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We narrowed to 861 results for: lic

Showing: 361 - 380 of 861 results

  1. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...immunofluorescence? Generally speaking, IF is an application in which antibodies that have been joined to ...distribute antibodies validate them in a variety of applications, including IF (or IHC or ICC), and will share...will share their findings either through their publications or as a review on a company website. It is important...Resources on the Addgene blog Learn about research applications of antibodies in Introduction to Antibodies ... for Me? Read about another common antibody application in The Basics of Western Blotting Resources ...

  2. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...live cell imaging of second messengers cyclic di-GMP and cyclic AMP-GMP." Journal of the American Chemical...aptamer soup by exploring the development and applications of fluorescent RNA aptamers in Addgene’s collection...et al., 2017) and levels of the second messenger cyclic di-AMP in Gram-positive bacteria (Kellenberger ...fluorescent rna aptamer spinach2 for imaging of replication and immune-mediated changes in intracellular ...

  3. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...multimerization of the PCR primers can cause duplication of the primer sequence in the resulting plasmid...proximal to the target site, can identify these duplications (slightly larger bands sizes relative to the...percentage agarose gel (~3%). Note that primer duplication will evade detection in the initial RFLP analysis...because the introduced restriction site is also duplicated. Validating your mutagenized plasmids Once constructs...identified (and confirmed to be free of primer duplications), final validation is performed by sequencing...

  4. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...approach may also be applicable to a number of rare genetic diseases caused by splicing defects, including...estimated that exon skipping therapies would be applicable to 80% of DMD patients. Figure 1: Exon...from Osterout et al. (2015b) under a CC-BY 4.0 license. Long et al., Nelson et al., and Tabebordbar...Exon-skipping antisense oligonucleotides to correct missplicing in neurogenetic diseases. Nucleic Acid Ther. ...

  5. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    ... for PhDs or postdocs. Having this information publicly available may realign trainee expectations with...Publishing Panel brought in editors from a variety of publications as well as young PIs and all gave advice on ...blogging, write reviews, or write for a university publication, improving your general writing skills will help...cognizant of this during my PhD. Submitting a publication doesn’t have to be like throwing it into a black...Science The Future of Research Website Additional Publications of Interest Can Be Found Here Resources at ...

  6. Synthetic Biology - Overview

    Type
    Collection
    ...vectors. Metabolism Browse plasmids related to metabolic pathways and auxiliary components. Networks and...

  7. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...adapted from Andrews et al., 2019 under CC-BY 4.0 license.   Selecting your antibodies When selecting...primary antibodies that have been validated in IF applications (find IHC validated or ICC validated antibodies...978-1-4939-8935-5_26 Jambor, H., Antonietti, A., Alicea, B., Audisio, T. L., Auer, S., Bhardwaj, V., Burgess...informative image-based figures for scientific publications. PLOS Biology, 19(3), e3001161. https://doi....Immunocytochemistry Protocols for other antibody applications...

  9. Activate Addgene's "Find Plasmids" Badge on Pubmed Abstracts

    Type
    Blog Post
    ...within that article you’ll find new plasmids. Simply click on the badge to be taken directly to the plasmids...account on PubMed. Once you have an account, simply click on your account name in the top right corner of ...PubMed to navigate to your account settings. Next, click on the link in the “NCBI Site Preferences” box. ...Preferences page, navigate to “PubMed Preferences” and click on the “Filters & Icons” link (see image to the ...

  11. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ... wanted to inactivate the gene BRAF (a kinase implicated in several human cancers) in A549 cells (a human... Cas9 expressing lentivirus at the indicated multiplicity of infection (MOI). Anti-vinculin Western blotting...need to use FACS (like they did in the original publication). I thought I could see GFP expression with a...expression, but I didn’t. 2. In the original publication they see GFP expression in about 20% of their...an MOI of 0.3, as recommended by the original publication. Pro-Tip: 1. Use multiple gRNAs to target different...

  12. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    ...immunoprecipitation applications, but none of the traditional tags were suitable for both applications. To prove...These tags are easy to use in multiple common applications. Due to their limited effects on native protein...particularly in single-molecule or high-throughput applications. Perhaps IT5 and IT6 will even replace our older...

  13. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    ...Since the first research applications of GFP were published in the 1990s, biologists have spent a lot... Sattarzadeh et al., Sci Rep. 2015 CC BY 4.0 Applications and limitations of GFP photoconversion One concern...2):131-3.  PubMed. Find plasmids from this publication at Addgene. Subach OM, Patterson GH, Ting LM...nmeth.1664. PubMed. Find plasmids from this publication at Addgene.   Addgene’s Fluorescent Protein...

  14. Scientific Sharing in the Time of COVID-19: Databases and Resources

    Type
    Blog Post
    ...funding and volunteer opportunities, protocols, publications, and more to accelerate the pace of research...is based in Canada, much of the information is applicable to researchers worldwide. “COVID-19 has spread...advance COVID-19 research to hopefully help guide policies and ultimately treatments in a timely fashion.... a significant impact on both clinical care and public health for this current COVID-19 pandemic, and ...

  15. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    ... QC/QA team as a DNA Sequencing Intern. The application process When I first saw the comprehensive job...immediately clear that Addgene wanted to ensure applicants understood what the expectations were for the...the intended position. After submitting my application, I was contacted to participate in a brief fifteen...the depositing labs intended. With the use of applications like SnapGene and Geneious, I was able to determine...

  16. SunTag and Fluorescent Imaging

    Type
    Blog Post
    ...modified the GCN4 sequence to increase its alpha-helical structure and stability, creating v4 SunTag. Since...aggregation, it’s recommended for most imaging applications. What can you do with SunTag? Tanenbaum et al...flames,” it’s clear that the system is broadly applicable across many areas of research. Beyond fluorescence...activation of target genes, but we’ll save that application for another day! References 1. Tanenbaum, Marvin...

  17. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    ...simply writing down your data doesn’t guarantee a publication. There is an art to composing manuscripts and...field of microbiology with multiple high-profile publications. My classmates consisted of graduate students...well as being critiqued added an extra level of application not present in the online class.   Four ways ...paper will be generated. After you complete a publication quality figure, produce the figure, its legend...

  18. Sensing Neuronal Dopamine

    Type
    Blog Post
    ...experiments The Tian lab used dLight1 both in brain slices and freely behaving mice to gather the following...and concentration of released DA in mouse brain slices Visualization of DA release dynamics during locomotion...GRABDA in drosophila, zebrafish, and mouse brain slices and freely moving mice to gather the following ...from a single dopaminergic fiber in mouse brain slices Demonstrate DA detection in a single neuron in ...

  19. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ...containing only the sequences required for viral replication and transcription as well as reporter genes. ... membranes. Read the full post for potential applications of R-bodies and to find a video of them in action...these libraries from the post and the associated publication. Optimizing Donor DNA for Enhanced CRISPR Genome... to your DNA sequence of interest. Potential applications of the system include activation and repression...

  20. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    ... (AmpR) and origins of replication (ori and f1 ori) which allow it to replicate.   How can I prevent...the bacterial selection marker and origin of replication. During bacterial culture, only the plasmid with...the antibiotic resistance gene and origin will replicate, and the transgene will be lost. Over time, bacteria...
Showing: 361 - 380 of 861 results