Skip to main content
Addgene
Showing: 361 - 380 of 671 results

  2. Build Your CRISPR Vocabulary

    Type
    Blog Post
    ...CRISPR is a sleek acronym for a real mouthful of a phrase: Clustered Regularly Interspaced Short Palindromic...parts! If you’re still a bit confused by CRISPR acronyms, this post is for you. We’ll cover the common ...

  3. Year of the Camelid: Antibody Style

    Type
    Blog Post
    ...Antibodies: Historical Perspective and Future Outlook. Front Immunol 8:1589. https://doi.org/10.3389/fimmu.2017.01589... Ashley Waldron...

  4. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    ...It is probably good news for our environment that more people are paying attention to living a “green...reagents and buffers.  However, I had very environmentally conscious friends who had me thinking about...

  5. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    ...gene-editing protein - a DNA-cleaving Argonaute from Natronobacterium gregoryi, or NgAgo for short. Addgene has ... Han. “DNA-guided genome editing using the Natronobacterium gregoryi Argonaute.” Nat Biotechnol. Epub ...

  7. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    ...with ever more efficient tools for light mediated neuronal control and we are expanding our viral service...photostimulation of axons and dendrites on neighboring neurons. We have also packaged hChR2(H134R) plasmids from...

  10. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    ...interests and areas of expertise. Perhaps the strongest aspect of science communication, then, is its ...allowed to percolate within a new intellectual environment. Over time, this interest came to incorporate...

  13. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    ...number, but it was attained without any of the error-prone repair that normally accompanies Cas9 cutting. We... holds some kind of fitness advantage but error-prone repair of the gene would be disastrous (i.e. if ...

  14. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    ...disease mutation in basal forebrain cholinergic neurons derived from iPSCs. From Rohn et al. 2018; see ... Limitations, and Implications for the Future.” Front. Oncol. https://doi.org/10.3389/fonc.2020.01387 ...

  15. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ...high GC content and hairpin structures, ITRs are prone to replication errors and disruptive mutations and...improvements unrelated to capsid protein modification. Front Med, 10, 1106085. https://doi.org/10.3389/fmed.2023.1106085...

  16. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...generating knock-in mutations, and NHEJ is an error-prone pathway frequently introducing frameshift knockout...Emerging Model Organisms in Fundamental Research. Front. Microbiol. 11-2020, 2021. DOI: 10.3389/fmicb.2020.607028...

  17. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...Assembly method from New England Biolabs® has minimal upfront requirements and allows you to expediently join...-executed cloning endeavor, NEBuilder requires upfront planning. This cloning methodology requires that...

  19. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    ...shipped), where they would be exposed to a closed environment containing CO2 (from the dry ice). After 48 hours...PMID: 23538862. 2. Potter M, Lins B, Mietzsch M, Heilbronn R, Van Vliet K, Chipman P, Agbandje-McKenna M,...

  20. Addgene Depositors Get More Citations

    Type
    Blog Post
    ...10.5%  3.2  534.1 3 2010 3 Applied and Environmental Microbiology  1.6%  1.2% 0.75   2.3 4 2010... 2010 167  Applied and Environmental Microbiology  1.6% 1.2%  0.75   125.3 In figure 2, you can...
Showing: 361 - 380 of 671 results