We narrowed to 494 results for: control
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TypeBlog Post...difficult to accurately call a file. Version control your commonly used scripts: If you edit a general...
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CRISPR 101: Non-Homologous End Joining
TypeBlog Post...Video] Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens ... -
Lentivirus Plasmids
TypeCollection...pLVTHM 2nd EF-1a-driven GFP and shRNA under the control of a tet-responsive H1 promoter Trono 11651 pLVUT-tTR-KRAB... -
Viral Vectors 101: Systemic Capsids
TypeBlog Post...neurons. (b) Genetic cargo can be customized to control transgene expression. Gene regulatory elements .../off genetic switch can be used for expression control. (c) Viral delivery methods include focused ultrasound...to be aware of before starting an experiment. Controlling expression As systemic capsids are… well, systemic...tissues and cell types. If desired, spatiotemporal control of transgene expression or activity can be achieved... -
Viral Vectors 101: Calcium Sensors
TypeBlog Post...fluorescent state only by simultaneous (1) user-controlled illumination with violet light and (2) exposure... -
Hot Plasmids and Viral Preps - March 2021
TypeBlog Post...single Cas9-VPR in Saccharomyces cerevisiae. To control Cas9 activity and influence DNA repair pathways... -
Choosing the B(right)est Fluorescent Protein: Photostability
TypeBlog Post...substrate. These in vitro methods allow for a well-controlled environment, but they do not mimic the natural... -
Rosella: A Fluorescent pH-Biosensor for Studying Autophagy
TypeBlog Post...this is indicative of protein degradation). To control for protein degradation due to other pathways, ... -
CRISPRainbow and Genome Visualization
TypeBlog Post...of the Pitfalls When Using Fluorescent Proteins Control Protein Activity with GFP Resources on Asddgene.org... -
Plasmids 101: CcdB - The Toxic Key to Efficient Cloning
TypeBlog Post...PubMed PMID: 1324324. 6. Tam, JE. and Kline, BC. “Control of the ccd operon in plasmid F.” J Bacteriol. 1989... -
The Breast Cancer Microenvironment: A Tumor’s Backstage Team
TypeBlog Post...decrease the expression of TAK 1, an enzyme that controls apoptosis and regulates transcription. Without... cannot undergo apoptosis, thus multiplying uncontrollably and building up tumors. “Friendly” microenvironment... -
Popular Retroviral Vectors and Their Uses in Scientific Research
TypeBlog Post...inactive (dead), human codon-optimized Cas9 under the control of Murine Stem Cell retrovirus promoter for mammalian... -
Plasmids 101: FLEx Vectors
TypeBlog Post...transduced reporter gene. Generating a FLEx switch to control expression of an optogenetic reporter would ensure... -
Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening
TypeBlog Post... Luke A., et al. "Genome-scale CRISPR-mediated control of gene repression and activation." Cell 159.3 ... -
Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing
TypeBlog Post... etc)? Did they perform all of the appropriate controls? Refer back to the methods section for finer points... -
Science Guides
TypeGuide...selectively control and investigate cell signaling pathways. Receptors are remotely controlled via small...small molecules, which allows for specific control of activity. Read More CRISPR Class 2 C lustered R egularly...research utilize light to detect, measure, and control molecular signals and cells in order to understand... -
Chemogenetics Guide
TypeGuide... However, neuronal control through PSAMs and LMOs is direct, while neuronal control of DREADDs is indirect...endogenous signaling, therefore allowing precise control over the cell population they are targeted to. ...used in the same organism to allow bidirectional control of neuronal activity. Table 1. Descriptions of ...chemogenetic receptors that confer more direct control of neurons through manipulation of ion channels...expression of AAV-delivered constructs can be controlled with cell-type specific promoters. Table 4 lists...unwanted cell types. Generating a FLEx switch to control expression of a chemogenetic reporter ensures that...AI, Armbruster BN, Pei Y, et al. (2009). Remote control of neuronal activity in transgenic mice expressing... -
Neurodegeneration Research Collection
TypeCollection...neurons in the substantia nigra, one of the movement control centers in the brain. The cause of this neuron ...Symptoms include changes in behavior and emotion, uncontrolled movements, memory deficits, as well as difficulty... -
Making CRISPR Plasmids Using Fragmid
TypeBlog Post... in the hands of the Doench lab. Additionally, controls available in the system are validated and were... -
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation
TypeBlog Post...Ming G-L, King JR, Song H, Sweatt JD (2013) TET1 Controls CNS 5-Methylcytosine Hydroxylation, Active DNA...