We narrowed to 494 results for: control
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TypeCollection...CRISPR experiments and can be used as positive controls when working out a new protocol. Read more about...Weissman, J. S. (2014). Genome-Scale CRISPR-Mediated control of gene repression and activation. Cell , 159 (...
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Promoters
TypeGuide...vital component of expression vectors because they control the binding of the RNA polymerase to DNA. RNA polymerase... functional protein. Thus, the promoter region controls when and where in the organism your gene of interest... are proteins which recruit RNA polymerase and control and regulate the transcription of DNA into mRNA... bind to a promoter. Transcription is tightly controlled in both bacteria and eukaryotes by various DNA...consists of the sequence TTGACA, and this element controls the rate of transcription. Bacterial cells contain...Operons are a cluster of different genes that are controlled by a single promoter and operator. Operons are...operon in bacteria is an example of a negatively-controlled operon (Figure 3). A positive-repressible operon... -
Viral Vectors 101: Systemic Capsids
TypeBlog Post...neurons. (b) Genetic cargo can be customized to control transgene expression. Gene regulatory elements .../off genetic switch can be used for expression control. (c) Viral delivery methods include focused ultrasound...to be aware of before starting an experiment. Controlling expression As systemic capsids are… well, systemic...tissues and cell types. If desired, spatiotemporal control of transgene expression or activity can be achieved... -
The Breast Cancer Microenvironment: A Tumor’s Backstage Team
TypeBlog Post...decrease the expression of TAK 1, an enzyme that controls apoptosis and regulates transcription. Without... cannot undergo apoptosis, thus multiplying uncontrollably and building up tumors. “Friendly” microenvironment... -
Viral Vectors 101: Calcium Sensors
TypeBlog Post...fluorescent state only by simultaneous (1) user-controlled illumination with violet light and (2) exposure... -
Choosing the B(right)est Fluorescent Protein: Photostability
TypeBlog Post...substrate. These in vitro methods allow for a well-controlled environment, but they do not mimic the natural... -
Rosella: A Fluorescent pH-Biosensor for Studying Autophagy
TypeBlog Post...this is indicative of protein degradation). To control for protein degradation due to other pathways, ... -
CRISPRainbow and Genome Visualization
TypeBlog Post...of the Pitfalls When Using Fluorescent Proteins Control Protein Activity with GFP Resources on Asddgene.org... -
Plasmids 101: CcdB - The Toxic Key to Efficient Cloning
TypeBlog Post...PubMed PMID: 1324324. 6. Tam, JE. and Kline, BC. “Control of the ccd operon in plasmid F.” J Bacteriol. 1989... -
Popular Retroviral Vectors and Their Uses in Scientific Research
TypeBlog Post...inactive (dead), human codon-optimized Cas9 under the control of Murine Stem Cell retrovirus promoter for mammalian... -
Hot Plasmids and Viral Preps - March 2021
TypeBlog Post...single Cas9-VPR in Saccharomyces cerevisiae. To control Cas9 activity and influence DNA repair pathways... -
Plasmids 101: FLEx Vectors
TypeBlog Post...transduced reporter gene. Generating a FLEx switch to control expression of an optogenetic reporter would ensure... -
Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening
TypeBlog Post... Luke A., et al. "Genome-scale CRISPR-mediated control of gene repression and activation." Cell 159.3 ... -
Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing
TypeBlog Post... etc)? Did they perform all of the appropriate controls? Refer back to the methods section for finer points... -
Science Guides
TypeGuide...selectively control and investigate cell signaling pathways. Receptors are remotely controlled via small...small molecules, which allows for specific control of activity. Read More CRISPR Class 2 C lustered R egularly...research utilize light to detect, measure, and control molecular signals and cells in order to understand... -
Chemogenetics Guide
TypeGuide... However, neuronal control through PSAMs and LMOs is direct, while neuronal control of DREADDs is indirect...endogenous signaling, therefore allowing precise control over the cell population they are targeted to. ...used in the same organism to allow bidirectional control of neuronal activity. Table 1: Descriptions of ...chemogenetic receptors that confer more direct control of neurons through manipulation of ion channels...expression of AAV-delivered constructs can be controlled with cell-type specific promoters. Table 4 lists...unwanted cell types. Generating a FLEx switch to control expression of a chemogenetic reporter ensures that...N. C. (2024). Efficient opto- and chemogenetic control in a single molecule driven by FRET-modified bioluminescence... -
Making CRISPR Plasmids Using Fragmid
TypeBlog Post... in the hands of the Doench lab. Additionally, controls available in the system are validated and were... -
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation
TypeBlog Post...Ming G-L, King JR, Song H, Sweatt JD (2013) TET1 Controls CNS 5-Methylcytosine Hydroxylation, Active DNA... -
Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells
TypeBlog Post...editing in their livers, while non-injected and control gRNA injected mice had no detectable editing. Additionally... -
Quickest Way to Deposit Plasmids: The Deposit Spreadsheet
TypeBlog Post...MTA) approval Send plasmids to Addgene Quality control In this post, we’ll explain how to use our deposit...