We narrowed to 364 results for: gne
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TypeGuide...Increasing Cas9 PAM flexibility CRISPR systems designed for more precise edits require PAM sequences in...with base editing. While many base editors are designed to work in a very narrow window proximal to the...to target paralogs or lncRNAs . Guide RNAs are designed in silico and synthesized (Figure 8A), then cloned...
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Cloning
TypeGuide...stranded DNA fragments can be used, so if properly designed, any insert fragment (PCR product or synthesized... backbone independent of how it was originally designed to be cloned into. A drawback of this system is... -
Plan Your Experiment
TypeGuide... System Once you have selected your target and designed your gRNA(s), the next step is to choose how to... -
Adenovirus Guide
TypeGuide...be harvested about 7–10 days later. Vogelstein designed multiple shuttle plasmids for different purposes...