. However, I had very environmentally conscious friends who had me thinking about... -
Summer SciComm Series: Master of SciComm
TypeBlog Post...interests and areas of expertise. Perhaps the strongest aspect of science communication, then, is its ...allowed to percolate within a new intellectual environment. Over time, this interest came to incorporate... -
Addgene Begins Distribution of Recombinant Antibodies
TypeBlog Post...of Experimental Methods. Neuron 90(3):434-6. https://doi.org/10.1016/j.neuron.2016.04.030 Bradbury ... -
Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring
TypeBlog Post...novel organisms and enzymes from these extreme environments. Gearing up for sample collection at the hot...Navigating challenges: collecting and processing environmental samples Since the high temperatures and unstable... -
HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides
TypeBlog Post...the widely used HA epitope in diverse cellular environments. They called this probe the HA frankenbody (...is effective in sensitive cells such as living neurons, and can be fused to other fluorescent proteins... -
Identifying Sequence Elements with SnapGene's Feature Database
TypeBlog Post...possible option, we place the most important controls front and center, and make specialized controls available...plasmids were inconsistent and sometimes clearly wrong. In an effort to be rigorous, we dug into the original... -
New and Upcoming Viral Vectors - December 2019
TypeBlog Post...with ever more efficient tools for light mediated neuronal control and we are expanding our viral service...photostimulation of axons and dendrites on neighboring neurons. We have also packaged hChR2(H134R) plasmids from... -
Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?
TypeBlog Post...gene-editing protein - a DNA-cleaving Argonaute from Natronobacterium gregoryi, or NgAgo for short. Addgene has ... Han. “DNA-guided genome editing using the Natronobacterium gregoryi Argonaute.” Nat Biotechnol. Epub ... -
Even more elegant: Single injection CRISPR/Cas9 in C. elegans
TypeBlog Post...elegans, they inserted the SEC into a synthetic intron between a fluorescent protein (FP) and a 3xFLAG...resulting loxP scar is located in this synthetic intron, and only the fluorescent protein and 3xFLAG-tag... -
Using CRISPR/Cas9 to Edit Disease Out of the Genome
TypeBlog Post...disease mutation in basal forebrain cholinergic neurons derived from iPSCs. From Rohn et al. 2018; see ... Limitations, and Implications for the Future.” Front. Oncol. https://doi.org/10.3389/fonc.2020.01387 ... -
Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools
TypeBlog Post...concentration of ApoB-LPs has been shown to have a strong correlation with cardiovascular disease and indirect... (GEVIs) change fluorescence in real time when neurons or muscle cells transmit electrical information... -
Visualizing Genomic Loci with CRISPR-Sirius
TypeBlog Post...CRISPRainbow. Both tools were targeted to the FBN3 intronic repeat which contains 22 copies of the gRNA target...distances of loci pairs from intergenic regions, intronic regions, and a pericentromeric region. All pairs... -
Improving Transduction Efficiency with Exosome AAVs
TypeBlog Post...cells (as demonstrated in different cellular environments such as retina or glioblastoma). They also found.... Exo-AAV9 was successfully shown to transduce neurons in the brain after systemic (i.v.) delivery in ... -
Pushing the Limits of DNA Assembly
TypeBlog Post...Assembly method from New England Biolabs® has minimal upfront requirements and allows you to expediently join...-executed cloning endeavor, NEBuilder requires upfront planning. This cloning methodology requires that... -
Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS
TypeBlog Post...genes and wanted to express them within a specific neuronal compartment, you could express them individually...Z. Marka, I. Bartos, S. Marka, J. C. Kagan, Environmental Stress Causes Lethal Neuro-Trauma during Asymptomatic...