We narrowed to 495 results for: control

...neurons. (b) Genetic cargo can be customized to control transgene expression. Gene regulatory elements .../off genetic switch can be used for expression control. (c) Viral delivery methods include focused ultrasound...to be aware of before starting an experiment. Controlling expression As systemic capsids are… well, systemic...tissues and cell types. If desired, spatiotemporal control of transgene expression or activity can be achieved...

...single Cas9-VPR in Saccharomyces cerevisiae. To control Cas9 activity and influence DNA repair pathways...

...fluorescent state only by simultaneous (1) user-controlled illumination with violet light and (2) exposure...

...inactive (dead), human codon-optimized Cas9 under the control of Murine Stem Cell retrovirus promoter for mammalian...

...decrease the expression of TAK 1, an enzyme that controls apoptosis and regulates transcription. Without... cannot undergo apoptosis, thus multiplying uncontrollably and building up tumors. “Friendly” microenvironment...

...substrate. These in vitro methods allow for a well-controlled environment, but they do not mimic the natural...

...this is indicative of protein degradation). To control for protein degradation due to other pathways, ...

...of the Pitfalls When Using Fluorescent Proteins Control Protein Activity with GFP Resources on Asddgene.org...

...PubMed PMID: 1324324. 6. Tam, JE. and Kline, BC. “Control of the ccd operon in plasmid F.” J Bacteriol. 1989...

...transduced reporter gene. Generating a FLEx switch to control expression of an optogenetic reporter would ensure...

... Luke A., et al. "Genome-scale CRISPR-mediated control of gene repression and activation." Cell 159.3 ...

... etc)? Did they perform all of the appropriate controls? Refer back to the methods section for finer points...

...selectively control and investigate cell signaling pathways. Receptors are remotely controlled via small...small molecules, which allows for specific control of activity. Read More CRISPR Class 2 C lustered R egularly...research utilize light to detect, measure, and control molecular signals and cells in order to understand...

... However, neuronal control through PSAMs and LMOs is direct, while neuronal control of DREADDs is indirect...endogenous signaling, therefore allowing precise control over the cell population they are targeted to. ...used in the same organism to allow bidirectional control of neuronal activity. Table 1: Descriptions of ...chemogenetic receptors that confer more direct control of neurons through manipulation of ion channels...expression of AAV-delivered constructs can be controlled with cell-type specific promoters. Table 4 lists...unwanted cell types. Generating a FLEx switch to control expression of a chemogenetic reporter ensures that...N. C. (2024). Efficient opto- and chemogenetic control in a single molecule driven by FRET-modified bioluminescence...

...postivie or negative screens. The use of appropriate controls (positive and negative) is the responsibility ... Perform Next Generation Sequencing (NGS) on a control sample (no selection) In parallel, apply library...cells Compare NGS results between experimental and control cells Generate a list of gRNAs that disappear with...selection while the other serves as a non-selected control. These two populations are then sequenced using...Screening Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens New Tool for...

... and experimental resources that relate to the control of gene expression in the grasses. The TapRoot ...

...polymerase encoded in the E. coli genome (usually controlled by IPTG). For this IPTG-induced expression to...

...Ming G-L, King JR, Song H, Sweatt JD (2013) TET1 Controls CNS 5-Methylcytosine Hydroxylation, Active DNA...

...editing in their livers, while non-injected and control gRNA injected mice had no detectable editing. Additionally...

... in the hands of the Doench lab. Additionally, controls available in the system are validated and were...