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We narrowed to 676 results for: RON

Showing: 361 - 380 of 676 results
  1. New and Upcoming Viral Vectors - December 2019

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    Blog Post
    ...with ever more efficient tools for light mediated neuronal control and we are expanding our viral service...photostimulation of axons and dendrites on neighboring neurons. We have also packaged hChR2(H134R) plasmids from...
  2. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    ...interests and areas of expertise. Perhaps the strongest aspect of science communication, then, is its ...allowed to percolate within a new intellectual environment. Over time, this interest came to incorporate...
  3. A Tour of Addgene's Most Popular Pooled Libraries

    Type
    Blog Post
    ...library: the Cultivarium MACKEREL Library, whose acronym means Modular, NGS-trACKable ExpRession Element...for binding properties. For example, several coronavirus libraries have been used to systematically explore...
  4. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    ...gene-editing protein - a DNA-cleaving Argonaute from Natronobacterium gregoryi, or NgAgo for short. Addgene has ... Han. “DNA-guided genome editing using the Natronobacterium gregoryi Argonaute.” Nat Biotechnol. Epub ...
  5. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ...for successful transduction. In another example, neuronal gene expression by an anterograde AAV was shown...input-defined neural pathways for defense behaviors." Neuron 93.1 (2017): 33-47. PubMed PMID: 27989459. PubMed...
  6. Pushing the Limits of DNA Assembly

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    Blog Post
    ...Assembly method from New England Biolabs® has minimal upfront requirements and allows you to expediently join...-executed cloning endeavor, NEBuilder requires upfront planning. This cloning methodology requires that...
  7. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    ...shipped), where they would be exposed to a closed environment containing CO2 (from the dry ice). After 48 hours...PMID: 23538862. 2. Potter M, Lins B, Mietzsch M, Heilbronn R, Van Vliet K, Chipman P, Agbandje-McKenna M,...
  8. Addgene Depositors Get More Citations

    Type
    Blog Post
    ...10.5%  3.2  534.1 3 2010 3 Applied and Environmental Microbiology  1.6%  1.2% 0.75   2.3 4 2010... 2010 167  Applied and Environmental Microbiology  1.6% 1.2%  0.75   125.3 In figure 2, you can...
  9. Visualizing Genomic Loci with CRISPR-Sirius

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    Blog Post
    ...CRISPRainbow. Both tools were targeted to the FBN3 intronic repeat which contains 22 copies of the gRNA target...distances of loci pairs from intergenic regions, intronic regions, and a pericentromeric region. All pairs...
  10. Improving Transduction Efficiency with Exosome AAVs

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    Blog Post
    ...cells (as demonstrated in different cellular environments such as retina or glioblastoma). They also found.... Exo-AAV9 was successfully shown to transduce neurons in the brain after systemic (i.v.) delivery in ...
  11. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    ...disease mutation in basal forebrain cholinergic neurons derived from iPSCs. From Rohn et al. 2018; see ... Limitations, and Implications for the Future.” Front. Oncol. https://doi.org/10.3389/fonc.2020.01387 ...
  12. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...generating knock-in mutations, and NHEJ is an error-prone pathway frequently introducing frameshift knockout...Emerging Model Organisms in Fundamental Research. Front. Microbiol. 11-2020, 2021. DOI: 10.3389/fmicb.2020.607028...
  13. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    ...number, but it was attained without any of the error-prone repair that normally accompanies Cas9 cutting. We... holds some kind of fitness advantage but error-prone repair of the gene would be disastrous (i.e. if ...
Showing: 361 - 380 of 676 results